FlyBase Allele Report: Dmel\br[rbp-1]

General Information

Symbol

Dmel\br^rbp-1

Species

D. melanogaster

Name

FlyBase ID

FBal0014441

Feature type

allele

Associated gene

Dmel\br

Associated Insertion(s)

Carried in Construct

Also Known As

rbp¹, rbp^t99

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Bayer et al., 1997, Crossgrove et al., 1996, Restifo and Merrill, 1994, Kiss et al., 1988)

Mutagen

ethyl methanesulfonate

(Bayer et al., 1997, Restifo and Merrill, 1994, Kiss et al., 1988, Belyaeva et al., 1982)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult brain

(Restifo and White, 1991)

adult optic lobe

(Restifo and White, 1991)

coxal tergal remotor muscle cell

(Sandstrom and Restifo, 1999, Sandstrom et al., 1997)

dorsal appendage

(Tzolovsky et al., 1999)

dorsal appendage (with br¹)

(Tzolovsky et al., 1999)

dorsal longitudinal indirect flight muscle cell

(Sandstrom et al., 1997)

egg chorion

(Tzolovsky et al., 1999)

embryonic/larval proventriculus | embryonic stage

(Restifo and Merrill, 1994, Restifo and White, 1992)

embryonic/larval salivary gland | embryonic stage

(Restifo and Merrill, 1994, Restifo and White, 1992)

indirect flight muscle cell

(Restifo and White, 1992)

lateral oblique dorsal muscle cell

(Sandstrom and Restifo, 1999, Sandstrom et al., 1997)

male genitalia

(Wilson et al., 2006)

male genitalia (with Dp(1;Y)Sz280)

(Wilson et al., 2006)

maxillary palp bristle

(Belyaeva et al., 1980)

muscle tendon junction & coxal tergal remotor muscle

(Sandstrom and Restifo, 1999)

muscle tendon junction & lateral oblique dorsal muscle

(Sandstrom and Restifo, 1999)

muscle tendon junction & tergosternal muscle

(Sandstrom and Restifo, 1999)

ovary

(Tzolovsky et al., 1999)

tendon cell

(Sandstrom and Restifo, 1999)

tergosternal muscle cell

(Sandstrom and Restifo, 1999, Sandstrom et al., 1997)

tergotrochanter muscle cell

(Sandstrom et al., 1997)

thorax

(Kiss et al., 1988)

Detailed Description

Statement

Reference

100% of br^rbp-1/Y and br^rbp-1/Dp(1;Y)Sz280 males have malrotated genitalia. This phenotype is rescued by the Dp(1;Y)y²67g19.1 duplication.

(Wilson et al., 2006)

br^rbp-1 flies do not survive to eclosion. 100% reach pupal stage stage P6, 84% reach stage P12, and 80% become pharate adults.

(Bayer et al., 2003)

The dorsoventral indirect flight muscles (DVMs) of mutant flies are still attached to the dorsal epidermis in pupae 14 hours after head eversion, but the connecting material is sparse. The dorsal ends of the muscles are narrower than in wild type, while the ventral ends appear normal. The dorsal myotendinous junction (MTJ) is defective, with the phenotype varying in severity. The area of muscle-tendon contact is reduced in mildly affected cases, although the muscle and tendon cell membranes do interdigitate and form spot adherens junctions. The number of tendon cell process extending from the dorsal epidermal muscle attachment site (EMA) is reduced. At the most aberrant MTJs, small numbers of tendon cell processes are tenuously linked to the muscle by strands of material that may be extracellular matrix or cellular debris. The sheath that surrounds the sparse and defective tendon processes is aberrant, with numerous gaps, especially along the medial side. The ventral MTJs appear normal at this stage.

(Sandstrom and Restifo, 1999)

br¹/br^rbp-1 mothers produce eggs that have reduced dorsal appendages, Eggs dissected from homozygous br^rbp-1 females have dorsal appendages that are shorter than wild-type and irregular in shape, and the eggshell is fragile. br^rbp-1/br^npr-6 females produce very few offspring.

(Tzolovsky et al., 1999)

Homozygotes arrest development at the early pupal or pharate adult stages.

(Bayer et al., 1997)

DVM are virtually eliminated (a deficit in thoracic muscle precursors). DLM fibres often remain fused at their ends rather than splitting from three primordia into six DLM fibres. DLM, TTM and the few surviving DVM fibres occupy incorrect attachment sites on the dorsal thorax at epidermal locations normally occupied by other muscle fibres.

(Sandstrom et al., 1997)

Heterozygotes with br^rbp-4 have the salivary gland tangled in the head capsule and an abnormal proventriculus structure.

(Restifo and Merrill, 1994)

Failure of salivary gland degeneration. Reduction in dorso-ventral class of indirect flight muscles. Proventriculus abnormality: foregut-midgut junction less proventriculus-like in appearance.

(Restifo and White, 1992)

Intermoult gene transcriptional phenotypes.

(Guay and Guild, 1991)

70% penetrance of abnormal SEG-TG separation phenotype, 84% with an abnormal optic lobe position and 51% with brain fusion failure in rbp mutants.

(Restifo and White, 1991)

Thorax and appendages are slightly shorter or smaller than normal.

(Kiss et al., 1988)

Pupae die with developed imaginal organs.

(Belyaeva et al., 1982)

Heterozygotes with Df(1)br²⁵ have normal viability and reduced bristle number on the palpus.

(Belyaeva et al., 1980)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

br^rbp-1 has lethal phenotype, enhanceable by Met²⁷/Rst(1)JH[+]

(Wilson et al., 2006)

Suppressor of

Statement

Reference

br^rbp-1 is a suppressor of lethal | pupal stage phenotype of Msex\br^Z4.hs

(Bayer et al., 2003)

Other

Statement

Reference

Met²⁷, br^rbp-1/br⁵ has lethal phenotype

(Wilson et al., 2006)

Met²⁷, br^rbp-1/br^2Bc-1 has lethal phenotype

(Wilson et al., 2006)

Met²⁷/Met[+], br^rbp-1 has lethal - all die during P-stage phenotype

(Wilson et al., 2006)

Met¹²⁸, br^rbp-1/br¹ has lethal phenotype

(Wilson et al., 2006)

Met²⁷, br^rbp-2/br^rbp-1 has lethal - all die during P-stage phenotype

(Wilson et al., 2006)

Phenotype Manifest In

Suppressor of

Statement

Reference

br^rbp-1 is a suppressor of leg phenotype of Msex\br^Z4.hs

(Bayer et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

br^rbp-1/br^rbp-1, Rst(1)JH²⁷/+ double mutants die at an earlier stage (prepupal/early pupal) than br^rbp-1/br^rbp-1 mutants. br^rbp-2/br^rbp-1, Rst(1)JH²⁷/Rst(1)JH²⁷ flies are also prepupal/early pupal lethals. Expression of the Rst(1)JH^+tSt-H.hs transgene can rescue the lethality of these flies.

(Wilson et al., 2006)

Xenogenetic Interactions

Statement

Reference

When one copy of the Msex\br^Z4.hs transgene is expressed in br^rbp-1 mutant flies, only 1.5% of br^rbp-1 mutants are 'rescued' to eclosion. Flies carrying two doses of Msex\br^Z4.hs experience approximately 8% rescue to eclosion. Mutant br^rbp-1 flies rescued with Msex\br^Z4.hs typically die within 1-2 days of eclosion. These animals have a protracted proboscis and are most likely unable to feed. The malformed leg phenotype found in br^rbp-1 animals that develop to late pupal stages is rescued in approximately 66% of cases by the expression of two Msex\br^Z4.hs transgenes.

(Bayer et al., 2003)

Complementation and Rescue Data

Fails to complement

br^npr-6

(Tzolovsky et al., 1999)

Rescued by

br^rbp-1 is rescued by br^{BRcore.TNT.Q1.Z1.hs}

(Bayer et al., 2003)

br^rbp-1 is rescued by br^{BRcore.TNT.Q1.Z1.hs}

(Bayer et al., 1997)

br^rbp-1 is rescued by br^{BRcore.Q1.Z1.hs}

(Bayer et al., 1997)

br^rbp-1 is rescued by br^{BRcore.TNT.Q1.Z1.hs}

(Sandstrom et al., 1997)

Partially rescued by

br^rbp-1 is partially rescued by br^BRcore.Z4.hs

(Bayer et al., 2003)

br^rbp-1 is partially rescued by br^BRcore.Z4.hs

(Bayer et al., 1997)

br^rbp-1 is partially rescued by br^BRcore.Z4.hs

(Sandstrom et al., 1997)

Not rescued by

br^rbp-1 is not rescued by br^Z2.hs

(Bayer et al., 1997)

br^rbp-1 is not rescued by br^{BRcore.NS.Z3.hs}

(Bayer et al., 1997)

br^rbp-1 is not rescued by br^Z2.hs

(Sandstrom et al., 1997)

br^rbp-1 is not rescued by br^{BRcore.NS.Z3.hs}

(Sandstrom et al., 1997)

Comments

Significant rescue of br^rbp-1 mutant flies is observed with one copy of the br^{BRcore.TNT.Q1.Z1.hs} transgene (64% survive to eclosion). Only marginal improvement in rescue (69% compared to 64%) is observed when two copies of br^{BRcore.TNT.Q1.Z1.hs} are used. The majority of the rescued animals have normal legs.

Expression of one copy of br^BRcore.Z4.hs in br^rbp-1 mutant flies results rescue of only 7% of animals to eclosion. This increases to 13% when two copies are used. Mutant br^rbp-1 flies rescued with br^BRcore.Z4.hs typically die within 1-2 days of eclosion. These animals have a protracted proboscis and are most likely unable to feed. The malformed leg phenotype found in br^rbp-1 animals that develop to late pupal stages is rescued in approximately 50% of cases by the expression of two br^BRcore.Z4.hs transgenes.

(Bayer et al., 2003)

br^rbp-1/+ x br^npr-6/+ crosses lead to very few transheterozygous males but no females being produced. Offspring die as pupae. Complementation is based on lethality.

(Tzolovsky et al., 1999)

Over 50% of br^rbp-1/Y males carrying two copies of either br^{BRcore.Q1.Z1.hs} or br^{BRcore.TNT.Q1.Z1.hs} are rescued to eclosion when br^{BRcore.Q1.Z1.hs} or br^{BRcore.TNT.Q1.Z1.hs} is expressed using a 33^oC heat shock in 6 hour prepupae followed by a 37^oC heat shock just after head emergence. The rescued animals are completely wild-type. br^BRcore.Z4.hs partially rescues br^rbp-1/Y males; 31% of br^rbp-1/Y males carrying br^BRcore.Z4.hs eclose when br^BRcore.Z4.hs expression is induced in prepupae and pupae. The rescued flies have the br^rbp-1 reduced bristle phenotype and are not fertile. br^Z2.hs and br^{BRcore.NS.Z3.hs} do not rescue br^rbp-1/Y males.

(Bayer et al., 1997)

Heat induced expression of P{hs-BRC-Z1} or P{hs-BRC-Z4} around the time of head eversion (approximately 12 hours after puparium formation) partially rescues the lethality. Heat induced expression of P{hs-BRC-Z1} completely rescues the DVM, expression of P{hs-BRC-Z4} only partially rescues and expression of P{hs-BRC-Z2} and P{hs-BRC-Z3} fail to rescue the DVM phenotype.

(Sandstrom et al., 1997)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

br^rbp-1

lt99

rbp¹

(Wilson et al., 2006, Bayer et al., 2003, Sandstrom and Restifo, 1999, Tzolovsky et al., 1999, Bayer et al., 1997, Sandstrom et al., 1997, Crossgrove et al., 1996, Dubrovsky et al., 1996)

rbp^t99

(Mazina et al., 1991, Mazina et al., 1990, Solovyeva and Belyaeva, 1989, Aizenzon and Belyaeva, 1982, Belyaeva et al., 1980)

t99

(Belyaeva et al., 1982)

Name Synonyms

Secondary FlyBase IDs

References (21)

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