Tag:LEXY consists of the LOV2 domain of the Avena sativa Phototropin 1 (PT1) gene, including the C-terminal Jα-helix and LOV core domain, in which the C-terminal Jα-helix has been converted into an artificial nuclear export sequence (NES). In the dark, the NES is tightly packed against the LOV core and is thus inactive. Upon illumination with blue light, the Jα-helix unfolds, exposing the NES, and allowing export from the nucleus of a protein of interest to which Tag:LEXY is fused (adapted from PMID:26853913).
