FlyBase Recombinant Construct Report: P{tub-Rad21.550TEV-myc}

A tubulin promoter drives expression of vtd, which is tagged 12bp upstream of the translational stop codon with 10 copies of Tag:MYC. In addition, an SpeI site has been introduced into the vtd coding region after amino acid residue 550, and 3 tandem copies of the Tag:CS(TEVp) protein cleavage tag have been introduced into this site (this sequence allows cleavage of the vtd protein by TEV\NIa). The portion of the construct containing the vtd coding region and a 3'UTR sequence is flanked by an FRT cassette.

(Pauli et al., 2008)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

vtd^{550TEV.tub.Tag:CS(TEVp),Tag:MYC}

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (1)

Stocks containing insertion(s) of this recombinant construct

Bloomington

27614

w^*; vtd^ex15 P{tub-Rad21.550TEV-myc}3

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

P^w<up>+, tubpr < Rad21(550-3TEV)-myc₁₀ < SV40</up>

(Pauli et al., 2010)

P{tub-Rad21.550TEV-myc}

(FlyBase, 1992-)

P{w⁺, tubpr<Rad21(550-3TEV)-myc₁₀<SV40}

(Pauli et al., 2008)

Rad21(550-3TEV)-myc

(Oliveira et al., 2010)

Rad21-(TEV-550)

(Urban et al., 2014)

Rad21TEV

(Urban et al., 2014)

tub-Rad21(550-3TEV)-myc

(Pauli et al., 2008)

tubpr-Rad21(550-3TEV) -myc10

(Piskadlo et al., 2017)

tubpr-Rad21(550-3TEV)-myc10

(Carvalhal et al., 2018)

Secondary FlyBase IDs

References (8)

Report Sections

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