FlyBase Allele Report: Dmel\rdgA[3]

General Information

Symbol

Dmel\rdgA³

Species

D. melanogaster

Name

FlyBase ID

FBal0014459

Feature type

allele

Associated gene

Dmel\rdgA

Associated Insertion(s)

Carried in Construct

Also Known As

rdgA^PC47

Key Links

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Masai et al., 1997, Masai et al., 1993)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology

(Johnson et al., 1982)

abnormal neurophysiology (with rdgA¹)

(Kain et al., 2008)

abnormal neurophysiology | dominant

(Garcia-Murillas et al., 2006)

Phenotype Manifest In

Detailed Description

Statement

Reference

The electroantennograms of homozygous and rdgA¹/rdgA³ animals show a significant decrease in amplitude compared to controls in response to a number of odours (ethyl acetate, butanol, propionic acid, benzaldehyde and iso-amyl acetate), whereas the response of heterozygotes is close to normal.

Mutants have normal antennal morphology even 6 days after eclosion.

(Kain et al., 2008)

Photoreceptors in the eye appear ultrastructurally normal in mutant flies on eclosion, but degenerate over time.

rdgA³ flies have an abnormal electroretinogram, showing a pronounced decrease in the rate of deactivation of the light response following a 1 second stimulus of light. Photoreceptors in rdgA³/+ flies also show a small but significant defect in deactivation of the light response following a 1 second stimulus of light.

(Garcia-Murillas et al., 2006)

Newly eclosed flies have a near-wild type ommatidium morphology, with all seven rhabdomeres being distinctly visible. Progressive degeneration is seen in all six outer rhabdomeres ver time, so that at 25[o]C, but 72 hours after eclosion, only the central R7 ommatidium can be seen. Raising flies in complete darkness throughout development does not suppress the retinal degeneration. However, the rate of degeneration is significantly higher in flies grown on a 12 hours light-dark cycle compared to flies grown in complete darkness.

(Georgiev et al., 2005)

Robust light responses can be recorded from mutant photoreceptors, but quantum bumps can not be detected.

(Hardie et al., 2002)

Photoreceptor cells undergo severe degeneration in homozygous flies.

(Masai et al., 1997)

Rhabdomeres and photoreceptor cell bodies degenerate gradually after eclosion. Retinal degeneration starts with the disappearance of SRC. Ocellar photoreceptors also degenerate and degeneration of the SRC is observed.

(Yoon et al., 1996)

Homozygous larvae are negatively photokinetic with a stimulus indice significantly higher than wild type control.

(Gordesky-Gold et al., 1995)

Shows abnormal localisation of anti-RDGA protein in adult photoreceptors.

(Suzuki and Hirosawa, 1994)

The retinula cell somata and the synaptic plexus of the ocelli are normal in newly emerged flies. Extracellular debris accumulates under the ocellar retina in aged flies.

(Stark et al., 1989)

Flies show the normal arrangement of photoreceptors on the first day after pupal emergence, but by 7 days after eclosion the R1-R6 cells are noticeably disoriented (the R7 and R8 cells are less affected). The lamina and medulla appear to degenerate less than the retina. The electroretinogram (ERG) R1-R6 waveform is smaller than wild-type in a rdgA³ fly aged 6 hours, and is unrecordable in a rdgA³ fly aged 72 hours. The R7/R8 ERG waveform does not alter much from pupal emergence to 72 hours after eclosion. The M-potential and on-transient is normal in a newly emerged rdgA³ fly, although neither can be elicited in flies aged 72 hours. The prolonged polarising afterpotential is transient in rdgA³ flies aged 6 hours. The ocellar ERG decreases in older flies. Phototaxis due to R7/R8 function is impaired.

(Johnson et al., 1982)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

rdgA³ has abnormal neurophysiology | dominant phenotype, enhanceable by Scer\GAL4^ninaE.PT/laza^UAS.GFP

(Garcia-Murillas et al., 2006)

Suppressed by

Statement

Reference

rdgA³ has abnormal neurophysiology phenotype, suppressible by Galpha49B[+]/Gαq^221c

(Kain et al., 2008)

rdgA³ has abnormal neurophysiology phenotype, suppressible by Gα49B[+]/Gαq¹³⁷⁰

(Kain et al., 2008)

rdgA³ has abnormal neurophysiology phenotype, suppressible by Gαq^RNAi.UAS.1f1/Scer\GAL4^Gαq.PS

(Kain et al., 2008)

rdgA³ has abnormal neurophysiology phenotype, suppressible by laza²²

(Garcia-Murillas et al., 2006)

Suppressor of

Statement

Reference

rdgA³ is a suppressor of abnormal neurophysiology phenotype of Gαq¹

(Hardie et al., 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

rdgA³ has eye photoreceptor cell phenotype, enhanceable by Scer\GAL4^ninaE.PT/laza^UAS.GFP

(Garcia-Murillas et al., 2006)

rdgA³ has eye photoreceptor cell phenotype, enhanceable by wun^UAS.cZa/Scer\GAL4^ninaE.PT

(Garcia-Murillas et al., 2006)

rdgA³ has eye photoreceptor cell phenotype, enhanceable by wun2^UAS.cHa/Scer\GAL4^ninaE.PT

(Garcia-Murillas et al., 2006)

rdgA³ has eye photoreceptor cell phenotype, enhanceable by CG11426^UAS.GFP/Scer\GAL4^ninaE.PT

(Garcia-Murillas et al., 2006)

rdgA³ has retina phenotype, enhanceable by Scer\GAL4^ninaE.PT/laza^UAS.GFP

(Garcia-Murillas et al., 2006)

rdgA³ has eye photoreceptor cell phenotype, enhanceable by Cds¹

(Garcia-Murillas et al., 2006)

rdgA³ has eye photoreceptor cell phenotype, enhanceable by sktl^Δ1-1/sktl^Δ20

(Garcia-Murillas et al., 2006)

Suppressed by

Statement

Reference

rdgA³ has phenotype, suppressible by su(rdgA)40⁴⁰

(Georgiev et al., 2012)

rdgA³ has eye photoreceptor cell phenotype, suppressible by laza²²/laza²²

(Garcia-Murillas et al., 2006)

rdgA³ has eye photoreceptor cell phenotype, suppressible by laza[+]/laza²²

(Garcia-Murillas et al., 2006)

rdgA³ has retina phenotype, suppressible by laza²²

(Garcia-Murillas et al., 2006)

rdgA³ has rhabdomere phenotype, suppressible by norpA^P24

(Georgiev et al., 2005)

rdgA³ has rhabdomere phenotype, suppressible by Gαq¹

(Georgiev et al., 2005)

NOT suppressed by

Statement

Reference

rdgA³ has eye photoreceptor cell phenotype, non-suppressible by wun^k16806/wun^k12382

(Garcia-Murillas et al., 2006)

rdgA³ has eye photoreceptor cell phenotype, non-suppressible by wun2^EP2650ex34

(Garcia-Murillas et al., 2006)

rdgA³ has photoreceptor neuron phenotype, non-suppressible by inaC⁵

(Masai et al., 1997)

Enhancer of

Statement

Reference

rdgA³ is an enhancer of eye photoreceptor cell phenotype of Cds¹

(Garcia-Murillas et al., 2006)

Suppressor of

Statement

Reference

rdgA³ is a suppressor of photoreceptor neuron phenotype of Gαq¹

(Hardie et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Introduction of a single copy of Gα49B^221c or Gα49B¹³⁷⁰ to rdgA³ homozygotes significantly rescues the electroantennogram responses of the homozygotes.

Expression of Gα49B^{dsRNA.Scer\UAS.1f1} under the control of Scer\GAL4^Gα49B.PS in rdgA³ homozygotes significantly rescues the electroantennogram responses of the homozygotes.

(Kain et al., 2008)

The rate of retinal degeneration seen in rdgA³ flies is enhanced if they are also expressing one of laza^{Scer\UAS.T:Avic\GFP}, wun^Scer\UAS.cZa or wun2^Scer\UAS.cHa under the control of Scer\GAL4^ninaE.PT.

The rate of photoreceptor degeneration seen in rdgA³ flies is not slowed if they also carry wun^k12382/wun^k16806 or wun2^EP2650ex34.

(Garcia-Murillas et al., 2006)

When CG11426^{Scer\UAS.T:Avic\GFP} is expressed under the control of Scer\GAL4^ninaE.PT in rdgA³ flies, obvious degeneration is seen in the rhabdomeres of newly eclosed flies.

When CG11426^{Scer\UAS.T:Avic\GFP} is expressed under the control of Scer\GAL4^ninaE.PT in rdgA³ norpA³⁶ flies, the rhabdomeres are normal and do not show degeneration over time.

rdgA³ ; laza²² double mutant adults show photoreceptor degeneration in dim light, with the rate of degeneration being similar to that seen in rdgA³ single mutants. When flies are raised in bright light, the rate of photoreceptor degeneration in rdgA³ ; laza²²/laza²² double mutants is greatly reduced compared to that seen in rdgA³ single mutants. The rate of photoreceptor degeneration in rdgA³ ; laza²²/+ flies in bright light is intermediate between that of rdgA³ ; laza²²/laza²² and rdgA³ flies.

The decreased rate of deactivation of the light response that is seen in the electroretinogram response of rdgA³ flies following a 1 second stimulus of light is substantially rescued by laza²².

The deactivation defect seen in the electroretinogram response of rdgA³/+ flies following a 1 second stimulus of light is enhanced by expression of laza^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^ninaE.PT.

rdgA³ ; CdsA¹ double mutant flies show a higher rate of photoreceptor degeneration when raised in bright light than either rdgA³ or CdsA¹ single mutants.

sktl^Δ1-1/sktl^Δ20 enhances the rate of photoreceptor degeneration in rdgA³ mutants.

(Garcia-Murillas et al., 2006)

The rate of retinal degeneration in rdgA³ ; Gα49B¹ double mutants is slower than that of rdgA³ single mutants.

norpA³⁶ completely suppresses the retinal degeneration seen in rdgA³ flies.

(Georgiev et al., 2005)

The small amplitude of quantum bumps in Gα49B¹ photoreceptors (seen when ATP is present in the intracellular solution during recordings) can be restored to near a wild-type level if the flies are also mutant for rdgA³.

(Hardie et al., 2002)

The photoreceptor degeneration phenotype is not alleviated by the inaC⁵ mutation in double mutant flies.

(Masai et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

27903

y¹ w^* rdgA³

Notes on Origin

Discoverer

Comments

Alleles can be ranked with respect to how much R7 and R8 are affected in each mutant; rdgA¹ = rdgA² > rdgA⁴ > rdgA³.

(Harris and Stark, 1977)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

rdgA³

(Georgiev et al., 2012, Kain et al., 2009, Kain et al., 2008, Sanxaridis et al., 2007, Garcia-Murillas et al., 2006, Garcia-Murillas et al., 2006, Georgiev et al., 2005, LaLonde et al., 2005)

rdgA^PC47

(LaLonde et al., 2005, Masai et al., 1997, Yoon et al., 1996, Suzuki and Hirosawa, 1994, Stark et al., 1989, Johnson et al., 1982, Harris and Stark, 1977)

Name Synonyms

Secondary FlyBase IDs

References (19)

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