FB2024_03 , released June 25, 2024
Recombinant Construct: P{lacZ.gypsy-su(Hw)BR.(FRT.PTS).iab8}
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General Information
Symbol
P{lacZ.gypsy-su(Hw)BR.(FRT.PTS).iab8}
FlyBase ID
FBtp0011887
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Description

Transgenic construct used to study mechanisms involved in long-range enhancer-promoter interactions. Contains a basal P\T promoter upstream of the lacZ reporter. A 360bp BamHI-BglII fragment containing gypsy insulator sequence, an FRT cassette that contains a 625bp minimal Abd-B promoter targeting sequence (PTS), and a 1.6kb IAB8 enhancer fragment have been inserted over 5kb downstream of the lacZ transcription unit.

Construct components
Component
Use(s)
Encoded product / tool
Component allele Abd-B-PTS625
Transgenic product class
Component
Use(s)
Encoded product / tool
Description

Construct: A 625bp minimal Abd-B-PTS fragment.

Component allele gypsy\su(Hw)BR360
Transgenic product class
Component
Use(s)
Encoded product / tool
Description

Construct: A 360bp BamHI-BglII fragment containing the gypsy\su(Hw)BR.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{lacZ.gypsy-su(Hw)BR.(FRT.PTS).iab8}
P{lacZ.gypsy\su(Hw)BR.Scer\FRT.PTS.iab8}
Secondary FlyBase IDs
    References (2)