FB2024_03 , released June 25, 2024
Recombinant Construct: P{W32(FRT.PTS.Fab-8)iab-8}
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General Information
Symbol
P{W32(FRT.PTS.Fab-8)iab-8}
FlyBase ID
FBtp0016597
Feature type
transgenic_transposable_element
Component Allele(s)
Abd-B-PTS290bp
Fab-8tmr
iab-8PREtmr
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Description

Transgenic construct used to study long-range enhancer-promoter interactions in vivo. Contains two divergently transcribed reporters; w+mC and lacZ with a minimal P\T promoter. A 5.3 tmr fragment containing the iab-8 enhancer, Fab-8 insulator and 290bp of the 625bp promoter targeting sequence (PTS) has been inserted downstream of the lacZ reporter, oriented such that the PTS sequence is proximal to lacZ. Two FRT sites have been inserted flanking the PTS and Fab-8 sequences (allowing them to be excised using FLP recombinase).

(Lin et al., 2003)
Construct components
Component
Use(s)
Encoded product / tool
lacZ
Also carries
FRT
Component allele Abd-B-PTS290bp
Transgenic product class
Component
Use(s)
Encoded product / tool
Abd-B-PTS
Description
Component allele Fab-8tmr
Transgenic product class
Component
Use(s)
Encoded product / tool
Fab-8
Description

Construct: Contains the full length 9.5kb tmr (transvection mediating region) from the Abd-B region, which includes Fab-8 sequences.

(Zhou and Levine, 1999)
Component allele iab-8PREtmr
Transgenic product class
Component
Use(s)
Encoded product / tool
iab-8PRE
Description

Construct: Contains the full length 9.5kb tmr (transvection mediating region) from the Abd-B region, which includes iab-8PRE sequences.

(Zhou and Levine, 1999)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
P{W32(FRT.PTS.Fab-8)iab-8}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (3)