FlyBase Allele Report: Dmel\LIMK1[UAS.cCa]

General Information

Symbol

Dmel\LIMK1^UAS.cCa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Chen

FlyBase ID

FBal0156297

Feature type

allele

Associated gene

Dmel\LIMK1

Associated Insertion(s)

Carried in Construct

P{UAS-LIMK1.C}

Also Known As

UAS-Dlimk

Key Links

Transgenic product class

wild_type

(Chen et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Chen et al., 2004)

Mutagen

in vitro construct

(Chen et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-LIMK1.C}

Cytology

Description

UAS regulatory sequences drive expression of LIMK1.

(Chen et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

LIMK1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^en-e16E

(Chen et al., 2004)

Phenotype Manifest In

dorsal mesothoracic disc & actin filament, with Scer\GAL4^en-e16E

(Chen et al., 2004)

wing, with Scer\GAL4^en-e16E

(Chen et al., 2005, Chen et al., 2004)

wing vein, with Scer\GAL4^en-e16E

(Chen et al., 2004)

Detailed Description

Statement

Reference

Expression of LIMK1^Scer\UAS.cCa in the embryonic nervous system under the control of Scer\GAL4^elav.PU does not affect ventral nerve cord morphology.

(Verdier et al., 2006)

Expression of LIMK1^Scer\UAS.cCa in the posterior region of the wing under the control of Scer\GAL4^en-e16E results in multiple defects in this region.

(Chen et al., 2005)

Expression of LIMK1^Scer\UAS.cCa under the control of Scer\GAL4^en-e16E results in loss of wing tissue, wing blistering and increased width and fusion of veins in the posterior wing compartment. Wing discs expressing LIMK1^Scer\UAS.cCa under the control of Scer\GAL4^en-e16E show a prominent accumulation of F-actin and aberrant actin organisation. Flies co-expressing LIMK1^Scer\UAS.cCa and LIMK1^{D522A.Scer\UAS} under the control of Scer\GAL4^en-e16E have normal wings.

(Chen et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

LIMK1^UAS.cCa, Scer\GAL4^en-e16E has visible phenotype, suppressible by Pkn⁰⁶⁷³⁶/Pkn[+]

(Chen et al., 2004)

LIMK1^UAS.cCa, Scer\GAL4^en-e16E has visible phenotype, suppressible by Rho1[+]/Rho1^k02107b

(Chen et al., 2004)

LIMK1^UAS.cCa, Scer\GAL4^en-e16E has visible phenotype, suppressible by RhoGEF2⁰⁴²⁹¹/RhoGEF2[+]

(Chen et al., 2004)

LIMK1^UAS.cCa, Scer\GAL4^en-e16E has visible phenotype, suppressible by bs[+]/bs²

(Chen et al., 2004)

LIMK1^UAS.cCa, Scer\GAL4^en-e16E has visible phenotype, suppressible by dia[+]/dia¹

(Chen et al., 2004)

LIMK1^UAS.cCa, Scer\GAL4^en-e16E has visible phenotype, suppressible by dia[+]/dia^k07135

(Chen et al., 2004)

LIMK1^UAS.cCa, Scer\GAL4^en-e16E has visible phenotype, suppressible by rok[+]/Rok¹

(Chen et al., 2004)

LIMK1^UAS.cCa, Scer\GAL4^en-e16E has visible phenotype, suppressible by Rho1^72O/Rho1[+]

(Chen et al., 2004)

LIMK1^UAS.cCa, Scer\GAL4^en-e16E has visible phenotype, suppressible by RhoGAPp190^UAS.Tag:MYC, Scer\GAL4^en-e16E

(Chen et al., 2004)

NOT suppressed by

Statement

Reference

LIMK1^UAS.cCa, Scer\GAL4^en-e16E has visible phenotype, non-suppressible by Cdc42³

(Chen et al., 2004)

LIMK1^UAS.cCa, Scer\GAL4^en-e16E has visible phenotype, non-suppressible by Cdc42⁴

(Chen et al., 2004)

LIMK1^UAS.cCa, Scer\GAL4^en-e16E has visible phenotype, non-suppressible by Rac2³¹⁴

(Chen et al., 2004)

LIMK1^UAS.cCa, Scer\GAL4^en-e16E has visible phenotype, non-suppressible by mbc^2.35

(Chen et al., 2004)

LIMK1^UAS.cCa, Scer\GAL4^en-e16E has visible phenotype, non-suppressible by Pak^KR.UAS, Scer\GAL4^en-e16E

(Chen et al., 2004)

Phenotype Manifest In

Suppressed by

Statement

Reference

LIMK1^UAS.cCa, Scer\GAL4^en-e16E has wing phenotype, suppressible by Pkn⁰⁶⁷³⁶/Pkn[+]

(Chen et al., 2004)

LIMK1^UAS.cCa, Scer\GAL4^en-e16E has wing phenotype, suppressible by Rho1^72O/Rho1[+]

(Chen et al., 2004)

LIMK1^UAS.cCa, Scer\GAL4^en-e16E has wing phenotype, suppressible by Rho1[+]/Rho1^k02107b

(Chen et al., 2004)

LIMK1^UAS.cCa, Scer\GAL4^en-e16E has wing phenotype, suppressible by RhoGEF2⁰⁴²⁹¹/RhoGEF2[+]

(Chen et al., 2004)

LIMK1^UAS.cCa, Scer\GAL4^en-e16E has wing phenotype, suppressible by bs[+]/bs²

(Chen et al., 2004)

LIMK1^UAS.cCa, Scer\GAL4^en-e16E has wing phenotype, suppressible by dia[+]/dia¹

(Chen et al., 2004)

LIMK1^UAS.cCa, Scer\GAL4^en-e16E has wing phenotype, suppressible by dia[+]/dia^k07135

(Chen et al., 2004)

LIMK1^UAS.cCa, Scer\GAL4^en-e16E has wing phenotype, suppressible by rok[+]/Rok¹

(Chen et al., 2004)

LIMK1^UAS.cCa, Scer\GAL4^en-e16E has wing phenotype, suppressible by RhoGAPp190^UAS.Tag:MYC, Scer\GAL4^en-e16E

(Chen et al., 2004)

NOT suppressed by

Statement

Reference

LIMK1^UAS.cCa, Scer\GAL4^en-e16E has wing phenotype, non-suppressible by Cdc42³

(Chen et al., 2004)

LIMK1^UAS.cCa, Scer\GAL4^en-e16E has wing phenotype, non-suppressible by Cdc42⁴

(Chen et al., 2004)

LIMK1^UAS.cCa, Scer\GAL4^en-e16E has wing phenotype, non-suppressible by Rac2³¹⁴

(Chen et al., 2004)

LIMK1^UAS.cCa, Scer\GAL4^en-e16E has wing phenotype, non-suppressible by mbc^2.35

(Chen et al., 2004)

LIMK1^UAS.cCa, Scer\GAL4^en-e16E has wing phenotype, non-suppressible by Pak^KR.UAS, Scer\GAL4^en-e16E

(Chen et al., 2004)

Suppressor of

Statement

Reference

LIMK1^UAS.cCa, Scer\GAL4^32B is a suppressor of wing phenotype of Pax^UAS.cCa, Scer\GAL4^32B

(Chen et al., 2005)

LIMK1^UAS.cCa, Scer\GAL4^en-e16E is a suppressor of wing phenotype of Pax^UAS.cCa, Scer\GAL4^en-e16E

(Chen et al., 2005)

Other

Statement

Reference

LIMK1^UAS.cCa, Rok^cat.UAS.cVa, Scer\GAL4^en-e16E has larval ventral nerve cord | embryonic stage phenotype

(Verdier et al., 2006)

LIMK1^UAS.cCa, RhoGAPp190^UAS.Tag:MYC, Scer\GAL4^en-e16E has crossvein | ectopic phenotype

(Chen et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of LIMK1^Scer\UAS.cCa and rok^cat.Scer\UAS in the embryonic nervous system under the control of Scer\GAL4^elav.PU results in breaks along the ventral nerve cord.

(Verdier et al., 2006)

Expression of LIMK1^Scer\UAS.cCa suppresses the wing phenotype observed in flies expressing Pax^Scer\UAS.cCa under the control of Scer\GAL4^32B.

The blistered wing phenotype observed in flies expressing Pax^Scer\UAS.cCa in the posterior region of the wing under the control of Scer\GAL4^en-e16E is suppressed by co-expression of LIMK1^Scer\UAS.cCa.

(Chen et al., 2005)

The mutant wing phenotype caused by expression of LIMK1^Scer\UAS.cCa under the control of Scer\GAL4^en-e16E is suppressed if the flies are also heterozygous for one of the following alleles (% of wings with normal morphology at 18^oC in combination with each allele is given in brackets, value is 9% for control flies expressing LIMK1^Scer\UAS.cCa under the control of Scer\GAL4^en-e16E in an otherwise wild-type background); Rho1^72O (97%), Rho1^k02107b (95%), RhoGEF2⁰⁴²⁹¹ (87%), dia¹ (98%), dia^k07135 (97%), rok¹ (64%), bs² (81%), Pkn⁰⁶⁷³⁶ (27%). The mutant wing phenotype caused by expression of LIMK1^Scer\UAS.cCa under the control of Scer\GAL4^en-e16E is largely unaffected if the flies are also heterozygous for one of the following alleles (% of wings with normal morphology at 18^oC in combination with each allele is given in brackets, value is 9% for control flies expressing LIMK1^Scer\UAS.cCa under the control of Scer\GAL4^en-e16E in an otherwise wild-type background); Rac1^J11 (23%), Rac2³¹⁴ (12%), Cdc42³ (10%), Cdc42⁴ (10%), mbc^2.35 (9%). Co-expression of Pak^KR.Scer\UAS has very little effect on the mutant wing phenotype caused by expression of LIMK1^Scer\UAS.cCa under the control of Scer\GAL4^en-e16E (the % of wings with normal morphology at 18^oC is 16% compared to 9% for control flies expressing LIMK1^Scer\UAS.cCa under the control of Scer\GAL4^en-e16E in an otherwise wild-type background). Co-expression of RhoGAPp190^{Scer\UAS.T:Hsap\MYC} suppresses the mutant wing phenotype caused by expression of LIMK1^Scer\UAS.cCa under the control of Scer\GAL4^en-e16E (the % of wings with normal morphology at 18^oC is increased from 9% to 85%). Approximately 63% of the flies show a mild extra crossvein phenotype.

(Chen et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

LIMK1^Scer\UAS.cCa

LIMK1^UAS.cCa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Chen

(Chen et al., 2004)

Secondary FlyBase IDs

References (3)

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