FB2024_03 , released June 25, 2024
Recombinant Construct: PBac{20XUAS-IVS-Syn21-shits1-p10}
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General Information
Symbol
PBac{20XUAS-IVS-Syn21-shits1-p10}
FlyBase ID
FBtp0115095
Feature type
transgenic_transposable_element
Component Allele(s)
shits1.IVS.Syn21.p10.20xUAS
Also Known As
UAS-shibirets, pJFRC99-20XUAS-IVS-Syn21-Shibire-ts1-p10
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele shits1.IVS.Syn21.p10.20xUAS
Transgenic product class
dominant_negative_variant
(Pfeiffer et al., 2012)
missense_variant
(Pfeiffer et al., 2012)
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
shi
Description

20 copies of the UAS site are fused upstream of the shi1 coding region (this encodes a temperature-sensitive dominant negative form of shi). A small intron derived from the Mhc gene followed by a synthetic AT-rich 21bp sequence made by combining the Cavener consensus sequence with elements from the Malacosoma neustria nucleopolyhedrovirus (MnNPV) polyhedrin gene has been inserted between the promoter and coding sequences. The 3' UTR (666bp) from the Autographa californica nucleopolyhedrovirus (AcNPV) p10 gene is present downstream of the coding sequence.

(Pfeiffer et al., 2012)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (9)
Reported As
Symbol Synonym
20XUAS-IVS-Syn21-Shibirets1-p10
(Pfeiffer et al., 2012)
20xShibire(ts)
(Chia and Scott, 2020)
20xUAS-IVS-Syn21-Shits
(Hobin et al., 2022)
JFRC99-20XUAS-IVS-Syn21-Shibire-ts1-p10
(Strother et al., 2018)
PBac{20XUAS-IVS-Syn21-shits1-p10}
(FlyBase, 1992-)
UAS-shibirets
(Lyu et al., 2022, Green et al., 2017)
pJFRC99
(Pfeiffer et al., 2012)
pJFRC99-20XUAS-IVS-Syn21- Shibirets1 -p10
(Strother et al., 2017)
pJFRC99-20XUAS-IVS-Syn21-Shibire-ts1-p10
(Lyu et al., 2022, Green et al., 2017)
Secondary FlyBase IDs
    References (8)