FlyBase Allele Report: Dmel\spn-D[1]

General Information

Symbol

Dmel\spn-D¹

Species

D. melanogaster

Name

FlyBase ID

FBal0016039

Feature type

allele

Associated gene

Dmel\spn-D

Associated Insertion(s)

Carried in Construct

Also Known As

spn-D¹⁵⁰, spnD¹⁵⁰

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Gonzalez-Reyes et al., 1997)

loss of function allele

(Ghabrial et al., 1998)

Mutagen

ethyl methanesulfonate

(Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q229term.

(Abdu et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:27,166,869..27,166,869 [+]

Nucleotide change:

C27166869T

Amino acid change:

Q229term | spn-D-PA

Reported amino acid change:

Q229term

(Abdu et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle (with spn-D²)

(Bhagat et al., 2004)

decreased fecundity | female

semi-sterile (with spn-D²)

(Bhagat et al., 2004)

viable

(Ghabrial et al., 1998)

Phenotype Manifest In

dorsal appendage

(Klovstad et al., 2008, Ghabrial et al., 1998)

egg chorion | maternal effect

(Abdu et al., 2003)

egg chorion | maternal effect (with spn-D²)

(Abdu et al., 2003)

karyosome (with spn-D²)

(Abdu et al., 2003)

Detailed Description

Statement

Reference

spn-D¹ females lay eggs which show patterning defects, including having two dorsal appendages that are fused at the base, having a single dorsal appendage, and having no dorsal appendages, being completely ventralised.

(Klovstad et al., 2008)

spn-D¹ mutants have a similar frequency of single-strand annealing repair (SSA) compared to controls in a P{wIw.FRT} hemizygous assay to study DNA double-stranded break repair when assayed at 38^oC.

(Wei and Rong, 2007)

spn-D²/spn-D¹ larvae are not sensitive to MMS.

(McCaffrey et al., 2006)

spn-D²/spn-D¹ females exhibit low fertility, and meioses show reduced frequency of crossing over along most of chromosome 2, as compared to wild type.

(Bhagat et al., 2004)

78% of eggs derived from homozygous females have mutant eggshells, 22% have wild-type eggshells. 70% of eggs derived from spn-D¹/spn-D² females have mutant eggshells and defects in karyosome morphology are seen in the mutant egg chambers. spn-D¹ animals show the same survival rate after exposure to X rays or to 0.08% methyl methanesulfonate as control animals.

(Abdu et al., 2003)

Homozygous and hemizygous females produce eggs with a range of eggshell phenotypes; from resembling wild-type with two normal dorsal appendages, having two dorsal appendages fused at the base, having a single dorsal appendage to having little or no dorsal appendage material. Hemizygotes are viable. spn-D¹/spn-D² larvae show normal sensitivity to methyl methanesulfonate.

(Ghabrial et al., 1998)

weak maternal-effect lethal. Egg shape affected; in extreme cases dorsal appendages are lacking and eggs have little or no dorsal-ventral polarity; some eggs have one fused dorsal appendage. Low fecundity; eggs often slightly collapsed. embryos sometimes hatch.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

spn-D¹ has dorsal appendage phenotype, suppressible by Brca2^KO/Brca2^56E

(Klovstad et al., 2008)

spn-D¹ has egg chorion | maternal effect phenotype, suppressible by mei-W68^unspecified/mei-W68^unspecified

(Abdu et al., 2003)

spn-D²/spn-D¹ has egg chorion | maternal effect phenotype, suppressible | maternal effect by mei-41^unspecified

(Abdu et al., 2003)

spn-D²/spn-D¹ has egg chorion | maternal effect phenotype, suppressible | maternal effect by mei-W68^unspecified/mei-W68^unspecified

(Abdu et al., 2003)

spn-D²/spn-D¹ has karyosome phenotype, suppressible by mei-41^D3

(Abdu et al., 2003)

spn-D²/spn-D¹ has karyosome phenotype, suppressible by mei-W68^unspecified/mei-W68^unspecified

(Abdu et al., 2003)

spn-D²/spn-D¹ has karyosome phenotype, suppressible | partially by mei-41^D1

(Abdu et al., 2003)

spn-D¹ has egg chorion | maternal effect phenotype, suppressible by mei-41^unspecified

(Abdu et al., 2003)

spn-D¹ has egg chorion phenotype, suppressible by lok^p6

(Abdu et al., 2002)

spn-D¹ has oocyte nucleus phenotype, suppressible by lok^p6

(Abdu et al., 2002)

NOT suppressed by

Statement

Reference

spn-D¹ has egg chorion phenotype, non-suppressible by grp⁰⁶⁰³⁴

(Abdu et al., 2002)

spn-D¹ has dorsal appendage phenotype, non-suppressible by grp⁰⁶⁰³⁴

(Abdu et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The eggshell patterning defects of eggs laid by spn-D¹ females are strongly suppressed by Brca2^KO/Brca2^56E.

(Klovstad et al., 2008)

The eggshell defects seen in eggs derived from homozygous spn-D¹ females are almost completely suppressed if the females are also homozygous for mei-W68^unspecified; 98% of the eggshells are wild-type in eggs derived from double mutant females. The eggshell defects seen in eggs derived from spn-D¹/spn-D² females are completely suppressed if the females are also homozygous for mei-W68^unspecified. The karyosome defects seen in spn-D¹/spn-D² egg chambers are also suppressed by mei-W68^unspecified. The eggshell defects seen in eggs derived from homozygous spn-D¹ or spn-D¹/spn-D² females are suppressed if the females also carry mei-41^unspecified. The karyosome defects seen in spn-D¹/spn-D² egg chambers are only slightly suppressed by mei-41^D1 but are strongly suppressed by mei-41^D3. spn-D¹ spn-B^BU double mutant animals show the same survival rate after exposure to X rays or to 0.08% methyl methanesulfonate as control animals.

(Abdu et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

spn-D¹⁵⁰

(McCaffrey et al., 2006, Abdu et al., 2003, Findley et al., 2003, Abdu et al., 2002, Gonzalez-Reyes et al., 1997, Tearle and Nusslein-Volhard, 1987)

spn-D¹

spnD¹⁵⁰

(Klovstad et al., 2008, Bhagat et al., 2004, Ghabrial et al., 1998)

spnD¹

(Wei and Rong, 2007)

Name Synonyms

Secondary FlyBase IDs

References (10)

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