Ten copies of the UAS regulatory sequence are separated from a non-fluorescent 'spaghetti monster GFP' ('smGdP') marker by an FRT cassette containing stop sequences, which prevents expression of the marker in the absence of FLP recombinase. The smGdP marker consists of 10 copies of the Tag:V5 tag inserted in groups into the backbone of the superfolder green fluorescent protein, which has been rendered non-fluorescent by mutation. A N-terminal myristoylation signal (Tag:Myr(Src64B)) is also present and the marker has been optimised for Drosophila codon usage.
Ten copies of the UAS regulatory sequence are separated from a non-fluorescent 'spaghetti monster GFP' ('smGdP') marker by an FRT cassette containing stop sequences, which prevents expression of the marker in the absence of FLP recombinase. The smGdP marker consists of 10 copies of the Tag:FLAG tag inserted in groups into the backbone of the superfolder green fluorescent protein, which has been rendered non-fluorescent by mutation. A N-terminal myristoylation signal (Tag:Myr(Src64B)) is also present and the marker has been optimised for Drosophila codon usage.
Tandem vector containing two differently tagged non-fluorescent 'spaghetti monster GFP' ('smGdP') markers separated by a 2.8kb gypsy-insulated spacer. One marker (Avic\GFP10xScer\UAS.Scer\FRT.smGdP.T:Myr-Src64B,T:SV5\V5 is tagged with T:SV5\V5 and the other (Avic\GFP10xScer\UAS.Scer\FRT.smGdP.T:Myr-Src64B,T:Zzzz\FLAG) is tagged with T:Zzzz\FLAG. In each case the smGdP marker is separated from its regulatory sequence by a Scer\FRT cassette containing stop sequences, preventing expression of either marker in the absence of FLPase.