FlyBase Allele Report: Dmel\Baldspot[02281]

General Information

Symbol

Dmel\Baldspot⁰²²⁸¹

Species

D. melanogaster

Name

FlyBase ID

FBal0009442

Feature type

allele

Associated gene

Dmel\Baldspot

Associated Insertion(s)

P{PZ}Baldspot⁰²²⁸¹

Carried in Construct

Also Known As

l(3)02281

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

Progenitor genotype

Associated Insertion(s)

P{PZ}Baldspot⁰²²⁸¹

Cytology

Description

Insertion 360bp into the first exon.

(Jung et al., 2007)

P{PZ} insertion in the first intron of Baldspot.

(Misra, 2000.11.18)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:16,661,282..16,661,282 [+]

(Senyilmaz et al., 2015, Jung et al., 2007, Misra, 2000.11.18, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body color

abnormal body color | recessive

(Berkeley Drosophila Genome Project, 1993.6.1)

lethal | recessive

(Jung et al., 2007, Spradling et al., 1999)

lethal - all die before end of larval stage

(Senyilmaz et al., 2015)

partially lethal - majority die | recessive

(Berkeley Drosophila Genome Project, 1993.6.1)

visible | recessive

(Berkeley Drosophila Genome Project, 1993.6.1)

Phenotype Manifest In

mitochondrion

(Senyilmaz et al., 2015)

Detailed Description

Statement

Reference

Baldspot⁰²²⁸¹ mutant larvae have impaired C16:0 to C18:0 (stearic acid) elongase activity and reduced C18:0 levels. Flies are able to survive to pupation if the food is supplemented with C18:0 , but not with C18.1 or C20:0 . Removing anti-fungal agents from the food also improves Baldspot⁰²²⁸¹ mutant survival. Adding sub-lethal concentrations of the mitochondrial respiratory chain complex I inhibitor rotonene (but not other drugs) to this anti-fungal food results in lethality.

Baldspot⁰²²⁸¹ mutants have impaired mitochondrial respiration and this is rescued by adding C18:0 to the food.

The number of mitochondria in Baldspot⁰²²⁸¹ mutant larvae is similar to controls, but they are hyper-fragmented. This effect is rescued by C18:0 .

Baldspot⁰²²⁸¹ mutants display reduced endoplasmic-reticulum-mitochondrial connections and abnormal cristae.

(Senyilmaz et al., 2015)

Body colour: dark.

(Berkeley Drosophila Genome Project, 1993.6.1)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Baldspot⁰²²⁸¹ has lethal phenotype, suppressible by Scer\GAL4^Act.PU/srl^UAS.cTa

(Senyilmaz et al., 2015)

Baldspot⁰²²⁸¹ has lethal - all die before end of larval stage phenotype, suppressible by Scer\GAL4^da.PU/Marf^UAS.cSa

(Senyilmaz et al., 2015)

Baldspot⁰²²⁸¹ has lethal - all die before end of larval stage phenotype, suppressible by HUWE1^GLC01617/Scer\GAL4^da.PU

(Senyilmaz et al., 2015)

Baldspot⁰²²⁸¹ has lethal phenotype, suppressible by Scer\GAL4^Act.PU/Cint\AOX^UAS.cFa

(Senyilmaz et al., 2015)

Baldspot⁰²²⁸¹ has lethal phenotype, suppressible by Scer\GAL4^Act.PU/Hsap\ELOVL6^UAS.cSa

(Senyilmaz et al., 2015)

Other

Statement

Reference

Baldspot⁰²²⁸¹, Marf^UAS.cSa, Scer\GAL4^da.PU has lethal - all die before end of pupal stage phenotype

(Senyilmaz et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Baldspot⁰²²⁸¹ has mitochondrion phenotype, suppressible by Scer\GAL4^Act.PU/Cint\AOX^UAS.cFa

(Senyilmaz et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of srl^Scer\UAS.cTa under the control of Scer\GAL4^Act.PU suppresses the lethality seen in Baldspot⁰²²⁸¹ mutants.

Expression of Marf^Scer\UAS.cSa under the control of Scer\GAL4^da.PU rescues the lethality seen in Baldspot⁰²²⁸¹ mutants until pupation.

Expression of CG8184^GLC01617 under the control of Scer\GAL4^da.PU suppresses the larval lethality seen in Baldspot⁰²²⁸¹ mutants. The number of pupae seen in similar to controls.

(Senyilmaz et al., 2015)

Xenogenetic Interactions

Statement

Reference

Expression of Zzzz\AOX^Scer\UAS.cFa under the control of Scer\GAL4^Act.PU suppresses the lethality and defects in mitochondrial respiration seen in Baldspot⁰²²⁸¹ mutants.

Expression of Hsap\ELOVL6^Scer\UAS.cSa under the control of Scer\GAL4^Act.PU rescues the lethality seen in Baldspot⁰²²⁸¹ mutants.

(Senyilmaz et al., 2015)

Complementation and Rescue Data

Complements

Baldspot^neo21

(BDGP Project Members, 1994-1999)

Fails to complement

Baldspot^neo22

(BDGP Project Members, 1994-1999)

Baldspot⁰⁶⁵¹⁹

(BDGP Project Members, 1994-1999)

Baldspot⁰⁴¹⁰⁶

(BDGP Project Members, 1994-1999)

Baldspot⁰³²³⁴

(BDGP Project Members, 1994-1999)

Baldspot⁰³²²⁹

(BDGP Project Members, 1994-1999)

Baldspot⁰¹⁸⁹⁵

(BDGP Project Members, 1994-1999)

Rescued by

Baldspot⁰²²⁸¹ is rescued by Baldspot^cJa

(Jung et al., 2007)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

11563

P{PZ}Baldspot⁰²²⁸¹ ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹

Notes on Origin

Discoverer

A. Spradling.

Separable from: P{PZ}02281b.

(Jung et al., 2007)

The "l(3)02281" line contains two insertions - the P{PZ}Baldspot⁰²²⁸¹ insertion in Baldspot and the P{PZ}02281b insertion which is located in the short intergenic region between E(bx) and mthl14.

(Jung et al., 2007)

Comments

Complements: Mo25⁰⁰²⁷⁴. Complements: l(3)neo20¹.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Baldspot⁰²²⁸¹

l(3)02281

(Jung et al., 2007, Misra, 2000.11.18, Spradling et al., 1999)

l(3)02281⁰²²⁸¹

l(3)neo22⁰²²⁸¹

(BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project, 1993.6.1)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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