FlyBase Experimental Tool Report: TagRFP-T

FB2024_02 , released April 23, 2024

FB2024_02 , released April 23, 2024

Experimental tool: TagRFP-T

General Information

Symbol

TagRFP-T

FlyBase ID

FBto0000099

Name

TagRFP-T fluorescent protein

Description

Description

TagRFP-T is a monomeric red fluorescent protein with an excitation peak of 555nm and an emission peak of 584nm in vitro. It is an artificial derivative of the naturally occurring fluorescent protein encoded by the Entacmaea quadricolor eqFP578 gene, derived by mutagenesis of TagRFP, containing the mutation S158T relative to TagRFP. It is approximately nine-fold more photostable than TagRFP (PMID:18454154).

Uses

red fluorescent protein

External Crossreferences and Linkouts

FPbase - A database for users of fluorescent proteins.

LF3LJ

Related experimental tools

Transgenic Constructs

Encodes tool (7)

Transgenic construct(s)

Component allele

Reg. region

Encoded product / tool

Tagged with

Also carries

Stocks

M{UASp-Lifeact.TagRFP-T}

Equa\eqFP578^{TagRFP-T.UASp.cHa.Lifeact}

UASp

TagRFP-T: red fluorescent protein

Lifeact: specific binding tool

P{UAS-GalT-TagRFP-T}

Equa\eqFP578^{TagRFP-T.UAS.Tag:Golgi(hB4GALT1)}

UAS

TagRFP-T: red fluorescent protein

Tag:Golgi(hB4GALT1): Golgi protein localization tag

P{UAS-ManII-TagRFP-T}

Equa\eqFP578^{TagRFP-T.UAS.Tag:Golgi(ManII)}

UAS

TagRFP-T: red fluorescent protein

Tag:Golgi(ManII): Golgi protein localization tag

P{Hsp83-NLS-2xMCP-2xTagRFP-T}

Equa\eqFP578^{TagRFP-T.Hsp83.Tag:MCP,Tag:NLS(Unk)}

Hsp83

TagRFP-T: red fluorescent protein

Tag:NLS(Unk): nuclear protein localization tag
Tag:MCP: specific binding tool

P{nanos-MCP.TagRFP-T.NLS}

Equa\eqFP578^{TagRFP-T.nanos.Tag:MCP,Tag:NLS(Unk)}

nanos

TagRFP-T: red fluorescent protein

Tag:NLS(Unk): nuclear protein localization tag
Tag:MCP: specific binding tool

P{nanos-MCP.TagRFP-T}

Equa\eqFP578^{TagRFP-T.nanos.Tag:MCP}

nanos

TagRFP-T: red fluorescent protein

Tag:MCP: specific binding tool

P{UASp-TagRFP-T.2xFYVE}

Equa\eqFP578^{TagRFP-T.UASp.Tag:2xFYVE}

UASp

TagRFP-T: red fluorescent protein

Tag:2xFYVE: small molecule sensor
protein localization tag

Tagged with the tool (16)

Transgenic construct(s)

Component allele

Reg. region

Encoded product / tool

Tagged with

Also carries

Stocks

P{UAS-OptoSOS}

Hsap\SOS2^{cat.UAS.TagRFP-T,Tag:SSPB}

UAS

Hsap\SOS2

TagRFP-T: red fluorescent protein
Tag:SSPB: specific binding tool

P{UAS-GALNT2.TagRFP-T}

Hsap\GALNT2^UAS.TagRFP-T

UAS

Hsap\GALNT2

TagRFP-T: red fluorescent protein

M{Ubi-Dlg5.TagRFP-T}

Dlg5^{Ubi-p63E.TagRFP-T}

Ubi-p63E

Dlg5

TagRFP-T: red fluorescent protein

P{Dlg5-TagRFP-T}

Dlg5

Dlg5

TagRFP-T: red fluorescent protein

P{matTub-grp.TagRFP-T}

grp^{matTub.TagRFP-T}

grp

TagRFP-T: red fluorescent protein

P{nanos-scFv(GCN4).TagRFP-T.NLS}

Zzzz\scFv^{GCN4.nanos.TagRFP-T.Tag:NLS(Unk)}

nanos

scFv(GCN4): single-chain variable fragment antibody

TagRFP-T: red fluorescent protein
Tag:NLS(Unk): nuclear protein localization tag

P{PCNA.TagRFP-T}

PCNA

TagRFP-T: red fluorescent protein

P{TagRFP-T-Fib}

Fib

Fib

TagRFP-T: red fluorescent protein

P{UASC-Myc-TagRFP-T-caz}

caz^{UAS.BID.Tag:MYC,TagRFP-T}

UAS

caz

Tag:MYC: epitope tag
TagRFP-T: red fluorescent protein

P{UAS-Eb1.TagRFP-T}

Eb1^UAS.TagRFP-T

UAS

Eb1

TagRFP-T: red fluorescent protein

P{UAS-γTub23C.TagRFP-T}

γTub23C^UAS.TagRFP-T

UASt

γTub23C

TagRFP-T: red fluorescent protein

P{UAS-Grasp65.TagRFP-T}

Grasp65^UAS.TagRFP-T

UAS

Grasp65

TagRFP-T: red fluorescent protein

P{UASp-Vinc.FL.TagRFP-T}

Vinc^{FL.UAS.TagRFP-T}

UASp

Vinc

TagRFP-T: red fluorescent protein

P{UAS-RFeSP.Noble.TagRFP-T}

RFeSP^UAS.TagRFP-T

UAS

RFeSP

TagRFP-T: red fluorescent protein

P{UAS-Smu1.myc.TagRFP-T}

Smu1^{UAS.Tag:MYC,TagRFP-T}

UAS

Smu1

Tag:MYC: epitope tag
TagRFP-T: red fluorescent protein

P{UAS-upd2.TagRFP-T}

upd2^UAS.TagRFP-T

UAS

upd2

TagRFP-T: red fluorescent protein

Insertions ( 14 )

Insertion

Encoded product / tool

Tagged with

Also carries

Stocks

Associated Alleles

Mi{PT-TRH.0}MI08578a

Tag:HA: epitope tag
TagRFP-T: red fluorescent protein

para^{MI08578-TRH.0}

Mi{PT-TRH.0}MI08578b

Tag:HA: epitope tag
TagRFP-T: red fluorescent protein

para^{MI08578-TRH.0}

Mi{PT-TRH.1}Rfx^{MI00053-TRH.1}

Tag:HA: epitope tag
TagRFP-T: red fluorescent protein

Rfx^{MI00053-TRH.1}

TI{T-STEP.TagRFP-T}Csas^KI

TagRFP-T: red fluorescent protein

RSRT: recombinase target site

Csas^KI

TI{T-STEP.TagRFP-T}Ocrl^KI

TagRFP-T: red fluorescent protein

RSRT: recombinase target site

Ocrl^KI

TI{T-STEP.TagRFP-T}Vps35^D628N

TagRFP-T: red fluorescent protein

RSRT: recombinase target site

Vps35^D628N

TI{T-STEP.TagRFP-T}Vps35^KI

TagRFP-T: red fluorescent protein

RSRT: recombinase target site

Vps35^KI

Mi{PT-TRH.0}CadN^{MI00393-TRH.0}

Tag:HA: epitope tag
TagRFP-T: red fluorescent protein

CadN^{MI00393-TRH.0}

Mi{PT-TRH.0}rhea^{MI00296-TRH.0}

Tag:HA: epitope tag
TagRFP-T: red fluorescent protein

rhea^{MI00296-TRH.0}

Mi{PT-TRH.1}comm^{MI00380-TRH.1}

Tag:HA: epitope tag
TagRFP-T: red fluorescent protein

comm^{MI00380-TRH.1}

Mi{PT-TRH.2}nord^{MI06414-TRH.2}

Tag:HA: epitope tag
TagRFP-T: red fluorescent protein

nord^{MI06414-TRH.2}

TI{T-STEP.TagRFP-T}Dap160^TSTEP

TagRFP-T: red fluorescent protein

RSRT: recombinase target site

Dap160^TSTEP

TI{T-STEP.TagRFP-T}Syt4^KI

TagRFP-T: red fluorescent protein

RSRT: recombinase target site

Syt4^KI

TI{T-STEP.TagRFP-T}Syt4^KI-EGFP

TagRFP-T: red fluorescent protein

RSRT: recombinase target site

Syt4^KI-EGFP

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

T:Equa\eqFP578^TagRFP-T

(FlyBase, 1992-)

T:Zzzz\eqFP578^TagRFP-T

(FlyBase, 1992-)

TagRFP-T

Name Synonyms

TagRFP-T fluorescent protein

Secondary FlyBase IDs

FBal0321176
FBal0320562
FBal0261128

References (32)

Report Sections