FB2024_03 , released June 25, 2024
Recombinant Construct: P{10XUAS-IVS-Syn21-GFP-p10}
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General Information
Symbol
P{10XUAS-IVS-Syn21-GFP-p10}
FlyBase ID
FBtp0073107
Feature type
transgenic_transposable_element
Component Allele(s)
Avic\GFPIVS.Syn21.p10.10xUAS
Also Known As
10XUAS-IVS-Syn21-GFP-p10, pJFRC81, JFRC81-10XUAS-IVS-Syn21-GFP-p10, pJFRC81-10xUAS-IVS-Syn21-GFP-p10
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Avic\GFPIVS.Syn21.p10.10xUAS
Transgenic product class
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
EGFP
Description

Ten copies of the UAS site followed by an Hsp70 basal promoter are fused upstream of EGFP which has been optimised for Drosophila codon usage. A small intron derived from the Mhc gene followed by a synthetic AT-rich 21bp sequence made by combining the Cavener consensus sequence with elements from the Malacosoma neustria nucleopolyhedrovirus (MnNPV) polyhedrin gene has been inserted between the promoter and EGFP sequences. The 3' UTR (666bp) from the Autographa californica nucleopolyhedrovirus (AcNPV) p10 gene is present downstream of the EGFP sequences.

(Pfeiffer et al., 2012)

UASt regulatory sequences (comprises 10xUAS sites plus Hsp70 sequence that includes the promoter and 203bp of 5'UTR sequence) drive expression of EGFP.

(DeLuca and Spradling, 2018)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (11)
Reported As
Symbol Synonym
10XUAS-GFP
(Kaufman et al., 2020)
10XUAS-IVS-Syn21-GFP-p10
(Karkali and Martin-Blanco, 2021, Suver et al., 2016, Pfeiffer et al., 2012)
10xUAS-IVS-syn21-GFP-p10
(Jung et al., 2020)
10xUAS::IVS-Syn21-GFP-p10
(Matinyan et al., 2021)
JFRC81-10XUAS-IVS-Syn21-GFP-p10
(Kaufman et al., 2020, Xie et al., 2018)
P{10XUAS-IVS-Syn21-GFP-p10}
(FlyBase, 1992-)
P{10xUAS-IVS-Syn21-GFP-p10}
(Garner et al., 2018)
UAS-GFP
(Garner et al., 2018)
UAStGFP
(DeLuca and Spradling, 2018)
pJFRC81
(Ho et al., 2022, DeLuca and Spradling, 2018, Pfeiffer et al., 2012)
pJFRC81-10xUAS-IVS-Syn21-GFP-p10
(Wohl et al., 2023, Chiu et al., 2021)
Secondary FlyBase IDs
    References (13)