FB2024_03 , released June 25, 2024
Recombinant Construct: P{3xUAS-IVS-Syt1::smGdP-HA}
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General Information
Symbol
P{3xUAS-IVS-Syt1::smGdP-HA}
FlyBase ID
FBtp0145743
Feature type
transgenic_transposable_element
Component Allele(s)
Syt13xUAS.smGdP.Tag:HA
Also Known As
pJFRC51-3XUAS-IVS-Syt::smHA, 3xUAS-Syt::smGFP-HA
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Syt13xUAS.smGdP.Tag:HA
Transgenic product class
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Syt1
Tagged with
Tag:HA
Description

Three copies of the UAS regulatory sequence drive expression of the Syt1 coding sequence which is tagged at the C-terminal end with a non-fluorescent 'spaghetti monster GFP' ('smGdP') marker which consists of 10 copies of the Tag:HA tag inserted in groups into the backbone of a superfolder green fluorescent protein which has been rendered non-fluorescent by mutation. The smGdP marker has been optimised for Drosophila codon usage.

(Nern et al., 2015)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (12)
Reported As
Symbol Synonym
3XUAS-Syt-sm-HA
(Certel et al., 2022)
3xUAS-Syt::smGFP-HA
(Sterne et al., 2021, Wang et al., 2021, Dolan et al., 2019)
P{3xUAS-IVS-Syt1::smGdP-HA}
(FlyBase, 1996-, FlyBase, 1992-)
P{3xUAS-Syt1::smGFP-HA}
(FlyBase, 1992-)
Syt-smHA
(Certel et al., 2022)
Syt::smGFP-HA
(Aso et al., 2014)
UAS-syt-HA
(Wang et al., 2021)
pJFRC51-3XUAS-IVS-Syt::smGFP::HA
(Seidner et al., 2015)
pJFRC51-3XUAS-IVS-Syt::smHA
(Davis et al., 2020, Meissner et al., 2018)
pJFRC51-3XUAS-IVS-syt::smHA
(Baker et al., 2022, Morimoto et al., 2020, Wu et al., 2016)
pJFRC51-3xUAS-IVS-Syt::smGFP-HA
(Nern et al., 2015)
pJFRC51-3xUAS-Syt::smGFP-HA
(Aso et al., 2014)
Secondary FlyBase IDs
  • FBtp0108755
  • FBtp0099448
References (14)