FB2024_03 , released June 25, 2024
Recombinant Construct: P{UAS-SCAT3}
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General Information
Symbol
P{UAS-SCAT3}
FlyBase ID
FBtp0021422
Feature type
transgenic_transposable_element
Component Allele(s)
Avic\GFPSCAT3.UAS
Also Known As
UAS-SCAT3
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Construct components
Component allele Avic\GFPSCAT3.UAS
Transgenic product class
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Avic\GFP
Description

UASt sequences drive expression of "SCAT3", a genetically encoded reporter for caspase activation. SCAT3 comprises the ECFP and Venus proteins linked by an 18 amino acid peptide that includes DEVD, the caspase-3 cleavage sequence. Caspase activation can be monitored by changes in the fluorescence energy transfer (FRET) from ECFP to Venus; cleavage of the linker peptide by activated caspase abolishes the FRET (details from PMID:12527749).

(Kanuka et al., 2005)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
P{UAS-SCAT3}
SCAT3
(Kanuka et al., 2005)
UAS-SCAT3
(Fujisawa et al., 2020, Kuranaga et al., 2011, Nakajima et al., 2011, Ribeiro et al., 2007)
UAS-SCAT3DEVD
(Shinoda et al., 2019)
pUAST-SCAT3
(Kuranaga et al., 2006, Kanuka et al., 2005)
Secondary FlyBase IDs
    References (8)