FB2024_03 , released June 25, 2024
Allele: Dmel\vtd271TEV.tub.Tag:CS(TEVp),Tag:MYC
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General Information
Symbol
Dmel\vtd271TEV.tub.Tag:CS(TEVp),Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0230182
Feature type
allele
Associated gene
Dmel\vtd
Associated Insertion(s)
Carried in Construct
P{tub-Rad21.271TEV-myc}
Also Known As
rad21TEV
Key Links
Transgenic product class
wild_type
(Pauli et al., 2008)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Pauli et al., 2008)
Mutagen
in vitro construct
(Pauli et al., 2008)
Progenitor genotype
Carried in construct
P{tub-Rad21.271TEV-myc}
Cytology
Description

A tubulin promoter drives expression of vtd, which is tagged 12bp upstream of the translational stop codon with 10 copies of Tag:MYC. In addition, an SpeI site has been introduced into the vtd coding region after amino acid residue 271, and 3 tandem copies of the Tag:CS(TEVp) protein cleavage tag have been introduced into this site (this sequence allows cleavage of the vtd protein by TEV\NIa). The portion of the construct containing the vtd coding region and a 3'UTR sequence is flanked by an FRT cassette.

(Pauli et al., 2008)
Allele components
Component
Use(s)
Encoded product / tool
vtd
Tagged with
Tag:CS(TEVp)
Tag:MYC
Also carries
FRT
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Females in which vtd271TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC is the sole source of vtd protein (carrying vtd271TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC in a vtdunspecified background) show premature synaptonemal complex disassembly in egg chambers after cleavage of the vtd271TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC protein by expression of Zzzz\NIaS219V.Scer\UAS.P\T.T:Uuuu\nls6,T:SV5\V5 under the control of Scer\GAL4nos.PG.

      (Urban et al., 2014)

      Embryos expressing vtd271TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYCdisplay centromere disjoining, resulting in sister chromatids moving towards opposite poles.

      (Oliveira et al., 2010)

      Embryos in which vtd271TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC is the sole source of vtd protein (carrying vtd271TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC in a vtdex3 background) show defects in embryonic mitotic cycles after cleavage of the vtd271TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC protein by expression of Zzzz\NIaScer\UAS.T:SV5\V5,T:SV40\nls2 under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16; cells initially undergo mitotic arrest during cycle 14, and chromosomes show unstable kinetochore attachment. After the initial mitotic arrest, chromosome decondensation starts abruptly and unattached chromatids in the central region are cut by the cleavage furrow.

      (Pauli et al., 2008)

      Embryos in which vtd271TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC is the sole source of vtd protein (carrying vtd271TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC in a vtdex3 background) show defects in embryonic mitotic cycles after cleavage of the vtd271TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC protein by expression of Zzzz\NIaScer\UAS.T:SV5\V5,T:SV40\nls2 under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16; no normal metaphase, anaphase or telophase figures are seen at mitosis 14. Bipolar spindles form, but during prometaphase sister chromatids disjoin prematurely and often segregate to opposite poles. Condensed chromosomes fail to align on a metaphase plate and are found scattered throughout the cells.

      (Pauli et al., 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The presence of a TEV protease cleavable form of vtd (by expressing vtd271TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC and Zzzz\NIaScer\UAS.T:SV5\V5,T:SV40\nls2 in a vtdex3 mutant background) suppresses the chromosome segregation defects seen in embryos expressing aldScer\UAS.cAa under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 in a mad2G6595 mutant background. This suppression does not occur in the absence of Zzzz\NIaScer\UAS.T:SV5\V5,T:SV40\nls2.

      (Althoff et al., 2012)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      rad21TEV
      (Oliveira et al., 2010)
      vtd271TEV.tub.T:Zzzz\TEV.CS,T:Hsap\MYC
      vtd271TEV.tub.Tag:CS(TEVp),Tag:MYC
      Name Synonyms
      Secondary FlyBase IDs
        References (5)