FB2024_03 , released June 25, 2024
Insertion: Dmel\PBac{RB}αTub84De02691
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General Information
Symbol
Dmel\PBac{RB}αTub84De02691
Species
D. melanogaster
Name
FlyBase ID
FBti0101432
Description
Inserted Element
LINE ID

e02691

Affected gene(s)
Viability / fertility
Associated allele(s)
Stock availability
Genomic Location
Chromosomal Location
3R ( 84D9 )
Sequence Location
3R:7,530,674..7,530,674
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Description

PBac{RB} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.

A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{RB}. The PBac{RB} construct carries the w+mC mini-white marker, a single long (199-bp) Scer\FRT site, and a "splice trap," a splice acceptor site derived from a D. melanogaster gene. The FRT site allows Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions.

Detailed Mapping Data
Chromosome (arm)
Sequence Location
3R:7,530,674..7,530,674
Orientation
Cytological location (computed by FlyBase)
84D9 (inferred by FlyBase from sequence location)
Cytological location (reported)
Insertion into Natural transposon
Comments concerning location

The Gene Disruption project determined the insertion site of PBac{RB}e02691 to be Release 3 genomic coordinate 3356415 on arm 3R.

Exelixis, Inc. determined the insertion site of PBac{RB}e02691 to be Release 3 genomic coordinate 3356248 on chromosome arm 3R.

Sequence Data
Flanking sequence
Inserted Element
Size (Kb)

5.971

Component Allele(s)
Molecular map
Affected Gene(s)
Insertion may affect gene
Variant Molecular Consequences
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
PBac{RB}αTub84De02691
Name Synonyms
Secondary FlyBase IDs
    References (5)