FB2024_03 , released June 25, 2024
Recombinant Construct: P{UAS-FH-Ras85D.Y4F}
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General Information
Symbol
P{UAS-FH-Ras85D.Y4F}
FlyBase ID
FBtp0144073
Feature type
transgenic_transposable_element
Component Allele(s)
Ras85DY4F.UAS.Tag:FLAG,Tag:polyHis
Also Known As
UAS Flag-His6-RasY4F
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Ras85DY4F.UAS.Tag:FLAG,Tag:polyHis
Transgenic product class
missense_variant
(Washington et al., 2020)
polypeptide_post_translational_processing_variant
(Washington et al., 2020)
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Ras85D
Tagged with
Tag:FLAG
Tag:polyHis
Description

UASt regulatory sequences drive expression of Ras85D carrying the amino acid replacement Y4F (non-phosphorylatable mutation). The coding sequence is tagged at the N-terminal end with Tag:FLAG and Tag:polyHis.

(Washington et al., 2020)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
P{UAS-FH-Ras85D.Y4F}
(FlyBase, 1992-)
UAS FLAG-His6-RasY4F
(Karunaraj et al., 2024)
UAS Flag-His6-RasY4F
(Washington et al., 2020)
UAS RasY4F
(Karunaraj et al., 2024)
Secondary FlyBase IDs
    References (3)