FB2024_04 , released June 25, 2024
Recombinant Construct: P{bamP(198)-CFI}
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General Information
Symbol
P{bamP(198)-CFI}
FlyBase ID
FBtp0159166
Feature type
transgenic_transposable_element
Component Allele(s)
Scer\FLP1bam.198.2A-CFI
Scer\SCEIbam.198.2A-CFI
Spyo\Cas9bam.198.2A-CFI
Also Known As
bamP(198)-Cas9-P2A-FLP-E2A-I-SceI
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Spyo\Cas9bam.198.2A-CFI
Transgenic product class
Component
Use(s)
Regulatory region(s)
bam
Encoded product / tool
Cas9
Tagged with
Tag:NLS(SV40-largeT)
Description

A female cystoblast-specific bam promoter fragment drives expression of a multicistronic transcript that encodes three open reading frames, each separated by a 2A linker (this results in separate translation of each protein). The encoded proteins are Cas9 (tagged at the N-terminal end with Tag:NLS(SV40-largeT)), FLP and I-SceI.

(Chen et al., 2015)
Component allele Scer\FLP1bam.198.2A-CFI
Transgenic product class
Component
Use(s)
Regulatory region(s)
bam
Encoded product / tool
FLP
Description

A female cystoblast-specific bam promoter fragment drives expression of a multicistronic transcript that encodes three open reading frames, each separated by a 2A linker (this results in separate translation of each protein). The encoded proteins are Cas9 (tagged at the N-terminal end with Tag:NLS(SV40-largeT)), FLP and I-SceI.

(Chen et al., 2015)
Component allele Scer\SCEIbam.198.2A-CFI
Transgenic product class
Component
Use(s)
Regulatory region(s)
bam
Encoded product / tool
I-SceI
Description

A female cystoblast-specific bam promoter fragment drives expression of a multicistronic transcript that encodes three open reading frames, each separated by a 2A linker (this results in separate translation of each protein). The encoded proteins are Cas9 (tagged at the N-terminal end with Tag:NLS(SV40-largeT)), FLP and I-SceI.

(Chen et al., 2015)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
P{bamP(198)-CFI}
(FlyBase, 1992-)
bam198-CFI
(Chen et al., 2015)
bamP(198)-Cas9-P2A-FLP-E2A-I-SceI
(Chen et al., 2020, Chen et al., 2015)
bamP(198)-Cas9:2A:FLP:2A:I-SceI
(Chen et al., 2020)
bamP-Cas9-2A-FLP-2A-I-SceI
(Chen et al., 2015)
Secondary FlyBase IDs
    References (3)