FlyBase Allele Report: Dmel\spi[UAS.cSa]

General Information

Symbol

Dmel\spi^UAS.cSa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Schweitzer

FlyBase ID

FBal0046513

Feature type

allele

Associated gene

Dmel\spi

Associated Insertion(s)

Carried in Construct

P{UAS-spi.m}

Also Known As

UAS-mSPI, UAS-spi

Key Links

Transgenic product class

cDNA

(Schweitzer et al., 1995)

wild_type

(Schweitzer et al., 1995)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Schweitzer et al., 1995)

wild_type

(Schweitzer et al., 1995)

Mutagen

in vitro construct

(Schweitzer et al., 1995)

Progenitor genotype

Carried in construct

P{UAS-spi.m}

Cytology

Description

Expression of a full length spi cDNA is governed by UASt regulatory sequences.

(Schweitzer et al., 1995)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

spi

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^Kr.PM

(Schweitzer et al., 1995)

Phenotype Manifest In

Detailed Description

Statement

Reference

When spi^Scer\UAS.cSa is driven by Scer\GAL4^sca-537.4 or Scer\GAL4^Dll-md23 no detectable effect on bracts is seen.

(Held, 2002)

spi^Scer\UAS.cSa, when driven by Scer\GAL4^GMR.PF produces eyes that are indistinguishable from wild-type.

(Hsiung et al., 2001)

Expression of spi^Scer\UAS.cSa under the control of Scer\GAL4^ey.PB does not result in homeotic transformation of the eye to antenna.

(Kumar and Moses, 2001)

Expression of spi^Scer\UAS.cSa using Scer\GAL4^ap-md544 does not produce a wing phenotype.

(Pickup and Banerjee, 1999)

Causes partially penetrant embryonic lethality when driven by Scer\GAL4^Kr.PM, though the cuticular morphology of the embryos is unaffected. When driven by Scer\GAL4^69B, spi^Scer\UAS.cSa can rescue the ventral Fas3 expression defect of spi³.

(Schweitzer et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

spi^UAS.cSa, Scer\GAL4^GMR.PF is an enhancer of visible phenotype of S^UAS.cGa, Scer\GAL4^GMR.PF

(Hsiung et al., 2001)

Other

Statement

Reference

S^UAS.cGa, Scer\GAL4^ap-md544, spi^UAS.cSa has visible phenotype

(Pickup and Banerjee, 1999)

S^UAS.cGa, Scer\GAL4^ap-md544, spi^UAS.cSa has increased occurrence of cell division | larval stage phenotype

(Pickup and Banerjee, 1999)

Phenotype Manifest In

Enhancer of

Statement

Reference

spi^UAS.cSa/Scer\GAL4^Bx-MS1096 is an enhancer of wing vein | ectopic phenotype of Pstu\aarA^UAS.cGa

(Gallio et al., 2002)

spi^UAS.cSa/Scer\GAL4^Bx-MS1096 is an enhancer of wing phenotype of Pstu\aarA^UAS.cGa

(Gallio et al., 2002)

spi^UAS.cSa, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of S^UAS.cGa, Scer\GAL4^GMR.PF

(Hsiung et al., 2001)

NOT Suppressor of

Statement

Reference

Scer\GAL4^tin.CΔ4/spi^UAS.cSa is a non-suppressor of adult heart chamber phenotype of ru¹

(Yu et al., 2010)

Other

Statement

Reference

Pstu\aarA^UAS.cGa, Scer\GAL4^GMR.PF, spi^UAS.cSa has eye phenotype

(Gallio et al., 2002)

S^UAS.cGa, Scer\GAL4^ap-md544, spi^UAS.cSa has wing phenotype

(Pickup and Banerjee, 1999)

S^UAS.cGa, Scer\GAL4^ap-md544, spi^UAS.cSa has wing vein phenotype

(Pickup and Banerjee, 1999)

S^UAS.cGa, Scer\GAL4^ap-md544, spi^UAS.cSa has wing disc phenotype

(Pickup and Banerjee, 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Cardiac-specific expression of spi^Scer\UAS.cSa driven by Scer\GAL4^tin.CΔ4 fails to suppress the abnormal cardiac phenotype observed in Df(3L)ED4238/+ mutants.

(Yu et al., 2010)

When Scer\GAL4^GMR.PF drives expression of both S^Scer\UAS.cGa and spi^Scer\UAS.cSa, the eye phenotype of S^Scer\UAS.cGa is strongly enhanced leading to smooth eyes with little or no red pugment and few mechanosensory bristles.

(Hsiung et al., 2001)

Coexpression of both S^Scer\UAS.cGa and spi^Scer\UAS.cSa using Scer\GAL4^ap-md544 results in ectopic proliferation of cells in the wing pouch of the wing disc and gives rise to wings which have blisters due to misapposition of the dorsal and ventral surfaces and which have missing and extra vein material.

(Pickup and Banerjee, 1999)

Xenogenetic Interactions

Statement

Reference

The wings of Pstu\aarA^Scer\UAS.cGa; Scer\GAL4^Bx-MS1096; spi^Scer\UAS.cSa flies are very small, blistered, and covered in vein-like tissue.

(Gallio et al., 2002)

Complementation and Rescue Data

Rescues

Scer\GAL4^da.G32/spi^UAS.cSa rescues spi¹/spi^SC1

(Miura et al., 2006)

Scer\GAL4^GMR.PF, spi^UAS.cSa, Scer\GAL4^hs.PB rescues spi¹/spi^SCP2

(Huang et al., 1998)

Scer\GAL4^αTub84B.PP, spi^UAS.cSa, Scer\GAL4^hs.PB rescues spi¹/spi^SCP2

(Huang et al., 1998)

Scer\GAL4^GMR.PF, spi^UAS.cSa, Scer\GAL4^hs.PB rescues spi^SCP2/spi²

(Huang et al., 1998)

Scer\GAL4^αTub84B.PP, spi^UAS.cSa, Scer\GAL4^hs.PB rescues spi^SCP2/spi²

(Huang et al., 1998)

Scer\GAL4^GMR.PF, spi^UAS.cSa, Scer\GAL4^hs.PB rescues spi^SCP2/spi³

(Huang et al., 1998)

Scer\GAL4^αTub84B.PP, spi^UAS.cSa, Scer\GAL4^hs.PB rescues spi^SCP2/spi³

(Huang et al., 1998)

Partially rescues

spi^UAS.cSa/Scer\GAL4^elav.PLu partially rescues spi³

(Bergmann et al., 2002)

spi^UAS.cSa/Scer\GAL4^hs.PB partially rescues spi¹/spi^SCP2

(Huang et al., 1998)

spi^UAS.cSa/Scer\GAL4^hs.PB partially rescues spi^SCP2/spi²

(Huang et al., 1998)

spi^UAS.cSa/Scer\GAL4^hs.PB partially rescues spi^SCP2/spi³

(Huang et al., 1998)

Comments

spi^Scer\UAS.cSa; Scer\GAL4^da.G32 rescues lethality due to spi¹/spi^SC1.

(Miura et al., 2006)

Loss of midline glial cells in spi³ homozygous embryos is rescued by spi^Scer\UAS.cSa driven with (in neurons) with Scer\GAL4^elav.PLu, but not (in glial cells) with Scer\GAL4^sim.PS.

(Bergmann et al., 2002)

Animals heterozygous for spi^SCP2 and spi¹, spi² or spi³ which have been rescued by spi^Scer\UAS.cSa with Scer\GAL4^hs.PB to the third instar stage show relatively normal ommatidial development at the posterior of the eye imaginal disc. More anterior columns of ommatidia have reduced photoreceptors. In the lamina, retinal projections appeared normal but LPCs show no neuronal differentiation. This phenotype is rescued by the introduction of Scer\GAL4^GMR.PF or in clones induced to produce Scer\GAL4^αTub84B.PP.

(Huang et al., 1998)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

spi^Scer\UAS.cSa

spi^UAS.cSa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Schweitzer

(Schweitzer et al., 1995)

Secondary FlyBase IDs

References (18)

Report Sections

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