FB2024_03 , released June 25, 2024
Allele: Dmel\okrWS
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General Information
Symbol
Dmel\okrWS
Species
D. melanogaster
Name
FlyBase ID
FBal0013237
Feature type
allele
Associated gene
Dmel\okr
Associated Insertion(s)
Carried in Construct
Also Known As
okrWS1
Key Links
Genomic Maps

Allele class

antimorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

antimorphic allele - genetic evidence

(Ghabrial et al., 1998)
Mutagen
ethyl methanesulfonate
(Schupbach and Wieschaus, 1991)
Progenitor genotype
Cytology
Description

Amino acid replacement: G579D.

(Ghabrial et al., 1998)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2L:2,990,803..2,990,803 [-]
Nucleotide change:

G2990803A

Amino acid change:

G579D | okr-PA

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Ghabrial et al., 1998)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Homozygous females show a high frequency (more than 50%) of region 3 cysts with two pro-oocytes (as assayed by c(3)G staining) compared to a frequency of only 9.5% in wild type.

      (Joyce and McKim, 2009)

      okrWS/Df(2L)JS17 mutants have a similar frequency of single-strand annealing repair (SSA) compared to controls in a P{wIw.FRT} hemizygous assay to study DNA double-stranded break repair when assayed at 38oC, but show a higher frequency of SSA than controls in the same assay at 32oC.

      okrWS/Df(2L)JS17 mutants have an increased SSA frequency compared to controls in a P{wIw.FRT}/P{wIw.FRT.8z} homozygous assay to study DNA double-stranded break repair when assayed at 38oC.

      (Wei and Rong, 2007)

      Hemizygotes are viable. Hemizygous larvae are sensitive to methyl methanesulfonate (MMS) compared to wild-type larvae, showing a significant reduction in survival after being fed with a 0.08% solution of MMS.

      (Ghabrial et al., 1998)

      Most extreme eggs are spindle shaped without dorsal appendages. Other eggs have fused dorsal appendages or of normal morphology but remain unfertilized. Eggs often have a second micropyle at the posterior end.

      (Schupbach and Wieschaus, 1991)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT suppressed by
      Statement
      Reference

      okrWS has sterile phenotype, non-suppressible by c(2)MZ0810

      (Manheim and McKim, 2003)
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      okrWS has presumptive oocyte phenotype, suppressible by pch2EY01788a

      (Joyce and McKim, 2009)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The high frequency of region 3 cysts containing two pro-oocytes that is seen in okrWS homozygous females is suppressed by pch2EY01788a.

      (Joyce and McKim, 2009)

      Mutation does not suppress the bicaudal phenotype of oskbcd.3UTR.T:Zzzz\His6.

      (Wilson et al., 1996)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      References (12)