Egg chamber maturation proceeds normally in homozygous females.

Homozygous females are almost completely sterile and produce very few eggs compared to Nup154^tlp1 homozygous females. Nup154^tlp2/Nup154⁰¹⁵⁰¹ ovarioles have a "string of pearls" morphology in which egg chambers have degenerated before entering vitellogenic stages. Homozygous Nup154^tlp2 egg chambers reach later developmental stages but have abnormal degrees of nurse cell chromatin condensation compared to wild-type; in the most extreme cases nuclei retain the five blocks or polytene morphology characteristic of earlier developmental stages. Testes from homozygous pupae and adults are 2 to 3 times smaller than wild-type. Only primary spermatocytes and cyst cells are seen in most testes, and meiotic figures are never seen. Some cysts contain fewer than the normal number of 16 spermatocytes. As in wild-type, centrioles duplicate at the beginning of the spermatocyte growth phase and are associated with two small asters. At the end of prophase, centrioles elongate at least threefold and become associated with large asters, as in wild-type. Meiotic spindles do not form. Although meiotic divisions do not occur, other aspects of spermiogenesis continue; in older cysts the germ cell microtubule cytoskeleton appears to have undergone spatial reorganisation - microtubules are elongated and arranged in circular bundles that form a dense shell below the plasma membrane of round germ cells containing one or two chromatin masses. Small anucleate cytoplasmic fragments surrounded by microtubule shells are also seen. Irregular axonemes are sometimes present. The nuclear membrane of homozygous spermatocytes form small evaginations, probably due to the nuclear envelope bulging out near nuclear pores.

Nup154^tlp2, cup⁰¹³⁵⁵ has ovariole phenotype

Nup154^tlp2, cup⁰¹³⁵⁵ has chromosome & nurse cell phenotype