FB2024_03 , released June 25, 2024
Recombinant Construct: P{UAS-AKAR2}
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General Information
Symbol
P{UAS-AKAR2}
FlyBase ID
FBtp0093261
Feature type
transgenic_transposable_element
Component Allele(s)
Avic\GFPAKAR2.UAS
Also Known As
UAS-AKAR2
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Avic\GFPAKAR2.UAS
Transgenic product class
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Avic\GFP
Description

UASt regulatory sequences drive expression of the genetically encoded A-kinase activity reporter "AKAR2" (see PMID:16177793). This protein consists of two fluorescent proteins (ECFP and Citrine) that are separated by a phosphothreonine-binding domain (forkhead associated domain 1 (FHA1) from the Saccharomyces cerevisiae RAD53 gene) and an optimised protein kinase A (PKA) substrate domain (LRRATLVD). When phosphorylated by PKA, the PKA substrate domain interacts with the binding pocket of the FHA1 domain, increasing the fluorescence resonance energy transfer (FRET) between the two fluorophores.

(Gervasi et al., 2010)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (2)
Stocks containing insertion(s) of this recombinant construct
Bloomington
80957
w1118; P{UAS-AKAR2}2
80958
w1118; P{UAS-AKAR2}3
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
AKAR2
(Turrel et al., 2020)
P{UAS-AKAR2}
(FlyBase, 1992-)
UAS-AKAR2
(Scheunemann et al., 2019, Scheunemann et al., 2018, Gervasi et al., 2010)
Secondary FlyBase IDs
    References (6)