FB2024_02 , released April 23, 2024
Recombinant Construct: TI{20XUAS-SPARC2-S-Syn21-CsChrimson::tdTomato-3.1}
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General Information
Symbol
TI{20XUAS-SPARC2-S-Syn21-CsChrimson::tdTomato-3.1}
FlyBase ID
FBtp0138025
Feature type
engineered_region
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Construct components
Transgenic product class
Component
Use(s)
Description

20XUAS regulatory sequences are separated from sequence encoding tdTomato-tagged CsChrimson by a "SPARC2-S" module that comprises: an attP site of 34bp, a STOP cassette, a second attP site (60bp, full-length site), ribozyme sequence (to prevent leaky expression in the absence of phiC31:int) and an attB site. phiC31:int catalyzes irreversible recombination between the attB site and one of the two attP sites; if the attP site upstream of the STOP cassette is utilized, the STOP cassette is excised, permitting expression of tdTomato-tagged CsChrimson, while if the attP site downstream of the STOP cassette is utilized, the STOP cassette is preserved. The "SPARC2-S" module typically results in expression of the effector (in this case tdTomato-tagged CsChrimson) in approximately 5% of cells (recombination of the attB site with the full-length attP downstream of the STOP cassette is more likely than recombination with the shortened attP site upstream of the STOP cassette).

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
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Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
SPARC-S-CsChrimson::tdTomato
SPARC2-S-CsChrimson::tdTomato
TI{20XUAS-SPARC2-S-Syn21-CsChrimson::tdTomato-3.1}
Secondary FlyBase IDs
    References (5)