FlyBase Allele Report: Dmel\br[5]

General Information

Symbol

Dmel\br⁵

Species

D. melanogaster

Name

FlyBase ID

FBal0001192

Feature type

allele

Associated gene

Dmel\br

Associated Insertion(s)

Carried in Construct

Also Known As

lt35, br^lt35

Key Links

Allele class

amorphic allele - genetic evidence

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Choi et al., 2006, Bayer et al., 1997, O'Keefe et al., 1995, Gotwals and Fristrom, 1991, Kiss et al., 1988)

loss of function allele

(Liu and Restifo, 1998)

Mutagen

ethyl methanesulfonate

(Bayer et al., 1997, O'Keefe et al., 1995, Gotwals and Fristrom, 1991, Kiss et al., 1988, Belyaeva et al., 1982)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult brain

(Restifo and White, 1991)

adult epidermis

(Fletcher and Thummel, 1995)

adult head

(Fletcher and Thummel, 1995)

adult optic lobe

(Restifo and White, 1991)

embryonic/larval proventriculus | embryonic stage

(Restifo and White, 1992)

embryonic/larval salivary gland | embryonic stage

(Restifo and White, 1992)

eye | somatic clone

(Brennan et al., 2001)

eye disc morphogenetic furrow

(Brennan et al., 2001)

eye disc morphogenetic furrow | somatic clone

(Brennan et al., 2001)

indirect flight muscle cell

(Restifo and White, 1992)

leg

(Ward et al., 2003)

leg disc

(Ward et al., 2003)

male genitalia, with br^Z2.hs

(Wilson et al., 2006)

ommatidium

(Brennan et al., 2001)

ommatidium | somatic clone

(Brennan et al., 2001)

photoreceptor cell R8 | somatic clone | increased number

(Brennan et al., 2001)

photoreceptor neuron | somatic clone

(Brennan et al., 2001)

photoreceptor neuron | somatic clone | increased number

(Brennan et al., 2001)

puparium cuticle

(Fletcher and Thummel, 1995)

Detailed Description

Statement

Reference

Partial rescue of br⁵/Y mutants, achieved by driving expression of the br^Z2.hs transgene by three heat shocks at 37^oC starting in the third instar results in small numbers of pharate adults, 93% of which have malrotated genitalia. br⁵/Dp(1;Y)y²67g19.1 males, exposed to the same heat shock protocol only show genitalia malrotation in 4% of cases.

(Wilson et al., 2006)

Mutant pupae show no eversion or elongation of leg imaginal discs. The leg discs move around slowly in these animals but show no developmental changes. these animals undergo a series of contractions beginning at 16 hours post puparium formation and increasing in intensity between 18 and 19 hours that appear to reflect an attempt at head eversion. One leg imaginal disc is often forced away from the others by these contractions.

(Ward et al., 2003)

br⁵/Y eye discs show a dramatic failure of furrow progression as well as ommatidial disorganisation. Homozygous clones in the eye disc show defects in ommatidial organisation, including the wrong number of photoreceptors in clusters. Excess numbers of R8 cells in a cluster are seen. Homozygous clones spanning the morphogenetic furrow are frequently associated with some slowing or furrow progression. Clones at the posterior margin of the disc do not show any visible defects in furrow initiation. Homozygous clones at the anterior margin of the adult eye show a small nick and subtle disordering of the ommatidial array.

(Brennan et al., 2001)

The br⁵/br^npr-6 heteroallelic combination is third larval instar lethal.

(Tzolovsky et al., 1999)

Homozygotes arrest development in 0-hour prepupae with an untanned larval cuticle and imaginal discs are not everted. 29% of br⁵/Y males carrying two copies of br^Z2.hs are rescued to eclosion when br^Z2.hs is expressed using 37^oC heat shocks twice during the third larval instar stage and once during pupariation. br⁵/Y males carrying two copies of br^Z2.hs can advance to prepupal or pupal stages when br^Z2.hs is expressed using 33^oC heat shocks. br^{BRcore.Q1.Z1.hs}, br^{BRcore.TNT.Q1.Z1.hs}, br^{BRcore.NS.Z3.hs} and br^BRcore.Z4.hs does not rescue the lethality of br⁵/Y males when expressed using heat shocks of either 33^oC or 37^oC.

(Bayer et al., 1997)

Homozygotes die as early prepupae.

(D'Avino et al., 1995)

Mutant males form a soft, untanned puparium, but fail to develop beyond the prepupal stage.

(Fletcher and Thummel, 1995)

Failure of salivary gland degeneration. Reduction in dorso-ventral class of indirect flight muscles. Some show proventriculus abnormality: foregut-midgut junction less proventriculus-like in appearance.

(Restifo and White, 1992)

41% penetrance of abnormal SEG-TG separation phenotype, 55% of individuals show an abnormal optic lobe position and 35% a brain fusion failure.

(Restifo and White, 1991)

Pupariation is delayed by 3 hours. The puparium is weakly pigmented.

(Belyaeva et al., 1982)

Heterozygotes with Df(1)br²⁵ are completely lethal.

(Belyaeva et al., 1980)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

br⁵/br[+] is an enhancer of visible | dominant phenotype of Bar¹

(Brennan et al., 2001)

Other

Statement

Reference

Met²⁷, br^rbp-1/br⁵ has lethal phenotype

(Wilson et al., 2006)

Met²⁷, br^rbp-2/br⁵ has lethal phenotype

(Wilson et al., 2006)

Met²⁷, br⁵/br^2Bc-1 has partially lethal phenotype

(Wilson et al., 2006)

Met²⁷, br⁵/br¹ has lethal - all die during P-stage phenotype

(Wilson et al., 2006)

E(br)121^Ebr121, br⁵/br[+] has visible | dominant phenotype

(Ward et al., 2003)

Rho1^Ebr233, br⁵/br[+] has visible | dominant phenotype

(Ward et al., 2003)

Rho1^Ebr246, br⁵/br[+] has visible | dominant phenotype

(Ward et al., 2003)

Sb^Ebr448, br⁵/br[+] has visible | dominant phenotype

(Ward et al., 2003)

E(br)333^Ebr333, br⁵/br[+] has visible | dominant phenotype

(Ward et al., 2003)

Sb^Ebr48, br⁵/br[+] has visible | dominant phenotype

(Ward et al., 2003)

E(br)121^Ebr121/E(br)121[+], br⁵ has visible phenotype

(Ward et al., 2003)

E(br)333[+]/E(br)333^Ebr333, br⁵ has visible phenotype

(Ward et al., 2003)

E(br)444[+]/E(br)444^Ebr444, br⁵ has visible phenotype

(Ward et al., 2003)

E(br)65^Ebr65/E(br)65[+], br⁵ has visible phenotype

(Ward et al., 2003)

Rho1^Ebr233/Rho1[+], br⁵ has visible phenotype

(Ward et al., 2003)

Rho1[+]/Rho1^Ebr246, br⁵ has visible phenotype

(Ward et al., 2003)

Sb[+]/Sb^Ebr448, br⁵ has visible phenotype

(Ward et al., 2003)

Sb[+]/Sb^Ebr48, br⁵ has visible phenotype

(Ward et al., 2003)

br⁵, bs[+]/bs^Ebr292 has visible phenotype

(Ward et al., 2003)

Df(3R)DG2/+, br⁵ has visible | dominant phenotype

(Ward et al., 2003)

E(br)444^Ebr444, br⁵/br[+] has visible | dominant phenotype

(Ward et al., 2003)

br⁵/br[+], bs^Ebr292 has visible | dominant phenotype

(Ward et al., 2003)

E(br)65^Ebr65, br⁵/br[+] has visible | dominant phenotype

(Ward et al., 2003)

Eip74EF^DL-1, br⁵ has lethal | recessive | larval stage phenotype

(Fletcher and Thummel, 1995)

Phenotype Manifest In

Enhancer of

Statement

Reference

br⁵/br[+] is an enhancer of eye phenotype of Bar¹

(Brennan et al., 2001)

Other

Statement

Reference

E(br)121^Ebr121, br⁵/br[+] has leg phenotype

(Ward et al., 2003)

Rho1^Ebr233, br⁵/br[+] has leg phenotype

(Ward et al., 2003)

Rho1^Ebr246, br⁵/br[+] has leg phenotype

(Ward et al., 2003)

Sb^Ebr448, br⁵/br[+] has leg phenotype

(Ward et al., 2003)

Sb^Ebr48, br⁵/br[+] has leg phenotype

(Ward et al., 2003)

E(br)333^Ebr333, br⁵/br[+] has leg phenotype

(Ward et al., 2003)

Df(3R)DG2/+, br⁵ has leg phenotype

(Ward et al., 2003)

E(br)121^Ebr121, br⁵ has leg phenotype

(Ward et al., 2003)

E(br)333^Ebr333, br⁵ has leg phenotype

(Ward et al., 2003)

E(br)444^Ebr444, br⁵ has leg phenotype

(Ward et al., 2003)

E(br)65^Ebr65, br⁵ has leg phenotype

(Ward et al., 2003)

Rho1^Ebr233, br⁵ has leg phenotype

(Ward et al., 2003)

Rho1^Ebr246, br⁵ has leg phenotype

(Ward et al., 2003)

Sb^Ebr448, br⁵ has leg phenotype

(Ward et al., 2003)

Sb^Ebr48, br⁵ has leg phenotype

(Ward et al., 2003)

br⁵, bs^Ebr292 has leg phenotype

(Ward et al., 2003)

br⁵/br[+], bs^Ebr292 has leg phenotype

(Ward et al., 2003)

E(br)444^Ebr444, br⁵/br[+] has leg phenotype

(Ward et al., 2003)

E(br)65^Ebr65, br⁵/br[+] has leg phenotype

(Ward et al., 2003)

Eip74EF^neo24, br⁵ has adult head phenotype

(Fletcher and Thummel, 1995)

Eip74EF^neo24, br⁵ has leg phenotype

(Fletcher and Thummel, 1995)

Eip74EF^neo24, br⁵ has wing phenotype

(Fletcher and Thummel, 1995)

Additional Comments

Genetic Interactions

Statement

Reference

Like br⁵ single mutants, br⁵, Rst(1)JH²⁷ double mutants are lethal in prepupal/early pupal development. 54% of br⁵/br¹, Rst(1)JH²⁷/+ mutants survive to adulthood but br⁵/br¹, Rst(1)JH²⁷/Rst(1)JH²⁷ mutants are lethal in prepupal/early pupal development.

(Wilson et al., 2006)

Double heterozygotes with Eip74EF^neo24 or Eip74EF^DL-1 show no effect on development. 58% of Eip74EF^neo24 mutants carrying a single effective dose of br⁺ arrest during the prepupal period. 20% of these prepupae show a misshapen puparium. A novel microcephalic phenotype results from partial head eversion, though eyes do develop. Leg and wing structures are absent. Lethal phenotype is more severe than either Eip74EF^neo24 or br⁵ alone. All br⁵, Eip74EF^DL-1 double mutants arrest development at the larval/prepupal transition, whereas Eip74EF^DL-1 mutants show a range of lethal phases from prepupal to pharate adult.

(Fletcher and Thummel, 1995)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

br^npr-6

(Tzolovsky et al., 1999)

Partially rescued by

br⁵ is partially rescued by br^Z2.hs

(Bayer et al., 1997)

Not rescued by

br⁵ is not rescued by br^BRcore.Z4.hs

(Bayer et al., 1997)

br⁵ is not rescued by br^{BRcore.Q1.Z1.hs}

(Bayer et al., 1997)

br⁵ is not rescued by br^{BRcore.NS.Z3.hs}

(Bayer et al., 1997)

br⁵ is not rescued by br^{BRcore.TNT.Q1.Z1.hs}

(Bayer et al., 1997)

Comments

Complementation is based on lethality.

(Tzolovsky et al., 1999)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

29972

y¹ br⁵/Binsn

Notes on Origin

Discoverer

Comments

Deficient in 'rbp' function. Imaginal discs remain as vesicles.

(Kiss et al., 1988)

zip interacts with the br^2Bc-1/br⁵.

(Gotwals and Fristrom, 1991)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

br⁵

(Choi et al., 2006)

br^lt35

(Mazina et al., 1990, Solovyeva and Belyaeva, 1989, Aizenzon and Belyaeva, 1982)

broad⁵

(Eliazer et al., 2014)

l(1)t35

lt35

(Mazina et al., 1991, Belyaeva et al., 1982, Belyaeva et al., 1980)

Name Synonyms

Secondary FlyBase IDs

References (35)

Report Sections

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