FB2024_03 , released June 25, 2024
Recombinant Construct: P{GAL4-twi.B}
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General Information
Symbol
P{GAL4-twi.B}
FlyBase ID
FBtp0000758
Feature type
transgenic_transposable_element
Component Allele(s)
Scer\GAL4twi.PB
Also Known As
twi-GAL4, twist-GAL4, twiGAL4, twistGal4, P{w+mC=tw-GAL4}
Size
Expression Data
GAL4 reporter/driver
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
w+mW.hs
Component allele Scer\GAL4twi.PB
Transgenic product class
Component
Use(s)
Regulatory region(s)
twi
Encoded product / tool
GAL4
Description

GAL4 driver expression is driven by a twi enhancer and promoter included within a 1.4kb HindIII-ClaI fragment that includes the elements necessary for proper twi expression.

(Baylies and Bate, 1996)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
mesoderm
(Staehling-Hampton et al., 1994)
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
mesoderm
(Jenkins et al., 2003)
embryonic Malpighian tubule | restricted
(Denholm et al., 2003)
embryonic stage 8
mesoderm
(Trébuchet et al., 2019)
embryonic stage 12
embryonic precursor cell of adult muscle
(Massarwa et al., 2007)
Additional Information
Statement
Reference

Drives mesodermal expression.

(FlyBase Curators, 2017)

The expression of twi and byn in the Malpighian tubules was assayed using the GAL4/UAS system. Reporter gene expression was detected in subsets both the principle MpT cells and the stellate cell population of embryonic tubules suggesting a mesodermal origin of some of these cells.

(Denholm et al., 2003)
 
Marker for
Reflects expression of
twi
(Trébuchet et al., 2019, Massarwa et al., 2007, Denholm et al., 2003, Staehling-Hampton et al., 1994)
Reporter construct used in assay
Progenitors and Descendants
Comments

report comment: Selectable marker not specified.

(Staehling-Hampton et al., 1994)

report comment: Included as non-attributed correction.

(Baylies and Bate, 1996)

construct_comment: Included as non-attributed correction.

(Baylies and Bate, 1996)
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
80574
w*; P{GAL4-twi.B}2
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (13)
Reported As
Symbol Synonym
P{GAL4-twi.B}
(FlyBase, 1996-)
P{w+mC=tw-GAL4}
(Casso et al., 2000, Casso et al., 1999)
Twi-GAL4
(Ruiz-Gomez et al., 2002, Ruiz-Gomez et al., 2000)
twi-GAL4
(Ruan et al., 2024, Bothe et al., 2014, Nowak et al., 2012, Patel et al., 2012, Wong et al., 2008, Xu et al., 2008, Massarwa et al., 2007, Beckett and Baylies, 2006, Kolesnikov and Beckendorf, 2005, Miskolczi-McCallum et al., 2005, Denholm et al., 2003, Carmena et al., 2002, Lo et al., 2002, Artero et al., 2001, Cai et al., 2001, Fossett et al., 2000, Garcia-Alonso et al., 2000, Brennan et al., 1999)
twi-Gal4
(Samata et al., 2020, Ciglar et al., 2014, Dobi et al., 2014, Metzger et al., 2012, Liotta et al., 2007, Muratoglu et al., 2007, Tokusumi et al., 2007, Duan and Nguyen, 2006, Wawersik et al., 2005, Gajewski et al., 2001, Kammerer and Giangrande, 2001, Halfon et al., 2000, Gajewski et al., 1999, Schulz and Gajewski, 1999)
twi::Gal4
(Schumacher et al., 2004)
twiGAL4
(Cox and Baylies, 2005)
twiGal4
(Trébuchet et al., 2019, Muratoglu et al., 2006)
twist-GAL4
(Carrasco-Rando et al., 2011, Massarwa et al., 2007, Mathews et al., 2006, Cox et al., 2005, Hoehne et al., 2005, Kuzin et al., 2005, Kuzin et al., 2005, DeFalco et al., 2004, Tapanes-Castillo and Baylies, 2004, Castanon et al., 2001, Gunthorpe et al., 1999, Staehling-Hampton et al., 1994)
twist-Gal4
(Schulman et al., 2014, Boukhatmi et al., 2012, Enriquez et al., 2012, Mathews et al., 2005, Artero et al., 2003, Jenkins et al., 2003, Kremser et al., 1999)
twist-gal4
(Zimmermann et al., 2006)
twistGAL4
(San Martin and Bate, 2001)
twistGal4
(Fossett et al., 2001)
Secondary FlyBase IDs
    References (79)