A bam promoter fragment drives expression of a multicistronic transcript that encodes two open reading frames, each separated by a 2A linker (this results in separate translation of each protein). The encoded proteins are Cas9 and FLP.
A bam promoter fragment drives expression of a multicistronic transcript that encodes two open reading frames, each separated by a 2A linker (this results in separate translation of each protein). The encoded proteins are Cas9 and FLP.