FlyBase Allele Report: Dmel\vtd[550TEV.tub.Tag:CS(TEVp),Tag:MYC]

General Information

Symbol

Dmel\vtd^{550TEV.tub.Tag:CS(TEVp),Tag:MYC}

Species

D. melanogaster

Name

FlyBase ID

FBal0230183

Feature type

allele

Associated gene

Dmel\vtd

Associated Insertion(s)

Carried in Construct

P{tub-Rad21.550TEV-myc}

Key Links

Transgenic product class

wild_type

(Pauli et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Pauli et al., 2008)

Mutagen

in vitro construct

(Pauli et al., 2008)

Progenitor genotype

Carried in construct

P{tub-Rad21.550TEV-myc}

Cytology

Description

A tubulin promoter drives expression of vtd, which is tagged 12bp upstream of the translational stop codon with 10 copies of Tag:MYC. In addition, an SpeI site has been introduced into the vtd coding region after amino acid residue 550, and 3 tandem copies of the Tag:CS(TEVp) protein cleavage tag have been introduced into this site (this sequence allows cleavage of the vtd protein by TEV\NIa). The portion of the construct containing the vtd coding region and a 3'UTR sequence is flanked by an FRT cassette.

(Pauli et al., 2008)

Allele components

Component

Use(s)

Encoded product / tool

Tagged with

Also carries

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Females in which vtd^{550TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC} is the sole source of vtd protein (carrying vtd^{550TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC} in a vtd^unspecified background) show premature synaptonemal complex disassembly in egg chambers after cleavage of the vtd^{550TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC} protein by expression of Zzzz\NIa^{S219V.Scer\UAS.P\T.T:Uuuu\nls6,T:SV5\V5} under the control of Scer\GAL4^nos.PG.

(Urban et al., 2014)

Larvae in which vtd^{550TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC} is the sole source of vtd protein (carrying vtd^{550TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC} in a vtd^ex3/vtd^ex15 background) show defects in the salivary glands after cleavage of the vtd^{550TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC} protein by expression of Zzzz\NIa^{Scer\UAS.T:SV5\V5,T:SV40\nls2} under the control of Scer\GAL4^hs.2sev at 18[o]C; the salivary glands are smaller than normal and have smaller nuclei than normal. The number of cells per salivary gland is unaltered.

Animals in which vtd^{550TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC} is the sole source of vtd protein (carrying vtd^{550TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC} in a vtd^ex3/vtd^ex15 background) show defects in pruning of γ neurons in the mushroom body after cleavage of the vtd^{550TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC} protein by expression of Zzzz\NIa^{Scer\UAS.T:SV5\V5,T:SV40\nls2} under the control of Scer\GAL4^H24; larval γ neurons initially project into the dorsal and medial lobes as occurs in wild type, but at 24 hours after puparium formation, axon projections persist in the mutant medial and dorsal lobes and unpruned dendrites are seen (control animals have pruned the medial and dorsal axon projections of the γ neurons as well as their dendrites at this time). At 4 days after pupariation, the persisting γ neuron projections are often disorganised and mistargeted in the mutant pupae.

(Pauli et al., 2008)

Animals in which vtd^{550TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC} is the sole source of vtd protein (carrying vtd^{550TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC} in a vtd^ex15 background) show developmental arrest and lethality after cleavage of the vtd^{550TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC} protein by expression of Zzzz\NIa^{Scer\UAS.T:SV5\V5,T:SV40\nls2} under the control of either Scer\GAL4^elav.PLu or Scer\GAL4^nSyb.PS.

Animals in which vtd^{550TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC} is the sole source of vtd protein (carrying vtd^{550TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC} in a vtd^ex15 background) show locomotion defects after cleavage of the vtd^{550TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC} protein by expression of Zzzz\NIa^{Scer\UAS.T:SV5\V5,T:SV40\nls2} under the control of Scer\GAL4^Cha.7.4; third instar mutant larvae have severely reduced locomotion compared to controls. The mutant larvae curve repetitively, frequently turn their heads and even move backwards.

(Pauli et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

vtd^{550TEV.tub.Tag:CS(TEVp),Tag:MYC} rescues vtd^ex15

(Pauli et al., 2008)

Comments

vtd^{550TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC} rescues the lethality of vtd^ex15 animals.

(Pauli et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

27614

w^*; vtd^ex15 P{tub-Rad21.550TEV-myc}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

vtd^{550TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC}

vtd^{550TEV.tub.Tag:CS(TEVp),Tag:MYC}

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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