FlyBase Allele Report: Dmel\spi[3]

General Information

Symbol

Dmel\spi³

Species

D. melanogaster

Name

FlyBase ID

FBal0016007

Feature type

allele

Associated gene

Dmel\spi

Associated Insertion(s)

Carried in Construct

Also Known As

spi^OE92

Key Links

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Rutledge et al., 1992, Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Eggs derived from egg chambers containing complete homozygous follicle cell clones appear normal and have two dorsal appendages.

(Lachance et al., 2009)

spi³ mutant embryos have only 1 cap attachment cell per lch5 chordotonal organ rather than the 2 per organ seen in wild-type while lch5 ligament attachment cells are often absent or deformed.

(Inbal et al., 2004)

Homozygous embryos show loss of VA2 muscle precursor cells (only 1.1% of hemisegments contain VA2 precursor cells).

(Johnson Hamlet and Perkins, 2001)

Germline clones fail to rescue the female sterile phenotype of Fs(2)Ugr.

(Sapir et al., 1998)

The DA1 muscle precursors form in all segments in spi³ heterozygous embryos.

(Yarnitzky et al., 1998)

Mutant embryos exhibit tracheal phenotype.

(Wappner et al., 1997)

Epidermal defects and fusion of the anterior and posterior commissures. In the PNS some sensory organs are missing, there is a ventral-dorsal gradient of severity. Several muscle fibres are always missing in the dorsolateral region and there are variable abnormalities in the number, shape and attachment sites of the eight ventral oblique muscle fibres.

(Rutledge et al., 1992)

embryonic lethal

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

spi³ has muscle cell of ventral acute muscle 2 | precursor phenotype, suppressible by Scer\GAL4^twi.PG/csw^{UAS.Tag:Myr(Src64B)}

(Johnson Hamlet and Perkins, 2001)

Enhancer of

Statement

Reference

spi³ is an enhancer of muscle cell of abdominal 2 dorsal acute muscle 1 | precursor phenotype of vn¹⁰⁵⁶⁷

(Yarnitzky et al., 1998)

spi³ is an enhancer of muscle cell of abdominal 3 dorsal acute muscle 1 | precursor phenotype of vn¹⁰⁵⁶⁷

(Yarnitzky et al., 1998)

spi³ is an enhancer of muscle cell of abdominal 4 dorsal acute muscle 1 | precursor phenotype of vn¹⁰⁵⁶⁷

(Yarnitzky et al., 1998)

spi³ is an enhancer of muscle cell of abdominal 5 dorsal acute muscle 1 | precursor phenotype of vn¹⁰⁵⁶⁷

(Yarnitzky et al., 1998)

spi³ is an enhancer of muscle cell of abdominal 6 dorsal acute muscle 1 | precursor phenotype of vn¹⁰⁵⁶⁷

(Yarnitzky et al., 1998)

spi³ is an enhancer of muscle cell of abdominal 7 dorsal acute muscle 1 | precursor phenotype of vn¹⁰⁵⁶⁷

(Yarnitzky et al., 1998)

Suppressor of

Statement

Reference

spi³ is a suppressor of phenotype of rho^hs.sev

(Freeman, 1994)

NOT Suppressor of

Statement

Reference

spi³ is a non-suppressor of somatic muscle cell | ectopic | embryonic stage phenotype of Scer\GAL4^69B, vn^UAS.cYa

(Yarnitzky et al., 1998)

Other

Statement

Reference

da², spi³/spi[+] has follicle cell phenotype

(Smith et al., 2002)

da², spi³ has follicle cell phenotype

(Smith et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of csw::Src64B^{src90.Scer\UAS} under the control of Scer\GAL4^twi.PG significantly suppresses the loss of VA2 muscle precursor cells seen in spi³ embryos (67% of hemisegments have VA2 cells).

(Johnson Hamlet and Perkins, 2001)

The loss of the DA1 muscle precursor seen in vn¹⁰⁵⁶⁷ embryos is dominantly enhanced by spi³. Does not suppress the production of extra eve-positive muscle precursor clusters seen in embryos expressing vn^Scer\UAS.cYa under the control of Scer\GAL4^69B.

(Yarnitzky et al., 1998)

Reduction in dose of spi rescues the rho^hs.sev eye to a near wild-type phenotype; regular array of ommatidia, regular arrangement of photoreceptors and unbroken lattice of pigment cells. Rescue is not always complete.

(Freeman, 1994)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

spi^SCP2/spi³ is rescued by Scer\GAL4^GMR.PF/spi^UAS.cSa/Scer\GAL4^hs.PB

(Huang et al., 1998)

spi^SCP2/spi³ is rescued by Scer\GAL4^αTub84B.PP/spi^UAS.cSa/Scer\GAL4^hs.PB

(Huang et al., 1998)

Partially rescued by

spi³ is partially rescued by spi^UAS.cSa/Scer\GAL4^elav.PLu

(Bergmann et al., 2002)

spi¹/spi³ is partially rescued by spi^hs.PG

(Guichard et al., 1999)

spi^SCP2/spi³ is partially rescued by spi^UAS.cSa/Scer\GAL4^hs.PB

(Huang et al., 1998)

Comments

Loss of midline glial cells in spi³ homozygous embryos is rescued by spi^Scer\UAS.cSa driven with Scer\GAL4^elav.PLu, but not with Scer\GAL4^sim.PS.

(Bergmann et al., 2002)

Animals heterozygous for spi^SCP2 and spi³ which have been rescued by spi^Scer\UAS.cSa with Scer\GAL4^hs.PB to the third instar stage show relatively normal ommatidial development at the posterior of the eye imaginal disc. More anterior columns of ommatidia have reduced photoreceptors. In the lamina, retinal projections appeared normal but LPCs show no neuronal differentiation. This phenotype is rescued by the introduction of Scer\GAL4^GMR.PF or in clones induced to produce Scer\GAL4^αTub84B.PP.

(Huang et al., 1998)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Gay, Contamine.

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

l(2)37Fa¹

l(2)OE92

spi^37Fa-1

spi³

(Lachance et al., 2009)

spi^I-IA

(Raz and Shilo, 1993)

spi^IIIA

(Tearle and Nusslein-Volhard, 1987)

spi^OE92

(Inbal et al., 2004, Bergmann et al., 2002, Reich and Shilo, 2002, Smith et al., 2002, Johnson Hamlet and Perkins, 2001, Golembo et al., 1999, Huang et al., 1998, Sapir et al., 1998, Yarnitzky et al., 1998, Gabay et al., 1997, Wappner et al., 1997, Golembo et al., 1996, Schweitzer et al., 1995, Schweitzer et al., 1995, Freeman, 1994, Rutledge et al., 1992)

Name Synonyms

Secondary FlyBase IDs

FBal0016008

References (21)

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