FB2024_03 , released June 25, 2024
Allele: Dmel\haync2rv1
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General Information
Symbol
Dmel\haync2rv1
Species
D. melanogaster
Name
FlyBase ID
FBal0005385
Feature type
allele
Associated gene
Dmel\hay
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

antimorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

antimorphic allele - genetic evidence

(Regan and Fuller, 1990)
Mutagen
ethyl methanesulfonate
(Regan and Fuller, 1990)
Progenitor genotype
haync2
(Mounkes and Fuller, 1999, Regan and Fuller, 1990)
Cytology
Description

Mutation lies in helicase domain VI.

(Mounkes and Fuller, 1999)

Nucleotide substitution: C?T.

(Mounkes and Fuller, 1999)

Amino acid replacement: Q657term.

(Mounkes and Fuller, 1999)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3L:10,633,484..10,633,484 [+]
Nucleotide change:

C10633484T

Reported nucleotide change:

C?T

Amino acid change:

Q654term | hay-PA; Q654term | hay-PB

Reported amino acid change:

Q657term

Comment:

The amino acid numbering differs between FBrf0108244 and the reference sequence. The positions and nature of the base substitutions in the mutants are derived from the sequence in Fig. 1 and from Table 1, and provide unambiguous assignments.

(Mounkes and Fuller, 1999)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      haync2rv1/+ mutants do not show any changes in the number of cells in mitosis in the third instar larval wing discs compared to wild-type controls.

      (Lee et al., 2015)

      haync2/haync2rv1 animals show 47.9% viability. 19.9% of haync2/haync2rv1 adults have deformed and fragile macrochaetae and microchaetae, 21.5% have a reduction of the endocuticular layer of the abdominal cuticle and 14.3% show loss of wing marginal regions and blistered wings. One copy of hayTTD.Hsp83 or hayXPCS.Hsp83 decreases the viability of haync2/haync2rv1 flies dramatically.

      (Merino et al., 2002)

      Lethal over deficiency of hay. Heterozygotes exhibit defects in the structure and function of the male meiotic spindle.

      (Regan and Fuller, 1990)

      recessive larval lethal

      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Enhancer of
      Suppressor of
      Statement
      Reference

      hay[+]/haync2rv1 is a suppressor of decreased body size | third instar larval stage phenotype of MycP0

      (Lee et al., 2015)

      hay[+]/haync2rv1 is a suppressor of visible phenotype of Scer\GAL4Bx-MS1096, p53GUS.PB

      (Merino et al., 2002)
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      hay[+]/haync2rv1, hfpEP3058 has wing disc | third instar larval stage phenotype, suppressible by MycG0354

      (Lee et al., 2015)
      Enhancer of
      Statement
      Reference

      hay[+]/haync2rv1 is an enhancer of wing disc | third instar larval stage phenotype of hfpEP3058

      (Lee et al., 2015)
      Suppressor of
      Statement
      Reference

      hay[+]/haync2rv1 is a suppressor of wing phenotype of Scer\GAL4Bx-MS1096, p53GUS.PB

      (Merino et al., 2002)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The large body size as well as the elevated proliferation rate in the wing disc characteristic for the hfpEP3058/hfpEP3058 third instar larvae is further increased by combination with haync2rv1 in heterozygous state. The increased body size of hfpEP3058/hfpEP3058;haync2rv1/+ larvae can be partially suppressed by driving driving hfp expression from the UAS included in the hfpEP3058 P-element insertion allele under the control of the Scer\GAL4arm.PU driver.

      The severe body overgrowth seen in hfpEP3058/hfpEP3058;haync2rv1/+ third instar larvae can be rescued by combination with MycP0 in heterozygous state.

      The small body size characteristic for MycP0/+ third instar larvae can be suppressed by combination with haync2rv1 in heterozygous state.

      The severe body overgrowth seen in hfpEP3058/hfpEP3058;haync2rv1/+ third instar larvae can be rescued by combination with MycP0 in heterozygous state and the increased number of mitotic cells can be suppressed by combination with MycG0354/+.

      haync2rv1/+;MycG0354/+ double heterozygotes do not show significant changes in the number of mitotic cells in the third instar larval wing disc compared to controls.

      (Lee et al., 2015)

      The wing defects caused by expression of p53GUS.PB under the control of Scer\GAL4Bx-MS1096 are completely suppressed by haync2rv1/+.

      (Merino et al., 2002)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      HayRV1
      (Lee et al., 2015)
      Haync2rv1
      (Lee et al., 2015)
      haync2rv1
      Name Synonyms
      Secondary FlyBase IDs
        References (4)