FB2024_03 , released June 25, 2024
Recombinant Construct: P{U6-4gRNAs(mCD8)}
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General Information
Symbol
P{U6-4gRNAs(mCD8)}
FlyBase ID
FBtp0159144
Feature type
transgenic_transposable_element
Component Allele(s)
Mmus\Cd8asnRNA:U6:96Ac.4gRNAs
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Construct components
Component allele Mmus\Cd8asnRNA:U6:96Ac.4gRNAs
Transgenic product class
sgRNA
(Chen et al., 2020)
Component
Use(s)
Regulatory region(s)
snRNA:U6:96Ac
Encoded product / tool
Mmus\Cd8a
Description

The snRNA:U6:96Ac promoter is separated from four gRNAs (each with an attP site upstream of the gRNA) by an attB site and a Disc\RFP3xP3.PB marker. In the absence of phiC31:int, the Disc\RFP3xP3.PB marker prevents expression of the gRNAs. In the presence of phiC31:int, the attB site can recombine stochastically with any of the four attP sites upstream of a gRNA, removing the marker, creating an attR site and allowing expression of a single gRNA (the one immediately downstream of the attR site). Each of the gRNAs targets a different region of the Tag:M(mCd8a) tag.

(Chen et al., 2020)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{U6-4gRNAs(mCD8)}
(FlyBase, 1992-)
pCis-{4gRNAs-mCD8}
(Chen et al., 2020)
Secondary FlyBase IDs
    References (2)