FlyBase Allele Report: Dmel\BicD[R26]

General Information

Symbol

Dmel\BicD^R26

Species

D. melanogaster

Name

FlyBase ID

FBal0001144

Feature type

allele

Associated gene

Dmel\BicD

Associated Insertion(s)

Carried in Construct

Also Known As

Bic-D^R26

Key Links

Genomic Maps

JBrowse

Allele class

antimorphic allele - genetic evidence

loss of function allele

hypomorphic allele - genetic evidence

Mutagen

X ray

Nature of the Allele

Allele class

antimorphic allele - genetic evidence

(Oh et al., 2000)

hypomorphic allele - genetic evidence

(Huynh and St. Johnston, 2000, Capri et al., 1997, Swan and Suter, 1996, Lantz et al., 1994)

loss of function allele

(Digilio et al., 1996, Lantz et al., 1992, Suter and Steward, 1991)

Mutagen

X ray

(Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

BicD¹

(Oh et al., 2000, Suter and Steward, 1991)

Cytology

Description

Deletion of amino acids 376-379.

(Oh et al., 2000)

Deletion of 12 nucleotides.

(Suter and Steward, 1991)

sequenced by Suter and Steward, 1991 deletion of residues 376-379 superimposed on BicD¹ lesion

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:17,463,478..17,463,489 [-]

Comment:

A deletion of 12 nucleotides which removes amino acids 376-379. This is in addition to the F684I mutation of the parental BicD[1] chromosome.

(Suter and Steward, 1991)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage & oogenesis

oocyte

(Suter and Steward, 1991)

cyst cell of ovary

(Suter and Steward, 1991)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage & oogenesis

nurse cell

(Suter and Steward, 1991)

germarium region 1

(Suter and Steward, 1991)

Additional Information

Statement

Reference

BicD^R26 protein becomes localized to the oocyte at significantly higher levels than in wild type.

(Suter and Steward, 1991)

In wild type females, BicD RNA is detected very early in oogenesis in the 16 cystocyte cluster and progressively accumulates in a single cell, the oocyte. In BicD^R26 mutants, BicD RNA does not localized to a single cystocyte, but is found in all of the pro-nurse cells.

(Suter and Steward, 1991)

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile

(Sass et al., 1995)

female sterile | recessive

(Lantz et al., 1994, Bohrmann et al., 1992, Lantz et al., 1992)

Phenotype Manifest In

dorsal appendage

(Swan and Suter, 1996)

dorsal appendage, with BicD^H2

(Oh et al., 2000)

dorsal appendage, with BicD^H3

(Oh et al., 2000)

dorsal appendage, with BicD^HA40.Tag:HA

(Oh et al., 2000)

egg, with BicD^H2

(Oh et al., 2000)

egg, with BicD^H3

(Oh et al., 2000)

egg, with BicD^HA40.Tag:HA

(Oh et al., 2000)

egg chamber

(Capri et al., 1997, Lantz et al., 1992)

egg chorion, with BicD^H2

(Oh et al., 2000)

egg chorion, with BicD^H3

(Oh et al., 2000)

egg chorion, with BicD^HA40.Tag:HA

(Oh et al., 2000)

embryonic neuroblast, with BicD^HA40.Tag:HA, Df(2L)TW119

(Hughes et al., 2004)

embryonic neuroblast (with Df(2L)TW119), with BicD^HA40.Tag:HA

(Hughes et al., 2004)

embryonic neuroblast & spindle, with BicD^HA40.Tag:HA, Df(2L)TW119

(Hughes et al., 2004)

embryonic neuroblast & spindle (with Df(2L)TW119), with BicD^HA40.Tag:HA

(Hughes et al., 2004)

germline cyst

(Huynh and St. Johnston, 2000, Theurkauf et al., 1993)

oocyte

(Masrouha et al., 2003, Huynh and St. Johnston, 2000, Knowles and Cooley, 1994, Lantz et al., 1994, Bohrmann et al., 1992, Lantz et al., 1992)

ovary

(Digilio et al., 1996)

synaptonemal complex

(Huynh and St. Johnston, 2000)

synaptonemal complex | ectopic

(Huynh and St. Johnston, 2000)

Detailed Description

Statement

Reference

13% of neuroblast mitoses are more than 45^o off-axis in embryos derived from BicD^{HA40.T:Ivir\HA1} ; BicD^R26/Df(2L)TW119 females mated to BicD^R26/+ males (in contrast to wild type where all neuroblast divisions are oriented within 45^o of the apicobasal axis). The average length of metaphase spindles in these mutant neuroblasts is significantly less than normal.

(Hughes et al., 2004)

Mutant ovaries fail to make an oocyte.

(Masrouha et al., 2003)

More than 4 cells per germline cyst enter meiosis in homozygous females, but the cysts still retain a graded distribution of the synaptonemal complex (SC), with the highest levels in the two pro-oocytes. The SC sometimes becomes restricted to these 2 cells in region 2b/3, but eventually disappears in region 3. The 2 cells eventually adopt the nurse cell fate.

(Huynh and St. Johnston, 2000)

When in combination with BicD^H2, BicD^H3 or BicD^{HA40.T:Ivir\HA1} over null or loss of function BicD, BicD^R26 increases the proportion of ventralised eggs to 100%. When transheterozygous with a wild type allele, female fertility is normal.

(Oh et al., 2000)

Fusomes in the cysts of BicD^r/BicD^R26 ovaries appear to form normally.

(de Cuevas and Spradling, 1998)

Egg chambers of hemizygous females start to degenerate around stage 7.

(Capri et al., 1997)

Embryos exhibit a fused dorsal appendage.

(Swan and Suter, 1996)

No oocyte differentiation, no microtubule organising centre.

(Knowles and Cooley, 1994)

Fusomes are normal.

(Lin et al., 1994)

No single microtubule organizing center forms in the germaria of hemizygous females, instead several small foci are present. The actin cytoskeleton is unaffected.

(Theurkauf et al., 1993)

Abdominal defect: no eggs.

(Ephrussi et al., 1991)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

BicD^R26 has phenotype, suppressible by kst⁰¹³¹⁸

(Nguyen and Suter, 1997)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Revertant.

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

Bic-D^DR26

(Ephrussi et al., 1991)

Bic-D^R26

(Oh and Steward, 2001, Oh et al., 2000, de Cuevas and Spradling, 1998, Capri et al., 1997, Nguyen and Suter, 1997, Digilio et al., 1996, Swan and Suter, 1996, Baksa and Steward, 1995, Micklem, 1995, Sass et al., 1995, Knowles and Cooley, 1994, Theurkauf et al., 1993, Lantz et al., 1992)

Bic-D^r26

(Lin et al., 1994)

BicD^7134r26

(Tearle and Nusslein-Volhard, 1987)

BicD^R26

(Jagut et al., 2013, Bullock et al., 2006, Bullock et al., 2006, Delanoue and Davis, 2005)

BicD^r26

(Mach and Lehmann, 1997)

BicD^rv1

(Bohrmann et al., 1992)

Name Synonyms

Secondary FlyBase IDs

FBal0001143

References (38)

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