FB2024_03 , released June 25, 2024
Allele: Dmel\dia1
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General Information
Symbol
Dmel\dia1
Species
D. melanogaster
Name
FlyBase ID
FBal0028144
Feature type
allele
Associated gene
Dmel\dia
Associated Insertion(s)
P{PZ}dia1
Carried in Construct
Also Known As
ms(2)04138
Key Links
Genomic Maps

Allele class

hypomorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

hypomorphic allele - genetic evidence

(Castrillon and Wasserman, 1994)
Mutagen
P-element activity
(Butler et al., 2001, Spradling et al., 1999, BDGP Project Members, 1994-1999, Castrillon and Wasserman, 1994, Castrillon et al., 1993)
Progenitor genotype
Associated Insertion(s)
P{PZ}dia1
Cytology
Description

P element inserted towards the 5' end of the dia gene transcription unit.

(Castrillon and Wasserman, 1994)
Allele components
Component
Use(s)
Inserted element
P{PZ}
Encoded product / tool
lacZ
Tagged with
Tag:NLS(P)
Mutations Mapped to the Genome
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Amnioserosa cells of dia1 mutant embryos are bigger, show larger perimeter, and present a more regular, more round shape, and exhibit a decrease in the amplitude of the cell contraction.

      dia1 embryos exhibit delayed dorsal closure phenotypes, such as decreased zipping speed, puckering of the dorsal epidermis, an unstretched leading edge, a malformed canthus and disintegration of the Amnioserosa.

      (Tóth et al., 2022)

      The actin ring is usually missing in the first meiotic division in homozygous males.

      (Giansanti et al., 1998)

      Homozygous males exhibit altered cyst cell behaviour. Male germ cells are rare and these germ cells fail to proliferate properly.

      (Gonczy and DiNardo, 1996)

      Spermatocytes and spermatids degenerate. By 5 days post-eclosion most mutant testes are devoid of germinal contents. Testes of newly eclosed males contain far fewer spermatids than normal, and spermatids are large and multinucleate. The size of the nebenkern is proportional to the ploidy of the spermatid. Homozygous females are wild type. In trans to a deficiency a female sterile phenotype is revealed. Ovaries are smaller than wild type and have short fused or extra dorsal appendages. Only 10% of the eggs hatch. Follicle cells in mutant ovaries have an abnormal appearance, nuclei varying considerably in size, and some of the follicle cells appear to have two nuclei. Cytokinesis fails to occur in some follicle cells.

      (Castrillon and Wasserman, 1994)

      Late-stage cysts are present at eclosion. Testes are empty in 5-day old males. Hemizygotes are male sterile and female semi-sterile. Ovaries contain a few egg chambers.

      (Castrillon et al., 1993)

      female semi-sterile. Late-stage cysts are present at eclosion. Testes empty in 5-day old males. Ovaries contain few egg chambers. sterile when heterozygous with Df(2L)TW161

      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Statement
      Reference

      dia[+]/dia1 is a suppressor of visible phenotype of LIMK1UAS.cCa, Scer\GAL4en-e16E

      (Chen et al., 2004)
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      dia[+]/dia1 is a suppressor of wing phenotype of LIMK1UAS.cCa, Scer\GAL4en-e16E

      (Chen et al., 2004)

      dia[+]/dia1 is a suppressor of mitotic domain 1 | embryonic cycle 14 phenotype of CycB+t10

      (Ji et al., 2002)
      NOT Suppressor of
      Statement
      Reference

      dia[+]/dia1 is a non-suppressor of aster | embryonic cycle 5 phenotype of CycB+t10

      (Ji et al., 2002)

      dia[+]/dia1 is a non-suppressor of aster | embryonic cycle 6 phenotype of CycB+t10

      (Ji et al., 2002)

      dia[+]/dia1 is a non-suppressor of aster | embryonic cycle 7 phenotype of CycB+t10

      (Ji et al., 2002)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      dia1/+ suppresses the mutant wing phenotype caused by expression of LIMK1Scer\UAS.cCa under the control of Scer\GAL4en-e16E (the % of wings with normal morphology at 18oC is increased from 9% to 98%).

      (Chen et al., 2004)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      dia1 is rescued by dia+t11

      (Castrillon and Wasserman, 1994)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer

      A. Spradling.

      Comments
      Comments

      Complements: Hr3802306.

      (BDGP Project Members, 1994-1999)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      dia04138
      dia1
      (Tóth et al., 2022, Jin et al., 2020)
      ms(2)04138
      (Butler, 2001, Butler et al., 2001, Beaton, 1999.12.12, Spradling et al., 1999)
      ms(2)0413804138
      Name Synonyms
      Secondary FlyBase IDs
        References (14)