FlyBase Allele Report: Dmel\Atg1[UAS.cCa]

General Information

Symbol

Dmel\Atg1^UAS.cCa

Species

D. melanogaster

Name

FlyBase ID

FBal0267076

Feature type

allele

Associated gene

Dmel\Atg1

Associated Insertion(s)

Carried in Construct

P{UAS-Atg1.C}

Also Known As

UAS-Atg1

Key Links

Transgenic product class

wild_type

(Chen et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Chen et al., 2008)

Mutagen

in vitro construct

(Chen et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-Atg1.C}

Cytology

Description

UAS regulatory sequences drive expression of Atg1.

(Chen et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Atg1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates amyotrophic lateral sclerosis

modeled by Vap33^P58S.cRa.UAS

(Deivasigamani et al., 2014)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^elav-C155

(Deivasigamani et al., 2014)

increased cell death, with Scer\GAL4^ptc-559.1

(Chen et al., 2008)

partially lethal - majority live, with Scer\GAL4^en-e16E

(Chen et al., 2008)

partially lethal - majority live, with Scer\GAL4^ptc-559.1

(Chen et al., 2008)

visible, with Scer\GAL4^ptc-559.1

(Tang et al., 2011, Chen et al., 2008)

Phenotype Manifest In

anterior crossvein, with Scer\GAL4^ptc-559.1

(Tang et al., 2011, Chen et al., 2008)

autophagosome | increased number, with Scer\GAL4^ptc-559.1

(Chen et al., 2008)

filamentous actin, with Scer\GAL4^ptc-559.1

(Tang et al., 2011)

intestinal stem cell | chemical conditional, with Scer\GAL4^NP5130

(Tang et al., 2013)

macrochaeta, with Scer\GAL4^ptc-559.1

(Chen et al., 2008)

terminal bouton, with Scer\GAL4^elav-C155

(Deivasigamani et al., 2014)

wing disc | third instar larval stage, with Scer\GAL4^ptc-559.1

(Tang et al., 2011, Chen et al., 2008)

wing hair, with Scer\GAL4^ptc-559.1

(Chen et al., 2008)

Detailed Description

Statement

Reference

Expression of Atg1^Scer\UAS.cCa under the control of Scer\GAL4^elav-C155 reduces bouton size compared to controls.

(Deivasigamani et al., 2014)

Expression of Atg1^Scer\UAS.cCa under the control of either Scer\GAL4^esg-NP5130 results in impaired intestinal stem cell proliferation in response to paraquat.

(Tang et al., 2013)

Overexpression of Atg1^Scer\UAS.cCa in third instar wing imaginal discs under the control of Scer\GAL4^ptc-559.1 leads to abnormal cell morphology and F-actin reorganisation.

Overexpression of Atg1^Scer\UAS.cCa under the control of Scer\GAL4^ptc-559.1 results in the disappearance of anterior crossveins in adult wings.

(Tang et al., 2011)

Overexpression of Atg1^Scer\UAS.cCa in developing wing imaginal discs under the control of Scer\GAL4^ptc-559.1 induces a marked increase in autophagosome formation and increased cell death compared to controls.

Overexpression of Atg1^Scer\UAS.cCa by the Scer\GAL4^ptc-559.1 driver results in missing anterior crossveins, wing hair polarity defects and the loss of macrochaetae.

Flies expressing both Atg1^Scer\UAS.cCa and Atg1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ptc-559.1 exhibit normal wings.

Flies expressing Atg1^Scer\UAS.cCa under the control of either Scer\GAL4^ptc-559.1 or Scer\GAL4^en-e16E are largely viable.

(Chen et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Atg1^UAS.cCa, Scer\GAL4^ptc-559.1 has partially lethal - majority live phenotype, enhanceable by Pax^UAS.cCa, Scer\GAL4^ptc-559.1

(Chen et al., 2008)

Atg1^UAS.cCa, Scer\GAL4^en-e16E has partially lethal - majority live phenotype, enhanceable by Pax^UAS.cCa, Scer\GAL4^en-e16E

(Chen et al., 2008)

Suppressed by

Statement

Reference

Atg1^UAS.cCa, Scer\GAL4^ptc-559.1 has visible phenotype, suppressible by sqa^RNAi.UAS, Scer\GAL4^ptc-559.1

(Tang et al., 2011)

Atg1^UAS.cCa, Scer\GAL4^ptc-559.1 has visible phenotype, suppressible by EcR[+]/EcR²²⁵

(Chen et al., 2008)

Atg1^UAS.cCa, Scer\GAL4^ptc-559.1 has visible phenotype, suppressible by bs^UAS.cHa, Scer\GAL4^ptc-559.1

(Chen et al., 2008)

Suppressor of

Statement

Reference

Atg1^UAS.cCa, Scer\GAL4^elav-C155 is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^elav-C155, Vap33^P58S.cRa.UAS

(Deivasigamani et al., 2014)

Atg1^UAS.cCa, Scer\GAL4^elav-C155 is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^elav-C155, Vap33^UAS.cRa

(Deivasigamani et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

Atg1^UAS.cCa, Scer\GAL4^ptc-559.1 has anterior crossvein phenotype, suppressible by sqa^RNAi.UAS, Scer\GAL4^ptc-559.1

(Tang et al., 2011)

Atg1^UAS.cCa, Scer\GAL4^ptc-559.1 has anterior crossvein phenotype, suppressible by EcR[+]/EcR²²⁵

(Chen et al., 2008)

Atg1^UAS.cCa, Scer\GAL4^ptc-559.1 has wing hair phenotype, suppressible by EcR[+]/EcR²²⁵

(Chen et al., 2008)

Atg1^UAS.cCa, Scer\GAL4^ptc-559.1 has anterior crossvein phenotype, suppressible by bs^UAS.cHa, Scer\GAL4^ptc-559.1

(Chen et al., 2008)

Atg1^UAS.cCa, Scer\GAL4^ptc-559.1 has wing hair phenotype, suppressible by bs^UAS.cHa, Scer\GAL4^ptc-559.1

(Chen et al., 2008)

Suppressor of

Statement

Reference

Atg1^UAS.cCa, Scer\GAL4^elav-C155 is a suppressor of terminal bouton phenotype of Scer\GAL4^elav-C155, Vap33^P58S.cRa.UAS

(Deivasigamani et al., 2014)

Atg1^UAS.cCa, Scer\GAL4^elav-C155 is a suppressor of terminal bouton phenotype of Scer\GAL4^elav-C155, Vap33^UAS.cRa

(Deivasigamani et al., 2014)

Other

Statement

Reference

Atg1^UAS.cCa, Pax^UAS.cCa, Scer\GAL4^ptc-559.1 has arista phenotype

(Chen et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Atg1^Scer\UAS.cCa suppresses the enlarged bouton phenotype seen when Vap-33A^{P58S.cRa.Scer\UAS} is expressed under the control of Scer\GAL4^elav-C155.

Expression of Atg1^Scer\UAS.cCa suppresses the small bouton phenotype seen when Vap-33A^Scer\UAS.cRa is expressed under the control of Scer\GAL4^elav-C155.

(Deivasigamani et al., 2014)

Co-expression of sqa^{dsRNA.Scer\UAS} with Atg1^Scer\UAS.cCa under the control of Scer\GAL4^ptc-559.1 suppresses the anterior crossvein phenotype resulting from the overexpression of Atg1^Scer\UAS.cCa alone.

(Tang et al., 2011)

Flies overexpressing both Pax^Scer\UAS.cCa and Atg1^Scer\UAS.cCa under the control of Scer\GAL4^ptc-559.1 show increased lethality, compared to lines overexpressing either transgene alone. Over 90% of the flies expressing both Pax^Scer\UAS.cCa and Atg1^Scer\UAS.cCa die at pupal stages.

Flies overexpressing both Pax^Scer\UAS.cCa and Atg1^Scer\UAS.cCa under the control of Scer\GAL4^en-e16E show increased lethality, compared to lines overexpressing either transgene alone.

Aristae of flies overexpressing both Pax^Scer\UAS.cCa and Atg1^Scer\UAS.cCa driven by Scer\GAL4^ptc-559.1 are often missing or greatly shortened.

One copy of EcR²²⁵ suppresses the Scer\GAL4^ptc-559.1-driven Atg1^Scer\UAS.cCa-induced wing defects.

Overexpression of bs^Scer\UAS.cHa significantly suppresses the Scer\GAL4^ptc-559.1-driven Atg1^Scer\UAS.cCa-induced wing defects.

(Chen et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Atg1^Scer\UAS.cCa

Atg1^UAS.cCa

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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