FlyBase Allele Report: Dmel\Nup154[01501]

General Information

Symbol

Dmel\Nup154⁰¹⁵⁰¹

Species

D. melanogaster

Name

FlyBase ID

FBal0007979

Feature type

allele

Associated gene

Dmel\Nup154

Associated Insertion(s)

P{PZ}Nup154⁰¹⁵⁰¹

Carried in Construct

Also Known As

l(2)01501

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

Progenitor genotype

Associated Insertion(s)

P{PZ}Nup154⁰¹⁵⁰¹

Cytology

Description

P{PZ} insertion approximately 100bp upstream from the start of the predicted Nup154 open reading frame. The insertion is in the opposite 5' to 3' orientation to the direction of Nup154 transcription.

(Kiger et al., 1999)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:11,004,888..11,004,889 [+]

(McClure and Schubiger, 2008, Beaton, 2000.1.31, Kiger et al., 1999, Mesilaty-Gross et al., 1999, Spradling et al., 1999, Gigliotti et al., 1998, Jackle and Janning, 1998.8.10, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | third instar larval stage

(Kiger et al., 1999)

decreased body size | third instar larval stage

(Kiger et al., 1999)

decreased cell number | spermatogenesis (with Nup154^RV5)

(Kiger et al., 1999)

lethal | recessive

(Beaton, 2000.1.31, Kiger et al., 1999, Spradling et al., 1999, Gigliotti et al., 1998, Jackle and Janning, 1998.8.10)

Phenotype Manifest In

egg chamber

(Gigliotti et al., 1998)

embryonic/larval brain

(Kiger et al., 1999)

embryonic/larval lymph gland

(Kiger et al., 1999)

embryonic/larval optic lobe

(Kiger et al., 1999)

imaginal disc

(Kiger et al., 1999)

meiotic cell cycle | male

(Kiger et al., 1999)

ovariole

(Gigliotti et al., 1998)

spermatid

(Kiger et al., 1999)

testis

(Kiger et al., 1999)

Detailed Description

Statement

Reference

Nup154⁰¹⁵⁰¹/Nup154^RV5 males have a "meiotic arrest" phenotype in the testis; early spermatogenic stages appear normal and spermatocytes accumulate, but cells in meiotic divisions and spermatid differentiation are lacking. Larvae transheterozygous for Nup154⁰¹⁵⁰¹ and either Nup154² or Nup154^k08204 die throughout the early larval stages, with survivors found until only just after the third instar larval moult. Until the end of the second instar stage, the larvae appear morphologically similar to their heterozygous siblings in body and tissue size. After the third instar moult, the larvae fail to grow and show a "classic mitotic mutant" phenotype, including reduced overall body size and a reduction in the size of larval organs that normally contain proliferating cells. Homozygous, hemizygous or transheterozygous third instar larvae have smaller brains than their age-matched control siblings. Although the neuropil is relatively normal in size, the optic lobes are greatly reduced and imaginal discs are tiny and difficult if not impossible to locate. The lymph glands are also reduced in size in third instar larvae.

(Kiger et al., 1999)

Nup154^tlp2/Nup154⁰¹⁵⁰¹ ovarioles have a "string of pearls" morphology in which egg chambers have degenerated before entering vitellogenic stages.

(Gigliotti et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Nup154⁰¹⁵⁰¹/Nup154[+] is a suppressor of visible | somatic clone phenotype of wg^Act5C.PS

(McClure and Schubiger, 2008)

Phenotype Manifest In

Suppressor of

Statement

Reference

Nup154⁰¹⁵⁰¹/Nup154[+] is a suppressor of leg | somatic clone phenotype of wg^Act5C.PS

(McClure and Schubiger, 2008)

Nup154⁰¹⁵⁰¹/Nup154[+] is a suppressor of wing | ectopic | somatic clone phenotype of wg^Act5C.PS

(McClure and Schubiger, 2008)

Additional Comments

Genetic Interactions

Statement

Reference

The frequency of leg-to-wing transdetermination caused by expression of wg^Act5C.PS in clones during the mid-second larval instar is dominantly suppressed by Nup154⁰¹⁵⁰¹.

(McClure and Schubiger, 2008)

Nup154⁰¹⁵⁰¹ cup⁸ double heterozygous females show no obvious defects in egg chamber development.

(Grimaldi et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Nup154^tlp2

(Gigliotti et al., 1998)

Nup154^tlp1

(Gigliotti et al., 1998)