UASt regulatory sequences drive expression of TEVp, tagged at the N-terminal end with Tag:NLS(SV40-largeT) and Tag:V5 (one copy each) and at the C-terminal end with Tag:NLS(SV40-largeT) (two copies).
Expression of Zzzz\NIaScer\UAS.T:SV5\V5,T:SV40\nls2 under the control of Scer\GAL4F4 (by heat-shock to inactivate Scer\GAL80ts.αTub84B) in a vtdex15 mutant background results in a drastic reduction of salivary gland chromosome puffing at band 74. Puffs at band 75 were little affected 2 hours after expression was induced, although puffing was reduced by 4 hours after expression.