FlyBase Allele Report: Dmel\Pax[UAS.cCa]

General Information

Symbol

Dmel\Pax^UAS.cCa

Species

D. melanogaster

Name

FlyBase ID

FBal0246047

Feature type

allele

Associated gene

Dmel\Pax

Associated Insertion(s)

Carried in Construct

P{UAS-Pax.C}

Also Known As

UAS-Dpax

Key Links

Transgenic product class

wild_type

(Chen et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Chen et al., 2005)

Mutagen

in vitro construct

(Chen et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-Pax.C}

Cytology

Description

UASt regulatory sequences drive expression of Pax.

(Chen et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Pax

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | larval stage | heat sensitive, with Scer\GAL4^hs.PB

(Chen et al., 2005)

lethal | pupal stage | heat sensitive, with Scer\GAL4^hs.PB

(Chen et al., 2005)

partially lethal - majority live, with Scer\GAL4^en-e16E

(Chen et al., 2008)

partially lethal - majority live, with Scer\GAL4^ptc-559.1

(Chen et al., 2008)

visible, with Scer\GAL4^32B

(Chen et al., 2008)

visible | heat sensitive, with Scer\GAL4^hs.PB

(Chen et al., 2005)

Phenotype Manifest In

dorsal transverse muscle cell, with Scer\GAL4^eve.eme

(Bataillé et al., 2010)

embryonic/larval epidermis | heat sensitive, with Scer\GAL4^hs.PB

(Chen et al., 2005)

leg | heat sensitive, with Scer\GAL4^hs.PB

(Chen et al., 2005)

muscle cell of dorsal acute muscle 1, with Scer\GAL4^eve.eme

(Bataillé et al., 2010)

muscle cell of ventral transverse muscle 1, with Scer\GAL4^eve.eme

(Bataillé et al., 2010)

nucleus | increased number, with Scer\GAL4^eve.eme

(Bataillé et al., 2010)

wing, with Scer\GAL4^32B

(Chen et al., 2008, Chen et al., 2005)

wing, with Scer\GAL4^en-e16E

(Chen et al., 2005)

wing | heat sensitive, with Scer\GAL4^hs.PB

(Chen et al., 2005)

wing vein | ectopic | heat sensitive, with Scer\GAL4^hs.PB

(Chen et al., 2005)

Detailed Description

Statement

Reference

Overexpression of Pax^Scer\UAS.cCa under the control of Scer\GAL4^eve.eme increases the number of nuclei in DA1, DT1 and VT1 muscles, whereas VA2 is not affected.

Overexpression of Pax^Scer\UAS.cCa under the control of Scer\GAL4^eve.eme results in accelerated embryonic myoblast fusion in DA1 compared with wild-type.

(Bataillé et al., 2010)

Overexpression of Pax^Scer\UAS.cCa via Scer\GAL4^32B in the developing wing results in crumpled and blistered wings.

Flies expressing Pax^Scer\UAS.cCa under the control of either Scer\GAL4^ptc-559.1 or Scer\GAL4^en-e16E are largely viable.

(Chen et al., 2008)

Expression of Pax^Scer\UAS.cCa in first instar larvae under the control of Scer\GAL4^hs.PB results in lethality. Larvae die within 48 hours after heat shock, and exhibit an aberrant dentical epidermis. Overexpression of Pax^Scer\UAS.cCa at the late larval-pupal stages results in a pupal lethal phenotype with a few escapers. These flies survive to adulthood and exhibit extra wing veins, blistered wing, and malformed leg phenotypes.

Expression of Pax^Scer\UAS.cCa in the wing using Scer\GAL4^32B results in a highly penetrant poorly expanded and blistered wing phenotype.

Expression of Pax^Scer\UAS.cCa in the posterior region of the wing under the control of Scer\GAL4^en-e16E results in a blistered-wing defect in this region.

(Chen et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Pax^UAS.cCa, Scer\GAL4^32B has visible phenotype, suppressible by Atg1^EP3193/Atg1[+]

(Chen et al., 2008)

Pax^UAS.cCa, Scer\GAL4^32B has visible phenotype, suppressible by Atg1^RNAi.UAS, Scer\GAL4^32B

(Chen et al., 2008)

Pax^UAS.cCa, Scer\GAL4^32B has visible phenotype, suppressible by EcR^W53st/EcR[+]

(Chen et al., 2008)

Pax^UAS.cCa, Scer\GAL4^32B has visible phenotype, suppressible by EcR[+]/EcR²²⁵

(Chen et al., 2008)

NOT suppressed by

Statement

Reference

Pax^UAS.cCa, Scer\GAL4^32B has visible phenotype, non-suppressible by Atg1[+]/Atg1^Δ3D

(Chen et al., 2008)

Enhancer of

Statement

Reference

Pax^UAS.cCa, Scer\GAL4^ptc-559.1 is an enhancer of partially lethal - majority live phenotype of Atg1^UAS.cCa, Scer\GAL4^ptc-559.1

(Chen et al., 2008)

Pax^UAS.cCa, Scer\GAL4^en-e16E is an enhancer of partially lethal - majority live phenotype of Atg1^UAS.cCa, Scer\GAL4^en-e16E

(Chen et al., 2008)

Phenotype Manifest In

Suppressed by

Statement

Reference

Pax^UAS.cCa, Scer\GAL4^32B has wing phenotype, suppressible by EcR^W53st/EcR[+]

(Chen et al., 2008)

Pax^UAS.cCa, Scer\GAL4^32B has wing phenotype, suppressible by EcR[+]/EcR²²⁵

(Chen et al., 2008)

Pax^UAS.cCa, Scer\GAL4^32B has wing phenotype, suppressible by Atg1^EP3193/Atg1[+]

(Chen et al., 2008)

Pax^UAS.cCa, Scer\GAL4^32B has wing phenotype, suppressible by Atg1^RNAi.UAS, Scer\GAL4^32B

(Chen et al., 2008)

Pax^UAS.cCa, Scer\GAL4^32B has wing phenotype, suppressible by LIMK1^UAS.cCa, Scer\GAL4^32B

(Chen et al., 2005)

Pax^UAS.cCa, Scer\GAL4^32B has wing phenotype, suppressible by cdi^EP3319, Scer\GAL4^32B

(Chen et al., 2005)

Pax^UAS.cCa, Scer\GAL4^32B has wing phenotype, suppressible by bs^UAS.cHa, Scer\GAL4^32B

(Chen et al., 2005)

Pax^UAS.cCa, Scer\GAL4^32B has wing phenotype, suppressible by tsr[+]/tsr^k05633

(Chen et al., 2005)

Pax^UAS.cCa, Scer\GAL4^32B has wing phenotype, suppressible by ssh[+]/ssh⁰¹²⁰⁷

(Chen et al., 2005)

Pax^UAS.cCa, Scer\GAL4^en-e16E has wing phenotype, suppressible by LIMK1^UAS.cCa, Scer\GAL4^en-e16E

(Chen et al., 2005)

NOT suppressed by

Statement

Reference

Pax^UAS.cCa, Scer\GAL4^32B has wing phenotype, non-suppressible by Atg1[+]/Atg1^Δ3D

(Chen et al., 2008)

Enhancer of

Statement

Reference

Pax^UAS.cCa, Scer\GAL4^eve.eme is an enhancer of muscle cell of dorsal acute muscle 1 phenotype of Mp20^UAS.cBa, Scer\GAL4^eve.eme

(Bataillé et al., 2010)

Pax^UAS.cCa, Scer\GAL4^eve.eme is an enhancer of nucleus | increased number phenotype of Mp20^UAS.cBa, Scer\GAL4^eve.eme

(Bataillé et al., 2010)

Suppressor of

Statement

Reference

Pax^UAS.cCa, Scer\GAL4^eve.eme is a suppressor of muscle cell of dorsal acute muscle 1 phenotype of Scer\GAL4^eve.eme, mspo^UAS.cUa

(Bataillé et al., 2010)

Pax^UAS.cCa, Scer\GAL4^eve.eme is a suppressor of nucleus phenotype of Scer\GAL4^eve.eme, mspo^UAS.cUa

(Bataillé et al., 2010)

Other

Statement

Reference

Atg1^UAS.cCa, Pax^UAS.cCa, Scer\GAL4^ptc-559.1 has arista phenotype

(Chen et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Mp20^Scer\UAS.cBa and Pax^Scer\UAS.cCa under the control of Scer\GAL4^eve.eme induces a strong increase in the number of muscle nuclei that is much higher than the increase resulting from the overexpression of either transgene.

Co-expression of Pax^Scer\UAS.cCa and mspo^Scer\UAS.cUa under the control of Scer\GAL4^eve.eme restores the wild-type number of DA1 nuclei.

(Bataillé et al., 2010)

One copy of Atg1^EP3193 strongly suppresses the Scer\GAL4^32B-driven Pax^Scer\UAS.cCa-induced wing blistering.

One copy of Atg1^Δ3D fails to suppress the Scer\GAL4^32B-driven Pax^Scer\UAS.cCa-induced wing blistering.

Co-expression of Atg1^{dsRNA.Scer\UAS} significantly suppresses the Scer\GAL4^32B-driven Pax^Scer\UAS.cCa-induced wing defects.

One copy of EcR^W53st significantly suppresses the Scer\GAL4^32B-driven Pax^Scer\UAS.cCa-induced wing blistering.

One copy of EcR²²⁵ significantly suppresses the Scer\GAL4^32B-driven Pax^Scer\UAS.cCa-induced wing blistering.

Flies overexpressing both Pax^Scer\UAS.cCa and Atg1^Scer\UAS.cCa under the control of Scer\GAL4^ptc-559.1 show increased lethality, compared to lines overexpressing either transgene alone. Over 90% of the flies expressing both Pax^Scer\UAS.cCa and Atg1^Scer\UAS.cCa die at pupal stages.

Flies overexpressing both Pax^Scer\UAS.cCa and Atg1^Scer\UAS.cCa under the control of Scer\GAL4^en-e16E show increased lethality, compared to lines overexpressing either transgene alone.

Aristae of flies overexpressing both Pax^Scer\UAS.cCa and Atg1^Scer\UAS.cCa driven by Scer\GAL4^ptc-559.1 are often missing or greatly shortened.

(Chen et al., 2008)

Expression of LIMK1^Scer\UAS.cCa, cdi^EP3319 or bs^Scer\UAS.cHa in flies expressing Pax^Scer\UAS.cCa under the control of Scer\GAL4^32B suppresses the blistered-wing phenotype.

The blistered wing phenotype observed in flies expressing Pax^Scer\UAS.cCa under the control of Scer\GAL4^32B is dominantly suppressed in a tsr^k05633/+ or ssh⁰¹²⁰⁷/+ background.

The blistered wing phenotype observed in flies expressing Pax^Scer\UAS.cCa in the posterior region of the wing under the control of Scer\GAL4^en-e16E is suppressed by co-expression of LIMK1^Scer\UAS.cCa.

(Chen et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Pax^UAS.cCa is rescued by Scer\GAL4^32B/Pax^RNAi.hs

(Chen et al., 2005)

Comments

The blistered-wing phenotype observed in flies expressing Pax^Scer\UAS.cCa under the control of Scer\GAL4^32B is suppressed by co-expression of Pax^dsRNA.hs.

(Chen et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Pax^Scer\UAS.cCa

Pax^UAS.cCa

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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