FB2024_03 , released June 25, 2024
Allele: Dmel\GαqRNAi.UAS.1f1
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General Information
Symbol
Dmel\GαqRNAi.UAS.1f1
Species
D. melanogaster
Name
FlyBase ID
FBal0219376
Feature type
allele
Associated gene
Dmel\Gαq
Associated Insertion(s)
Carried in Construct
P{UAS-Gαq.RNAi.1f1}
Also Known As
UASGq1F1
Key Links
Transgenic product class
RNAi_reagent
(Banerjee et al., 2006)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Banerjee et al., 2006)
Mutagen
in vitro construct
(Banerjee et al., 2006)
Progenitor genotype
Carried in construct
P{UAS-Gαq.RNAi.1f1}
Cytology
Description

UASt sequences govern expression an inverted repeat of 770bp of Gαq cDNA sequence from a region common to all Gαq splice variants. This sequence was amplified from of the Gαq3α isoform cDNA using PCR primers: (5'-GGTACC(538)TCACGATACTAGCAGCATCCC-3') (5'-GGATCC(1314)CGGTGTAAGCGAGCGAAG-3') for the forward sequence and (5'-TCTAGA(539)GTGCTATGATCGTCGTAGGGA-3') (5'-GGATCC(1308)TTCGCTCGCTTCTCAATTCT-3') for the inverted sequence. A 12 nucleotide linker separates the forward and reverse sequences.

(Banerjee et al., 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Gαq
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of GαqdsRNA.Scer\UAS.1f1 under the control of Scer\GAL4unspecified results in an aberrant innervation pattern of type III axons in body-wall muscle 13 in third instar larvae.

      (Park et al., 2017)

      GαqdsRNA.UAS.1f1 under the control of Scer\GAL4Gr21a.PC induces decreased avoidance to CO2, as compared to heterozygous and wild-type controls. Expression throughout development and adulthood under the control of Scer\GAL4Gr21a.PC, or only during adulthood under the control of Scer\GAL4Gr63a.PF (using Gal80[ts] for temporal control of expression), induces decreased electrophysiological responses of the basiconica rich region of the 3rd antennal segment to CO2 exposure, as compared to controls.

      (Badsha et al., 2012)

      Expression of Gα49BdsRNA.Scer\UAS.1f1 under the control of Scer\GAL4Orco.T:Hsim\VP16 does not reduce the odour response of the ab1A or ab2A olfactory receptor neurons (tested with ethyl acetate) or of the ab3A ORN (tested with methyl butyrate).

      Expression of Gα49BdsRNA.Scer\UAS.1f1 under the control of Scer\GAL4Gr21a.9.323 results in a significant decrease in the response of the ab1C ORN to CO[[2]], across a wide response range.

      Expression of Gα49BdsRNA.Scer\UAS.1f1 under the control of Scer\GAL4Gr21a.9.323 selectively in adulthood (using the temperature sensitive Scer\GAL80ts.αTub84B allele to block expression at earlier stages of development) is sufficient to decrease the response to CO[[2]] compared to wild type, at all concentrations of CO[[2]] tested.

      (Yao and Carlson, 2010)

      The electroantennograms of animals expressing Gα49BdsRNA.Scer\UAS.1f1 under the control of either Scer\GAL4Gα49B.PS or Scer\GAL4Or83b.2.642.T:Hsim\VP22 have a reduced amplitude compared to controls in response to a number of odours (ethyl acetate, butanol, propionic acid, benzaldehyde and iso-amyl acetate).

      The response to ethyl acetate of ab2 large basiconic hairs in animals expressing Gα49BdsRNA.Scer\UAS.1f1 under the control of Scer\GAL4Gα49B.PS is significantly different from that of control cells; the firing frequency of the mutant ab2a neuron in response to ethyl acetate is strongly reduced compared to controls.

      (Kain et al., 2008)

      Spontaneous odor identity discrimination is significantly disrupted in transgenic Scer\GAL4c309/+; Galpha49BdsRNA.Scer\UAS.1f1, Scer\GAL80ts.αTub84B/+ flies at the restrictive (30[o]C) but not the permissive temperature.

      (Xia and Tully, 2007)

      60% of Gα49BdsRNA.Scer\UAS.1f1; Scer\GAL4Ddc.PL flies are flightless. However, the wing posture of these flies and the spontaneous firing rate in dorsal longitudinal flight muscle is normal.

      (Banerjee et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The electroantennograms of animals co-expressing Plc21CGD11359 and Gα49BdsRNA.Scer\UAS.1f1 under the control of Scer\GAL4Or83b.2.642.T:Hsim\VP22 show a significant decrease in amplitude compared to controls in response to a number of odours (ethyl acetate, butanol, propionic acid, benzaldehyde and iso-amyl acetate).

      Expression of Gα49BdsRNA.Scer\UAS.1f1 under the control of Scer\GAL4Gα49B.PS in rdgA1 or rdgA3 homozygotes significantly rescues the electroantennogram responses of the homozygotes.

      Co-expression of Gα49BdsRNA.Scer\UAS.1f1 reduces the decrease in the amplitude of the electroantennogram in response to a number of odours (ethyl acetate, butanol, propionic acid, benzaldehyde and iso-amyl acetate) that is seen in animals expressing rdgAGD1518 under the control of Scer\GAL4Or83b.2.642.T:Hsim\VP22.

      (Kain et al., 2008)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      GαqRNAi.UAS.1f1 is rescued by Scer\GAL4Gr21a.9.323/GαqUAS.cRa

      (Yao and Carlson, 2010)
      Comments

      Co-expression of Gα49BScer\UAS.cRa restores a normal response of the ab1C olfactory receptor neuron to CO[[2]] in animals expressing Gα49BdsRNA.Scer\UAS.1f1 under the control of Scer\GAL4Gr21a.9.323.

      (Yao and Carlson, 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Gα49BdsRNA.Scer\UAS.1f1
      GαqRNAi.UAS.1f1
      GαqdsRNA.Scer\UAS.1f1
      GαqdsRNA.UAS.1f1
      Name Synonyms
      Secondary FlyBase IDs
        References (10)