FlyBase Allele Report: Dmel\Rok[cat.UAS.cVa]

General Information

Symbol

Dmel\Rok^cat.UAS.cVa

Species

D. melanogaster

Name

FlyBase ID

FBal0246224

Feature type

allele

Associated gene

Dmel\Rok

Associated Insertion(s)

Carried in Construct

P{UAS-Rok.cat.V}

Key Links

Transgenic product class

inframe_deletion

(Verdier et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Verdier et al., 2006)

Mutagen

in vitro construct

(Verdier et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-Rok.cat.V}

Cytology

Description

UASt regulatory sequences drive expression of the catalytic domain (amino acids 1-506) of Rok.

(Verdier et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Rok

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^69B

(Verdier et al., 2006)

lethal, with Scer\GAL4^Act5C.PU

(Verdier et al., 2006)

lethal, with Scer\GAL4^da.G32

(Verdier et al., 2006)

lethal, with Scer\GAL4^en-e16E

(Verdier et al., 2006)

lethal, with Scer\GAL4^insc-Mz1407

(Verdier et al., 2006)

lethal, with Scer\GAL4^LE

(Verdier et al., 2006)

lethal, with Scer\GAL4^prd.RG1

(Verdier et al., 2006)

lethal, with Scer\GAL4^Tub.PU

(Verdier et al., 2006)

partially lethal - majority die, with Scer\GAL4^elav.PU

(Verdier et al., 2006)

viable, with Scer\GAL4^Cy6

(Verdier et al., 2006)

Phenotype Manifest In

crossvein, with Scer\GAL4^en-e16E

(Verdier et al., 2006)

eye, with Scer\GAL4^69B

(Verdier et al., 2006)

eye, with Scer\GAL4^ey.PU

(Verdier et al., 2006)

eye, with Scer\GAL4^GMR.PU

(Verdier et al., 2006)

wing margin, with Scer\GAL4^Cy6

(Verdier et al., 2006)

Detailed Description

Statement

Reference

Expression of rok^cat.Scer\UAS at low levels under the control of Scer\GAL4^69B results in a rough-eye phenotype. Expression at higher levels results in lethality.

Expression of rok^cat.Scer\UAS at low levels in the posterior wing disc under the control of Scer\GAL4^en-e16E results in missing crossveins. Expression at higher levels results in lethality.

Expression of rok^cat.Scer\UAS in the wing disc margin under the control of Scer\GAL4^Cy6 results in notched-wings.

Expression of rok^cat.Scer\UAS in the eye disc under the control of Scer\GAL4^ey.PU or Scer\GAL4^GMR.PU both results in a rough-eye phenotype.

Expression of rok^cat.Scer\UAS in the embryonic nervous system under the control of Scer\GAL4^elav.PU does not affect ventral nerve cord morphology.

(Verdier et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Rok^cat.UAS.cVa, Scer\GAL4^en-e16E has crossvein phenotype, suppressible by dia[+]/dia^k07135

(Verdier et al., 2006)

Rok^cat.UAS.cVa, Scer\GAL4^en-e16E has crossvein phenotype, suppressible | partially by Rho1^72O/Rho1[+]

(Verdier et al., 2006)

Rok^cat.UAS.cVa, Scer\GAL4^en-e16E has crossvein phenotype, suppressible | partially by zip[+]/zip¹

(Verdier et al., 2006)

NOT suppressed by

Statement

Reference

Rok^cat.UAS.cVa, Scer\GAL4^en-e16E has crossvein phenotype, non-suppressible by hts[+]/hts⁰¹¹⁰³

(Verdier et al., 2006)

Rok^cat.UAS.cVa, Scer\GAL4^en-e16E has crossvein phenotype, non-suppressible by sqh²/sqh[+]

(Verdier et al., 2006)

Other

Statement

Reference

LIMK1^UAS.cCa, Rok^cat.UAS.cVa, Scer\GAL4^en-e16E has larval ventral nerve cord | embryonic stage phenotype

(Verdier et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The lack of crossveins in wings from animals expressing rok^cat.Scer\UAS under the control of Scer\GAL4^en-e16E is not suppressed in a hts⁰¹¹⁰³/+ background, but is suppressed in a dia^k07135/+ background.

Rho1^72O/+ partially suppresses the wing phenotype of animals expressing rok^cat.Scer\UAS under the control of Scer\GAL4^en-e16E.

zip¹/+ partially suppresses the wing phenotype of animals expressing rok^cat.Scer\UAS under the control of Scer\GAL4^en-e16E.

sqh²/+ does not suppress the wing phenotype of animals expressing rok^cat.Scer\UAS under the control of Scer\GAL4^en-e16E.

Co-expression of LIMK1^Scer\UAS.cCa and rok^cat.Scer\UAS in the embryonic nervous system under the control of Scer\GAL4^elav.PU results in breaks along the ventral nerve cord.

(Verdier et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Rok^cat.Scer\UAS

Rok^cat.UAS.cVa

Rok^cat.UAS

rok^cat.Scer\UAS

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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