FlyBase Allele Report: Dmel\Cat[n1]

General Information

Symbol

Dmel\Catⁿ¹

Species

D. melanogaster

Name

FlyBase ID

FBal0001548

Feature type

allele

Associated gene

Dmel\Cat

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Park et al., 2004, Mackay and Bewley, 1989)

Mutagen

ethyl methanesulfonate

Progenitor genotype

Cytology

Description

Single amino acid replacement in the haem domain.

(Wheeler et al., 1995)

Amino acid replacement: E189K. Nucleotide substitution: G?A.

(Griswold et al., 1993)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:18,826,845..18,826,845 [+]

Nucleotide change:

G18826845A

Reported nucleotide change:

G?A

Amino acid change:

E189K | Cat-PA

Reported amino acid change:

E189K

(Griswold et al., 1993)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Alexander disease

modeled by Hsap\GFAP^R79H.UAS

(Wang et al., 2011)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult midgut

(Choi et al., 2008)

enterocyte

(Choi et al., 2008)

enteroendocrine cell | increased number

(Choi et al., 2008)

intestinal stem cell

(Choi et al., 2008)

intestinal stem cell of posterior adult midgut epithelium

(Park et al., 2015)

Detailed Description

Statement

Reference

Catⁿ¹ mutants show a significant increase in DNA damage in adult midgut progenitor cells (esg-positive intestinal stem cells and enteroblasts), assessed by pS/TQ immunofluorescence, as compared to controls.

(Park et al., 2015)

Catⁿ¹ heterozygotes show increased mortality when exposed to ethanol during development compared to controls.

(Logan-Garbisch et al., 2014)

Catⁿ¹ homozygous adult males exhibit a severe decrease in lifespan under normal or chronic hypoxic conditions, as compared to controls.

(Wicks et al., 2009)

Reactive oxygen species generation and BrdU incorporation in the midguts of mutant flies is significantly higher than in wild-type flies.

The intestinal stem cell populations in the midgut of mutant flies is increased compared to controls. The number of enteroendocrine cells is increased by 2.2 fold, while the number of differentiated enterocytes with large nuclei is reduced by 0.7 fold in the mutant adult midgut.

(Choi et al., 2008)

Only about 10% of homozygous Catⁿ¹ flies eclose from their pupal cases. Homozygous Catⁿ¹ mutants also show a reduced lifespan, living half as long as wild type flies.

(Missirlis et al., 2003)

Catⁿ¹/Catⁿ⁴ transheterozygotes are very sensitive to H₂O₂. Sensitivity is considerably attenuated in the presence of a single copy of P{Cat7.0}.

(Orr et al., 1996)

Catalase activity reduced.

(Harshman et al., 1991)

Exhibit gene dosage effect for catalase activity as a heterozygote. H₂O₂ hypersensitive phenotype.

(Mackay and Bewley, 1989)

CRM negative.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Catⁿ¹ has short lived phenotype, non-suppressible by Gtpx^lacZ

(Missirlis et al., 2003)

Enhancer of

Statement

Reference

Catⁿ¹/Cat[+] is an enhancer of increased cell death | progressive phenotype of Hsap\GFAP^UAS.cWa, Scer\GAL4^repo

(Wang et al., 2011)

Catⁿ¹/Cat[+] is an enhancer of paralytic phenotype of Hsap\GFAP^UAS.cWa, Scer\GAL4^repo

(Wang et al., 2011)

Catⁿ¹/Cat[+] is an enhancer of lethal | recessive phenotype of Trxr1^G0481

(Missirlis et al., 2001)

Phenotype Manifest In

Enhancer of

Statement

Reference

Catⁿ¹/Cat[+] is an enhancer of lamina phenotype of Hsap\GFAP^UAS.cWa, Scer\GAL4^repo

(Wang et al., 2011)

Catⁿ¹/Cat[+] is an enhancer of adult brain phenotype of Hsap\GFAP^UAS.cWa, Scer\GAL4^repo

(Wang et al., 2011)

Catⁿ¹/Cat[+] is an enhancer of neuron | cell non-autonomous phenotype of Hsap\GFAP^UAS.cWa, Scer\GAL4^repo

(Wang et al., 2011)

Suppressor of

Statement

Reference

Catⁿ¹ is a suppressor of eye phenotype of Raf::tor^12D.sev

(Morey et al., 2001)

Catⁿ¹ is a suppressor of photoreceptor cell R7 | ectopic phenotype of Raf::tor^12D.sev

(Morey et al., 2001)

Catⁿ¹ is a suppressor of photoreceptor cell R7 phenotype of Raf::tor^12D.sev

(Morey et al., 2001)

Catⁿ¹ is a suppressor of eye phenotype of sev^S11.Tag:MYC

(Morey et al., 2001)

Catⁿ¹ is a suppressor of ommatidium phenotype of sev^S11.Tag:MYC

(Morey et al., 2001)

Catⁿ¹ is a suppressor of photoreceptor cell R7 | ectopic phenotype of sev^S11.Tag:MYC

(Morey et al., 2001)

Catⁿ¹ is a suppressor of photoreceptor neuron phenotype of sev^S11.Tag:MYC

(Morey et al., 2001)

NOT Suppressor of

Statement

Reference

Catⁿ¹ is a non-suppressor of eye phenotype of Ras64B^V12.sev

(Morey et al., 2001)

Catⁿ¹ is a non-suppressor of photoreceptor neuron phenotype of Ras64B^V12.sev

(Morey et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

A Catⁿ¹/+ background suppresses the rough eye phenotype observed when Dref^Scer\UAS.cSa is expressed in the developing eye under the control of Scer\GAL4^GMR.PS.

(Park et al., 2004)

Expression of either one or two copies of PHGPx^T:Ecol\lacZ is unable to suppress the reduction in lifespan seen in Catⁿ¹ mutant flies.

(Missirlis et al., 2003)

Xenogenetic Interactions

Statement

Reference

A Catⁿ¹ heterozygous background significantly enhances Hsap\GFAP^Scer\UAS.cWa toxicity and increases the frequency of mechanically-stimulated seizures in these flies. An increase in cellular toxicity does not alter the number of inclusion bodies but does increase autophagy induction.

A Catⁿ¹ heterozygous background significantly enhances Hsap\GFAP^{R79H.Scer\UAS} toxicity and increases the frequency of mechanically-stimulated seizures in these flies. An increase in cellular toxicity does not alter the number of inclusion bodies but does increase autophagy induction.

(Wang et al., 2011)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

4014

Catⁿ¹ cp¹ in¹ kni^ri-1 p^p/TM3, Sb¹ Ser¹

Kyoto

107554

Catⁿ¹ / TM3, Sb¹ Ser¹

Notes on Origin

Discoverer

Comments

In Catⁿ¹ eggs of L.boulardi are melanotically encapsulated but, in contrast to the wild-type, survive.

(Nappi et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Cat^N1

(Wheeler et al., 1995)

Catⁿ¹

(Park and Kim, 2020, Pyo et al., 2018, Park et al., 2015, Logan-Garbisch et al., 2014, Wang et al., 2011, Choi et al., 2008, Park et al., 2004, Missirlis et al., 2003)

catⁿ¹

(Chew et al., 2021, Wicks et al., 2009, Missirlis et al., 2001)

Name Synonyms

Secondary FlyBase IDs

References (18)

Report Sections

Open Close