FB2024_03 , released June 25, 2024
Allele: Dmel\Egfrf1
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General Information
Symbol
Dmel\Egfrf1
Species
D. melanogaster
Name
FlyBase ID
FBal0003529
Feature type
allele
Associated gene
Dmel\Egfr
Associated Insertion(s)
Carried in Construct
Also Known As
flb1F26, flbIF26, Egfr1F26
Key Links
Genomic Maps

Allele class

heat sensitive loss of function allele

temperature conditional amorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

heat sensitive loss of function allele

(Clifford and Schupbach, 1992)

temperature conditional amorphic allele - genetic evidence

(Raz and Shilo, 1992)
Mutagen
ethyl methanesulfonate
(Clifford and Schupbach, 1994, Clifford and Schupbach, 1989, Tearle and Nusslein-Volhard, 1987)
Progenitor genotype
Cytology
Description

Nucleotide substitution: C3844T. Amino acid replacement: P1113L.

(Clifford and Schupbach, 1994)

Amino acid replacement: P1112L.

(Raz and Shilo, 1992)

Nucleotide substitution: C3335T. Amino acid replacement: P1112L. (numbering relates to the type II 5' splicing alternative, FBrf0043895). This amino acid residue is in the tyrosine kinase domain.

(Raz et al., 1991)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2R:21,558,286..21,558,286 [+]
Nucleotide change:

C21558286T

Reported nucleotide change:

C3844T

Amino acid change:

P1113L | Egfr-PA; P1162L | Egfr-PB

Reported amino acid change:

P1113L

(Clifford and Schupbach, 1994, Raz et al., 1991)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
X78920
CAA55523
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      abdominal 1 ventral denticle belt

      (Raz and Shilo, 1993)

      abdominal 2 ventral denticle belt

      (Raz and Shilo, 1993)

      abdominal 3 ventral denticle belt

      (Raz and Shilo, 1993)

      abdominal 4 ventral denticle belt

      (Raz and Shilo, 1993)

      abdominal 5 ventral denticle belt

      (Raz and Shilo, 1993)

      abdominal 6 ventral denticle belt

      (Raz and Shilo, 1993)

      abdominal 7 ventral denticle belt

      (Raz and Shilo, 1993)

      amnioserosa

      (Shen et al., 2013)

      Bolwig organ | heat sensitive

      (Dumstrei et al., 2002)

      embryo

      (Lanoue and Jacobs, 1999)

      embryonic/larval cuticle

      (Szuts et al., 1997)

      embryonic/larval cuticle | temperature conditional

      (Shen et al., 2013)

      embryonic/larval midgut | embryonic stage

      (Szuts et al., 1998)

      embryonic/larval muscle system

      (Yarnitzky et al., 1997)

      embryonic/larval somatic muscle cell

      (Buff et al., 1998)

      embryonic central brain | heat sensitive

      (Page, 2003)

      embryonic head

      (Shen et al., 2013)

      embryonic head | heat sensitive

      (Dumstrei et al., 2002)

      embryonic midgut constriction 1

      (Szuts et al., 1998)

      embryonic midgut constriction 2

      (Szuts et al., 1998)

      embryonic midgut constriction 3

      (Szuts et al., 1998)

      embryonic neuroblast

      (Yagi et al., 1998)

      embryonic optic lobe primordium | heat sensitive

      (Dumstrei et al., 2002)

      endoderm

      (Szuts et al., 1998)

      epidermis

      (Raz and Shilo, 1992)

      eye

      (Kurada and White, 1998)

      genital disc primordium

      (Chen et al., 2005)

      head

      (Raz and Shilo, 1992)

      Keilin organ

      (Raz and Shilo, 1993)

      larval longitudinal connective

      (Raz and Shilo, 1992)

      larval RP2 motor neuron

      (Yagi et al., 1998)

      lateral cervical tract | heat sensitive

      (Page, 2003)

      midline glial cell

      (Raz and Shilo, 1992)

      mitotic domain 1 | extended germ band stage

      (Raz and Shilo, 1992)

      muscle cell of abdominal 2 dorsal acute muscle 1

      (Buff et al., 1998)

      muscle cell of abdominal 2 lateral longitudinal muscle 1

      (Buff et al., 1998)

      muscle cell of abdominal 2 ventral acute muscle 2

      (Buff et al., 1998)

      muscle cell of abdominal 3 dorsal acute muscle 1

      (Buff et al., 1998)

      muscle cell of abdominal 3 lateral longitudinal muscle 1

      (Buff et al., 1998)

      muscle cell of abdominal 3 ventral acute muscle 2

      (Buff et al., 1998)

      muscle cell of abdominal 4 dorsal acute muscle 1

      (Buff et al., 1998)

      muscle cell of abdominal 4 lateral longitudinal muscle 1

      (Buff et al., 1998)

      muscle cell of abdominal 4 ventral acute muscle 2

      (Buff et al., 1998)

      muscle cell of abdominal 5 dorsal acute muscle 1

      (Buff et al., 1998)

      muscle cell of abdominal 5 lateral longitudinal muscle 1

      (Buff et al., 1998)

      muscle cell of abdominal 5 ventral acute muscle 2

      (Buff et al., 1998)

      muscle cell of abdominal 6 dorsal acute muscle 1

      (Buff et al., 1998)

      muscle cell of abdominal 6 lateral longitudinal muscle 1

      (Buff et al., 1998)

      muscle cell of abdominal 6 ventral acute muscle 2

      (Buff et al., 1998)

      muscle cell of abdominal 7 dorsal acute muscle 1

      (Buff et al., 1998)

      muscle cell of abdominal 7 lateral longitudinal muscle 1

      (Buff et al., 1998)

      muscle cell of abdominal 7 ventral acute muscle 2

      (Buff et al., 1998)

      symmetrical commissure

      (Raz and Shilo, 1992)

      ventral denticle belt

      (Szuts et al., 1997, Rogge et al., 1995)

      ventral thoracic disc primordium

      (Kubota et al., 2000)

      wing vein L4 | heat sensitive

      (Guichard et al., 1999)
      Detailed Description
      Statement
      Reference

      Egfrf1 homozygous mutant embryos transferred from 18[o]C to 29[o]C at any stage prior to stage 10 show severe cuticle defects. Shifting slightly older (approx. stage 11) Egfrf1 mutant embryos to 29[o]C, however, results in a less severe mutant phenotype comprising a smaller head hole, distinct from other defects in the dorsal surface, and a less severe defect in germband retraction. In addition, these embryos consistently display creases or 'puckers' in the dorsal surface, which together with the mild germband retraction defect, gave them a bowed appearance. Approximately 52% of Egfrf1 embryos exhibit a bowed phenotype in a temperature shift experiment where a 2.5hr collection of embryos is aged at 18[oc for 12 hours and then shifted to 29[o]C (corresponding to later stage 10/11 at the time of the temperature shift). Of these bowed embryos, half exhibit an additional phenotype of a hole or scab in the dorsal surface. Few defects are seen in the dorsal surface of Egfrf1 embryos when they are shifted to 29[o]C at stage 12 or later.

      (Shen et al., 2013)

      In Egfrf1 stage 15 embryos, the genital disc precursor cells are completely missing.

      (Chen et al., 2005)

      When mutants are raised to the non-permissive temperature at ES 9, then a strong loss of midbrain phenotype is seen. When Egfr is removed at late ES 12, there is no gross loss of midbrain, however the circumesophageal connectives are sometimes disrupted.

      (Page, 2003)

      Heat pulses between 3 and 6 hours of development in Egfrf1 embryos result in defects in head development, but leave the visual system intact. Heat pulses at 6-8 hours of development result in non-disjunction of the Bolwig's organ and optic lobe. Heat pulses from 8-10 hours of development do not affect optic placode morphogenesis but result in a reduction in Bolwig's neurons.

      (Dumstrei et al., 2002)

      Leg disc development is severely affected when the temperature is increased to the restrictive temperature at 6 hours after egg laying (AEL). Only a mild defect is found in leg discs when embryos are shifted to the restrictive temperature at 7 hours AEL.

      (Kubota et al., 2000)

      When Egfrf1/Egfrt1 flies are raised at the non-permissive temperature (29oC)a vein-loss phenotype is seen. There is a large truncation in wing vein L4 which is similar to the Egfrt1/Deficiency phenotype. This phenotype is seen if heat shocking occurs from 6 to 18 hours APF (after puparium formation).

      (Guichard et al., 1999)

      Egfrf1 embryos grown at 25oC generates a hypomorphic, embryonic lethal phenotype.

      (Lanoue and Jacobs, 1999)

      Embryos shifted to 29oC at 6-7 hours of development fail to develop the LL1 muscle precursor. Embryos shifted to 29oC at 5-6 hours of development fail to develop the LL1, DA1 and VA2 muscle precursors.

      (Buff et al., 1998)

      Shifting mutant embryos to the restrictive temperature after germ band retraction causes abnormal midgut development.

      (Szuts et al., 1998)

      sna-positive neuroblasts are often missing in homozygous embryos. Loss of RP2 motor neurons is also seen.

      (Yagi et al., 1998)

      Homozygous embryos shifted to the restrictive temperature (25oC) at 6 hours of development give rise to larvae with residual denticle belts in their abdomens, often with 2-4 disordered rows of tapered denticles per belt. The regions of naked cuticle in between the denticle belts tend to be wider than in wild-type embryos.

      (Szuts et al., 1997)

      Embryonic muscle pattern is severely disrupted.

      (Yarnitzky et al., 1997)

      Homozygotes exhibit complete absence of ventral denticle belts.

      (Rogge et al., 1995)

      Homozygotes show a severe embryonic lethal phenotype at 29oC and a weak embryonic lethal phenotype at 18oC. Enhances the female sterility and adult morphological defects of Egfrt1. Rarely survives as transheterozygote with the semi-viable Egfrtop-CA allele.

      (Clifford and Schupbach, 1994)

      Embryos shifted from the restrictive temperature to the permissive temperature after 5 hours of development display defective germband retraction and defective dorsal/ventral patterning. The cell fate of ventral cells is shifted to lateral cells.

      (Raz and Shilo, 1993)

      Partially suppresses the "Ellipse" phenotype at both restrictive and permissive temperatures.

      (Baker and Rubin, 1992)

      Homozygotes show a weak zygotic embryonic lethal phenotype at the permissive temperature (18oC). They show a moderate or severe embryonic lethal phenotype, with all denticles being reduced in size at the restrictive temperature (29oC).

      (Clifford and Schupbach, 1992)

      At the permissive temperature, homozygous Egfrf1 embryos do not hatch, but the cuticle and CNS is similar to wild-type. At the restrictive temperature the cuticle and CNS show a severe phenotype, comparable to null Egfr alleles: the germ band does not retract, and the longitudinal axon tracts of the CNS are disarrayed. Temperature shifts at different times show that the phenotype can be dissected into at least five temporally and spatially distinct components, which affect many processes, including germ band retraction, CNS development, differentiation of cuticle secreting epidermal cells, differentiation of head structures, and differentiation and survival of midline glial cells.

      (Raz and Shilo, 1992)

      Homozygotes and hemizygotes display a weak 'flb' phenotype. Embryos produced from heteroallelic combination with Egfrt1 have a severe ventralised phenotype, reduction in size of their dorsal appendage.

      (Clifford and Schupbach, 1989)

      Weak lethal phenotype: embryos lack some head and telson structures and have some development of the ventral cuticle.

      (Schejter and Shilo, 1989)

      weak allele

      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhancer of
      Statement
      Reference

      Egfr[+]/Egfrf1 is an enhancer of visible | heat sensitive phenotype of peb1

      (Wilk et al., 2004)

      Egfrf1 is an enhancer of visible phenotype of gro1

      (Price et al., 1997)
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      Egfrf1 has somatic muscle cell | embryonic stage phenotype, enhanceable by SIIN

      (Buff et al., 1998)

      Egfrf1, spi1 has somatic muscle cell | embryonic stage phenotype, enhanceable by S[+]/SIIN

      (Buff et al., 1998)

      Egfrf1, SIIN has somatic muscle cell | embryonic stage phenotype, enhanceable by spi1

      (Buff et al., 1998)
      Suppressed by
      Statement
      Reference

      Egfrf1 has ventral denticle belt phenotype, suppressible by aope2d

      (Rogge et al., 1995)
      Enhancer of
      Statement
      Reference

      Egfr[+]/Egfrf1 is an enhancer of eye | heat sensitive phenotype of peb1

      (Wilk et al., 2004)

      Egfrf1 is an enhancer of phenotype of cswlf

      (Firth et al., 2000)

      Egfrt1/Egfrf1 is an enhancer of wing vein phenotype of rhove-1

      (Guichard et al., 1999)

      Egfrf1 is an enhancer of eye phenotype of hidGMR.PG

      (Kurada and White, 1998)

      Egfrf1 is an enhancer of eye phenotype of gro1

      (Price et al., 1997)

      Egfrf1 is an enhancer of ommatidium phenotype of gro1

      (Price et al., 1997)

      Egfrf1 is an enhancer of interommatidial bristle phenotype of gro1

      (Price et al., 1997)

      Egfrf1 is an enhancer of prothoracic leg phenotype of gro1

      (Price et al., 1997)
      Suppressor of
      Statement
      Reference

      Egfrf1 is a suppressor of phenotype of rhohs.sev

      (Freeman, 1994)
      NOT Suppressor of
      Statement
      Reference

      Egfrf1 is a non-suppressor of denticle phenotype of wgl-17

      (Szuts et al., 1997)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      When Egfrf1/Egfrt1; rhove-1/rhove-1 flies are raised at the permissive temperature (18oC) a wing vein phenotype is seen that is similar to (but slightly more severe than) rhove-1/rhove-1 alone; wing veins L4 and L5 have prominent distal truncations, L2 and L3 remain largely intact. When raised at the non-permissive temperature (29oC) there is an enhancement of the wing vein phenotype with all wing veins showing major truncations. This phenotype is seen if heat shocking occurs from 0 to 24 hours APF (after puparium formation).

      (Guichard et al., 1999)

      Embryos raised at 18oC have a mild muscle phenotype, which is dominantly enhanced by SIIN. The mild Egfrf1 muscle phenotype is not affected by one copy of spi1 alone, but spi1 shows additional enhancement of the muscle phenotype in combination with SIIN.

      (Buff et al., 1998)

      Enhances the eye reduction phenotype caused by WGMR.PG.

      (Kurada and White, 1998)

      Egfrf1; gro1/gro1 flies display an enhanced gro phenotype: ectopic compound eyes, fused or bifurcated legs. These phenotypes are almost exclusively restricted to males. The ectopic eyes are organised into arrays of ommatidia. They are frequently adjacent to the endogenous eye but separated by a discrete border and exhibit defects in ommatidial packing and the distribution of interommatidial bristles.

      (Price et al., 1997)

      Does not alter the denticle phenotype of wgl-17 larvae.

      (Szuts et al., 1997)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      Selected as: Embryonic lethal.

      Comments
      Comments

      Weak allele.

      (Schejter and Shilo, 1989)

      Temperature shift analysis identifies two distinct times at which Egfr is required for embryonic development. Germline clone analysis indicates that there is very little, if any, requirement for Egfr in the germline.

      (Clifford and Schupbach, 1992)

      Class I allele.

      (Clifford and Schupbach, 1994)
      External Crossreferences and Linkouts ( 2 )
      Crossreferences
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      Synonyms and Secondary IDs (18)
      References (41)