FlyBase Allele Report: Dmel\Pkn[06736]

(Rudrapatna et al., 2014, Sotillos and Campuzano, 2000, Lu and Settleman, 1999, Spradling et al., 1999, Zhang et al., 1996, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell shape | third instar larval stage (with Df(2R)w73-1)

(Tsankova et al., 2017)

lethal

(Betson and Settleman, 2007)

lethal | recessive

(Lu and Settleman, 1999, Spradling et al., 1999)

Phenotype Manifest In

Detailed Description

Statement

Reference

Whereas wild-type larval neuroblasts during asymmetric cell division expand the apical cortex during anaphase, the Pkn⁰⁶⁷³⁶/Df(2R)w73-1 mutant neuroblasts first expand the basal cortex which subsequently retracts and then the apical cortex is enlarged, causing a temporarily inverted asymmetric anaphase figure followed by a symmetric one and finally normal asymmetric figure. The cell curvature along the apical-basal neuroblast cortex from early anaphase to telophase is also abnormal in Pkn⁰⁶⁷³⁶/Df(2R)w73-1 neuroblasts: While wild-type cells show a cortical ingression (negative curvature) in a basally shifted position at early anaphase stage, this ingression develops into a pronounced cleavage furrow but remain at approximately the location. In contrast, the mutant neuroblasts initiate furrowing close to the apical cortex and this ingression then shifts basally during anaphase.

Even prior to anaphase, during metaphase, the Pkn⁰⁶⁷³⁶/Df(2R)w73-1 neuroblasts display significant cell shape deformation of the apical cortex - both positive and negative changes in curvature while in wild-type neuroblasts the curvature change very little during metaphase.

This abnormal shape dynamics is underpinned by spatiotemporal aberrations in myosin localization pattern and activity.

(Tsankova et al., 2017)

Egg length is significantly reduced in Pkn⁰⁶⁷³⁶/Pkn⁰⁶⁷³⁶ female germline clones.

During nurse cell dumping, egg chambers mutant for Pkn⁰⁶⁷³⁶/Pkn⁰⁶⁷³⁶ show increased nurse cell-to-oocyte membrane collapse, leading to abnormal presence of ring canals, border cells and/or nurse cell nuclei in the oocyte cytoplasm.

Females with Pkn⁰⁶⁷³⁶/Pkn⁰⁶⁷³⁶ germline clones show intermediate abnormal accumulation of F-actin in nurse cells of stage 10 egg chambers.

(Ferreira et al., 2014)

No adult homozygotes emerge. 10% of homozygous embryos show a dorsal closure phenotype. Germline clones produced mutant embryos, about 55% of which showed defects in dorsal closure. No defects in patterning or in the central and peripheral nervous systems or somatic muscles were found. When embryos from germline clone mothers are examined for cell shape, despite apparently normal leading edge cell stretching, all epidermal cells adopt an unstretched polygonal shape.

(Lu and Settleman, 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Pkn⁰⁶⁷³⁶ has lethal phenotype, suppressible by Pkc53E::Pkn^hs.PB

(Betson and Settleman, 2007)

NOT suppressed by

Statement

Reference

Pkn⁰⁶⁷³⁶ has lethal phenotype, non-suppressible by Pkn::Rok^hs.PB

(Betson and Settleman, 2007)

Pkn⁰⁶⁷³⁶ has lethal phenotype, non-suppressible by Pak::Pkn^hs.PB

(Betson and Settleman, 2007)

Pkn⁰⁶⁷³⁶ has lethal phenotype, non-suppressible by Pak::Pkc53E^hs.PB

(Betson and Settleman, 2007)

Pkn⁰⁶⁷³⁶ has lethal phenotype, non-suppressible by Pkc53E::Rok^hs.PB

(Betson and Settleman, 2007)

Pkn⁰⁶⁷³⁶ has lethal phenotype, non-suppressible by Pkn::Mmus\Rtkn^hs.PB

(Betson and Settleman, 2007)

Pkn⁰⁶⁷³⁶ has lethal phenotype, non-suppressible by Pkc53E::Mmus\Rtkn^hs.PB

(Betson and Settleman, 2007)

Suppressor of

Statement

Reference

Pkn⁰⁶⁷³⁶/Pkn[+] is a suppressor of visible phenotype of LIMK1^UAS.cCa, Scer\GAL4^en-e16E

(Chen et al., 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

Pkn⁰⁶⁷³⁶ has embryo | dorsal closure stage phenotype, enhanceable by Rho1^72R

(Lu and Settleman, 1999)

Pkn⁰⁶⁷³⁶ has embryonic epidermis | dorsal phenotype, enhanceable by Rho1^72R

(Lu and Settleman, 1999)

Pkn⁰⁶⁷³⁶ has embryo | dorsal closure stage phenotype, enhanceable by bsk¹

(Lu and Settleman, 1999)

Pkn⁰⁶⁷³⁶ has embryonic epidermis | dorsal phenotype, enhanceable by bsk¹

(Lu and Settleman, 1999)

Suppressor of

Statement

Reference

Pkn⁰⁶⁷³⁶/Pkn[+] is a suppressor of wing phenotype of LIMK1^UAS.cCa, Scer\GAL4^en-e16E

(Chen et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Pkc53E::Pkn^hs.PB (using a reduced heat shock regime of 15 minutes per day) can partially rescue the lethality of Pkn⁰⁶⁷³⁶.

(Betson and Settleman, 2007)

Pkn⁰⁶⁷³⁶/+ suppresses the mutant wing phenotype caused by expression of LIMK1^Scer\UAS.cCa under the control of Scer\GAL4^en-e16E (the % of wings with normal morphology at 18^oC is increased from 9% to 27%).

(Chen et al., 2004)

Pkn³ shows no interaction with Sb⁷⁰ or Sb^63b (assayed in terms of a malformed leg phenotype). Pkn⁰⁶⁷³⁶ shows a weak dominant enhancement of leg malformation in Sb^sbd-201/Sb^sbd-1 flies.

(Bayer et al., 2003)

When Pkn⁰⁶⁷³⁶ germline clone mothers are crossed with bsk¹ or Rho1^72R fathers, 76% or 68% of the embryos showed an identical dorsal closure phenotype, respectively, an enhancement of the dorsal closure phenotype caused by Pkn⁰⁶⁷³⁶ germline clones alone.

(Lu and Settleman, 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Pkn⁰⁶⁷³⁶ is partially rescued by Pkn^hs.PL

(Betson and Settleman, 2007)

Pkn⁰⁶⁷³⁶ is partially rescued by Pkn^G58A.hs

(Betson and Settleman, 2007)

Pkn⁰⁶⁷³⁶ is partially rescued by Pkn^ΔACC1.hs

(Betson and Settleman, 2007)