FlyBase Allele Report: Dmel\dor[4]

General Information

Symbol

Dmel\dor⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0002732

Feature type

allele

Associated gene

Dmel\dor

Associated Insertion(s)

Carried in Construct

Also Known As

dor^66g

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Lefevre, 1970)

Progenitor genotype

Cytology

Polytene chromosomes normal.

(Lefevre, 1970)

Description

Frameshift after amino acid residue 993, substituting 30 new residues for the terminal 9 amino acids.

(Sevrioukov et al., 1999)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

sequence_alteration

X:1,668,338..1,668,340 [+]

Comment:

frameshift after this amino acid (993) results in the substitution of 30 new residues in place of the last 9 amino acids; exact location/nature of mutation unspecified.

(Sevrioukov et al., 1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell death | oogenesis stage S14

(Bass et al., 2009)

abnormal eye color

(Sevrioukov et al., 1999)

decreased cell death

(Yacobi-Sharon et al., 2013)

female semi-fertile

(Lefevre, 1970)

male fertile

short lived | male

(Simonsen et al., 2007)

viable

Phenotype Manifest In

Detailed Description

Statement

Reference

dor⁴ mutant males exhibit ~60% less spermatogonial cyst death compared to controls.

(Yacobi-Sharon et al., 2013)

dor⁴ male wandering third instar larvae show defects in morphology of LysoTracker-positive puncta and lipid droplets during ecdysone-induced autophagy.

(Bartlett et al., 2011)

92% of dor⁴ stage 14 egg chambers show persisting nurse cell nuclei, compared to 7% in controls.

(Bass et al., 2009)

Mutant males have a shortened adult life span compared to controls, with a mean life span of 13.94 days at 25^o.

(Simonsen et al., 2007)

Hemocyte endosomal degradation is significantly reduced in dor⁴ mutants compared to wild-type cells. This reduction also correlates with an increased severity of eye-colour defect. This reduction also correlates with an increased severity of eye-colour defect (with increased severity in dor¹ compared to dor⁴. Distinct from cells in wild-type and car¹ animals, the large endosomes in mutant dor⁴ alleles appear blocked in progression to later stages.

(Sriram et al., 2003)

Synapse size, along with bouton number, is decreased in dor⁴ mutants.

(Narayanan et al., 2000)

Eye colour: orange.

(Sevrioukov et al., 1999)

Eye colour: dark orange at 25^oC.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

dor⁴ has lethal | pupal stage phenotype, enhanceable by hook¹¹

(Narayanan et al., 2000)

NOT Enhanced by

Statement

Reference

dor⁴ has paralytic phenotype, non-enhanceable by shi²

(Narayanan et al., 2000)

Enhancer of

Statement

Reference

dor[+]/dor⁴ is an enhancer of visible phenotype of Scer\GAL4^GMR.PF, bchs^EP2299

(Simonsen et al., 2007)

dor⁴ is an enhancer of lethal | pupal stage phenotype of hook¹¹

(Narayanan et al., 2000)

Suppressor of

Statement

Reference

dor[+]/dor⁴ is a suppressor of increased cell death phenotype of Hsc70-5^KK100233, Scer\GAL4^NP5130

(Banerjee and Chinthapalli, 2014)

dor⁴ is a suppressor of abnormal neuroanatomy phenotype of hook¹¹

(Narayanan et al., 2000)

Phenotype Manifest In

Enhancer of

Statement

Reference

dor[+]/dor⁴ is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, bchs^EP2299

(Simonsen et al., 2007)

Suppressor of

Statement

Reference

dor[+]/dor⁴ is a suppressor of adult midgut progenitor cell phenotype of Hsc70-5^KK100233, Scer\GAL4^NP5130

(Banerjee and Chinthapalli, 2014)

dor⁴ is a suppressor of synapse phenotype of hook¹¹

(Narayanan et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Less than 5% of dor⁴ hk¹¹ double mutants eclose into adults, with the lethal phase occurring in the late pupal stage. dor⁴ hk¹¹ double mutants have a decreased number of boutons and are indistinguishable from dor⁴ mutants alone. However, in the presence of a dor duplication, such as Dp(1;Y)1E, the size of the dor⁴ hk¹¹ double mutant synapse is like hk¹¹ mutants.

(Narayanan et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

dor⁸

(Narayanan et al., 2000)

Rescued by

dor⁴ is rescued by dor^1-1002.UAS/Scer\GAL4^GMR.PF

(Sevrioukov et al., 1999)

dor⁴ is rescued by dor^400-1002.UAS/Scer\GAL4^GMR.PF

(Sevrioukov et al., 1999)

Not rescued by

dor⁴ is not rescued by dor^{Δ401-800.UAS}/Scer\GAL4^GMR.PF

(Sevrioukov et al., 1999)

dor⁴ is not rescued by dor^1-802.UAS/Scer\GAL4^GMR.PF

(Sevrioukov et al., 1999)

Comments

The defect in degradation in the dor⁴ mutant cells is completely rescued by Dp(1;Y)1E.

(Sriram et al., 2003)

The dor⁴/dor⁸ transheterozygote exhibits the same phenotype as the dor⁴ homozygote.

(Narayanan et al., 2000)

The eye colour phenotype of dor⁴ flies is rescued by dor^{1-1002.Scer\UAS} or dor^{400-1002.Scer\UAS} expressed under the control of Scer\GAL4^GMR.PF. The eye colour phenotype of dor⁴ flies is not rescued by dor^{Δ401-800.Scer\UAS} or dor^{1-802.Scer\UAS} expressed under the control of Scer\GAL4^GMR.PF, and the level of eye pigmentation in these flies is further reduced compared to dor⁴ single mutant flies.

(Sevrioukov et al., 1999)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

dor⁴/C(1)RM, y¹ w¹ f¹

Kyoto

105692

dor⁴/C(1)RM, y¹ w¹ f¹

Notes on Origin

Discoverer

Lefevre.

G. Lefevre, July 1966.

(Lefevre, 1970)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

dor⁴

(Walsh et al., 2021, Shimizu et al., 2017, Timmons et al., 2016, Banerjee and Chinthapalli, 2014, Yacobi-Sharon et al., 2013, Wong et al., 2012, Bartlett et al., 2011, Popodi et al., 2010-, Bass et al., 2009, Pulipparacharuvil et al., 2005)

dor^66g

(Belyaeva et al., 1980, Lefevre, 1970)

Name Synonyms

deep orange4

(Simonsen et al., 2007)

Secondary FlyBase IDs

References (16)

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