FlyBase Allele Report: Dmel\hts[01103]

General Information

Symbol

Dmel\hts⁰¹¹⁰³

Species

D. melanogaster

Name

FlyBase ID

FBal0007962

Feature type

allele

Associated gene

Dmel\hts

Associated Insertion(s)

P{PZ}hts⁰¹¹⁰³

Carried in Construct

Also Known As

hts¹¹⁰³

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Ohler et al., 2011)

Mutagen

P-element activity

Progenitor genotype

Associated Insertion(s)

P{PZ}hts⁰¹¹⁰³

Cytology

Description

P{PZ} insertion in an intron. P{lacW} insertion in an intron.

(Palmer et al., 1999)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cerebral palsy

CEA

(Kruer et al., 2013)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Kaltenbach et al., 2007)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | progressive (with Df(2R)BSC26)

(Kruer et al., 2013)

abnormal meiotic cell cycle (with Df(2R)BSC26)

(Wilson, 2005)

abnormal neuroanatomy (with Df(2R)BSC26)

(Ohler et al., 2011)

abnormal neuroanatomy | progressive (with Df(2R)BSC26)

(Kruer et al., 2013)

abnormal neuroanatomy | third instar larval stage

(Blunk et al., 2014)

female sterile (with Df(2R)BSC26)

(Wilson, 2005)

male semi-fertile (with Df(2R)BSC135)

(Kaufman et al., 2020)

male sterile (with Df(2R)BSC26)

(Wilson, 2005)

partially lethal - majority die

partially lethal - majority die | recessive

(Spradling et al., 1999)

viable (with Df(2R)BSC26)

(Ohler et al., 2011)

Phenotype Manifest In

centrosome & spermatocyte (with Df(2R)BSC26)

(Wilson, 2005)

cortical actin cytoskeleton

(Blunk et al., 2014)

embryonic/larval neuromuscular junction | third instar larval stage

(Blunk et al., 2014)

fusome & testis (with Df(2R)BSC26)

(Wilson, 2005)

germarium (with Df(2R)BSC26)

(Hill et al., 2014)

germ cell & egg chamber (with Df(2R)BSC26)

(Wilson, 2005)

lamina | adult stage | progressive (with Df(2R)BSC26)

(Kruer et al., 2013)

male germline stem cell (with Df(2R)BSC26)

(Yuan et al., 2012)

medulla (with Df(2R)BSC26)

(Ohler et al., 2011)

medulla | adult stage | progressive (with Df(2R)BSC26)

(Kruer et al., 2013)

mitochondrion (with Df(2R)BSC26)

(Hill et al., 2014)

photoreceptor cell R7 (with Df(2R)BSC26)

(Ohler et al., 2011)

photoreceptor cell R8 (with Df(2R)BSC26)

(Ohler et al., 2011)

spermatocyte cyst (with Df(2R)BSC26)

(Wilson, 2005)

spindle & spermatocyte (with Df(2R)BSC26)

(Wilson, 2005)

Detailed Description

Statement

Reference

Phalloidin staining of hts⁰¹¹⁰³ mutant neuromuscular junctions (NMJs) in third instar larvae reveal an actin distribution distinct from wild-type. hts⁰¹¹⁰³ mutants display abnormal actin rings throughout the muscle that are not observed in wild-type.

(Blunk et al., 2014)

mtDNA replication (EdU incorporation) is completely absent in region 2b of hts⁰¹¹⁰³/Df(2R)BSC26 mutant germaria. Replication appears normal in the ovarian stem cells and post-germarium egg chambers.

(Hill et al., 2014)

hts⁰¹¹⁰³/Df(2R)BSC26 flies do not show any lesions in the lamina or medulla one day after eclosion. However, vacuoles develop in the lamina after 14 days, and extend to the medulla after 28 days. Wild type brains of this age are normal.

Fourteen-day-old hts⁰¹¹⁰³/Df(2R)BSC26 flies show severe deficits in climbing up a tube (8cm) within 8 seconds, compared to wild type.

(Kruer et al., 2013)

20 day old hts⁰¹¹⁰³/Df(2R)BSC26 males show elevated spindle misorientation in the male germline stem cells (GSCs), without a significant increase in centrosome misorientation compared to controls. Spindle misorientation is not seen in the GSCs of newly eclosed males.

(Yuan et al., 2012)

hts⁰¹¹⁰³/Df(2R)BSC26 flies show a severe disruption of the medulla. The regular array of R7 and R8 axons is lost and axons clump together forming irregularly spaced thick bundles and gaps in between. R8 and R7 axons follow disordered paths from M1 to their respective target layer and specifically R8 often overshoots its correct target layer.

Flies that are overall hts⁰¹¹⁰³/+ but have hts⁰¹¹⁰³ homozygous eyes show no obvious defect in the medulla.

(Ohler et al., 2011)

Many egg chambers from hts⁰¹¹⁰³/Df(2R)BSC26 frequently lack a full complement of germ cells.

Gonial cells of hts⁰¹¹⁰³/Df(2R)BSC26 testes do not show fusome structures by immunostaining of Klp61F.

In contrast to wild type, many spermatocytes in hts⁰¹¹⁰³/Df(2R)BSC26 mutants contain too many or too few centrosomes.

In contrast to wild type, 33% of meiotic spermatocytes in hts⁰¹¹⁰³/Df(2R)BSC26 mutant testes show spindle abnormalities, including monopolar and multipolar spindles.

While spermatocytes within a cyst proceed through meiosis in near synchrony in wild type animals, cysts of hts⁰¹¹⁰³/Df(2R)BSC26 testes harbour spermatocytes at several different stages of meiosis as well as post-meiotic spermatids.

(Wilson, 2005)

visible rough eye, pale ocelli, bent wing

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

hts[+]/hts⁰¹¹⁰³ is an enhancer of visible | adult stage phenotype of Scer\GAL4^GMR.PU, Zzzz\CGG^90.UAS.EGFP

(Qurashi et al., 2011)

Suppressor of

Statement

Reference

hts⁰¹¹⁰³ is a suppressor of visible phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^GMR.PU

(Kaltenbach et al., 2007)

hts[+]/hts⁰¹¹⁰³ is a suppressor of visible phenotype of Rok^cat.GMR

(Verdier et al., 2006)

Phenotype Manifest In

Enhancer of

Statement

Reference

hts[+]/hts⁰¹¹⁰³ is an enhancer of eye phenotype of Scer\GAL4^GMR.PU, Zzzz\CGG^90.UAS.EGFP

(Qurashi et al., 2011)

hts[+]/hts⁰¹¹⁰³ is an enhancer of ommatidium | adult stage phenotype of Scer\GAL4^GMR.PU, Zzzz\CGG^90.UAS.EGFP

(Qurashi et al., 2011)

hts[+]/hts⁰¹¹⁰³ is an enhancer of interommatidial bristle | adult stage phenotype of Scer\GAL4^GMR.PU, Zzzz\CGG^90.UAS.EGFP

(Qurashi et al., 2011)

Suppressor of

Statement

Reference

hts⁰¹¹⁰³/hts¹ is a suppressor of female fusome phenotype of Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

hts⁰¹¹⁰³/hts¹ is a suppressor of egg chamber phenotype of Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

hts⁰¹¹⁰³/hts¹ is a suppressor of ring canal phenotype of Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

hts⁰¹¹⁰³/hts¹ is a suppressor of nurse cell phenotype of Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

hts⁰¹¹⁰³/hts¹ is a suppressor of oocyte phenotype of Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

hts⁰¹¹⁰³/hts¹ is a suppressor of female germline cyst phenotype of Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

hts⁰¹¹⁰³ is a suppressor of eye phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^GMR.PU

(Kaltenbach et al., 2007)

hts[+]/hts⁰¹¹⁰³ is a suppressor of eye phenotype of Rok^cat.GMR

(Verdier et al., 2006)

NOT Suppressor of

Statement

Reference

hts[+]/hts⁰¹¹⁰³ is a non-suppressor of crossvein phenotype of Rok^cat.UAS.cVa, Scer\GAL4^en-e16E

(Verdier et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

A hts¹/hts⁰¹¹⁰³ background, which removes the fusome from ovaries, results in suppression of the synchronous additional division found in germline stem cells expressing Det^{S125E.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

(Mathieu et al., 2013)

The eye degeneration phenotype (rough eye with disorganized ommatidial lattice and interommatidial bristles) characteristic for flies expressing Zzzz\CGG^{90.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^GMR.PU is significantly worsened by combination with a single copy of hts⁰¹¹⁰³.

(Qurashi et al., 2011)

The eye phenotype of rok^cat.GMR flies is suppressed in a hts⁰¹¹⁰³/+ or dia^k07135/+ background.

The lack of crossveins in wings from animals expressing rok^cat.Scer\UAS under the control of Scer\GAL4^en-e16E is not suppressed in a hts⁰¹¹⁰³/+ background, but is suppressed in a dia^k07135/+ background.

(Verdier et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

hts⁰⁰⁶³⁴

(BDGP Project Members, 1994-1999)

hts^k06121

(BDGP Project Members, 1994-1999)

Partially rescued by

hts⁰¹¹⁰³/Df(2R)BSC26 is partially rescued by hts^GMR.Add1

(Ohler et al., 2011)

hts⁰¹¹⁰³/Df(2R)BSC26 is partially rescued by hts^GMR.PD

(Ohler et al., 2011)

Not rescued by

hts⁰¹¹⁰³/Df(2R)BSC26 is not rescued by hts^GMR.ShAdd

(Ohler et al., 2011)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

10989

cn¹ P{PZ}hts⁰¹¹⁰³/CyO; ry⁵⁰⁶

Notes on Origin

Discoverer

A. Spradling.

Comments

Complements: 18w⁰⁰⁰⁵³. Complements: sm⁰⁵³³⁸. Complements: l(2)k00705^k00705. Complements: l(2)k00705^k00806. Complements: par-1^k06323. Complements: l(2)k16207^k16207. Complements: RpL11^k16914.

(BDGP Project Members, 1994-1999)

The transposon insertion in hts⁰¹¹⁰³ is predicted to affect all hts transcripts.

(Wilson, 2005)

Based on fusome assembly and immunoblot analysis, hts mutants can be placed in an allelic series from the most to the least severe: hts⁰¹¹⁰³ > hts¹ > hts^KG06777 > hts^k06121.

(Wilson, 2005)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

01103

(Kaltenbach et al., 2007)

Hts⁰¹¹⁰³

(Hill et al., 2014, Qurashi et al., 2011)

P989

(Konsolaki and Schupbach, 1998)

hts01103

(Kruer et al., 2013)

hts⁰¹¹⁰³

(Parra et al., 2023, Qu et al., 2017, Hill et al., 2014, Wang et al., 2014, Mathieu et al., 2013, Yuan et al., 2012, Ohler et al., 2011, Wilson, 2005)

hts¹¹⁰³

(Kaufman et al., 2020, Blunk et al., 2014, Kruer et al., 2013, Pielage et al., 2011, Verdier et al., 2006)

l(2)01103⁰¹¹⁰³

l(2)k01103

(Palmer et al., 1999)

sl(2)01103

(Spradling et al., 1999)

Name Synonyms

Secondary FlyBase IDs

References (22)

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