UAS regulatory sequences drive expression of an inverted repeat that targets pLUC; there are two 5xUAS cassettes (one can be removed using P1cre recombinase).
P-element construct created in vivo: the hairpin-containing segment, carried in a pVALIUM1-derived plasmid, was introduced into flies and inserted via site-specific recombination into a genomic copy of P{CaryP}.
P-element construct created in vivo: the hairpin-containing segment, carried in a pVALIUM1-derived plasmid, was introduced into flies and inserted via site-specific recombination into a genomic copy of P{CaryP}.