FlyBase Allele Report: Dmel\mei-41[D3]

General Information

Symbol

Dmel\mei-41^D3

Species

D. melanogaster

Name

FlyBase ID

FBal0012154

Feature type

allele

Associated gene

Dmel\mei-41

Associated Insertion(s)

Carried in Construct

Also Known As

mei41^D3

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: A384V.

(Laurencon et al., 2003)

In addition to the A384V amino acid replacement, which may cause the mutant phenotype, a number of additional amino acid changes are present compared to the U34925 mei-41 GenBank sequence (these "common" mutations are also present in other mei-41 mutant alleles, possibly due to variability present in the mutagenised population).

(Laurencon et al., 2003)

Nucleotide substitution: T3768A.

(Laurencon et al., 2003)

Nonsense mutation at codon 42, resulting in a stop six codons later.

(Sibon et al., 1999)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:16,393,759..16,393,759 [+]

Nucleotide change:

C16393759T

Reported nucleotide change:

T3768A

Amino acid change:

A647V | mei-41-PA

Reported amino acid change:

A384V

Comment:

Position of mutation on reference sequence inferred by FlyBase curator. Reported nucleotide change is not consistent with amino acid change.

(Laurencon et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

mei-41^D3 null embryos prematurely undergo mitosis 13; cycle 14 nuclei are defective, with massive chromosome bridging.

(Seller and O'Farrell, 2018)

mei-41^D3 mutants exhibit an accumulation of γHis2Av foci that persist through meiotic prophase, corresponding to unrepaired meiotic double-strand breaks. A mean of 21 γ-His2Av foci is present in spn-A¹ region 3 oocytes, which is similar to estimates for the total number of double strand breaks per nucleus.

(Joyce et al., 2011)

Homozygous females show a high frequency (73%) of region 3 cysts with two pro-oocytes (as assayed by c(3)G staining) compared to a frequency of only 9.5% in wild type.

(Joyce and McKim, 2009)

Irradiation of mei-41^D3 mutants with 4000 rad does not arrest cell division.

(Abdu et al., 2007)

Eggs laid by mei-41^D3 females have the correct number of dorsal appendages.

(Klattenhoff et al., 2007)

The S-phase index is increased 2.4-fold in mei-41^D3 double mutants compared to wild-type.

(Sakaguchi and Steward, 2007)

mei-41^D3 mutant wing discs fail to arrest in response to a range of irradiation doses.

mei-41^D3 mutant cells exhibit DNA breaks and telomere fusions in metaphase and anaphase at almost the same level as wild-type cells.

(Oikemus et al., 2006)

Mutant eyes (generated using the "EGUF" system) do not display morphological defects when larvae are raised on media containing 2 mmol caffeine/L and 3 mmol hydroxyurea/L.

(Silva et al., 2006)

Heterozygotes are sensitive to methyl methanesulfonate compared to wild-type animals. Hemizygous larvae show 98% loss of the G₂/M checkpoint (this number is the average number of mitotic cells per eye disc after exposure of male hemizygous larvae to 500R of X rays expressed as a percentage of the number of mitotic cells per eye disc before irradiation, wild-type values range from 5 to 15%).

(Laurencon et al., 2003)

No precocious anaphases are seen in mutant oocyte nuclei.

(McKim et al., 2000)

Homozygous females produce cellularised embryos at a very low frequency (2%).

(Price et al., 2000)

Defects are seen during mitosis 13 and 14 in embryos derived from homozygous females mated to wild-type males. Anastral spindles with broad poles and numerous free cytoplasmic microtubules are seen.

(Sibon et al., 2000)

Homozygous females are sterile and produce eggs that do not hatch. Most of the embryos derived from these eggs develop to the syncytial blastoderm stage and have relatively normal nuclear morphology up to division 12 or 13. Later embryos show significant variability in nuclear size and distribution. The embryos fail to cellularise after mitosis 13 and proceed through at least two additional syncytial cycles of spindle assembly and disassembly. The lengths of interphase and mitosis are close to wild type until division 11, however, during divisions 12 and 13 interphase is significantly shorter than in wild-type embryos. Embryos derived from homozygous females do not delay nuclear envelope breakdown in response to aphidicolin treatment, in contrast to wild-type embryos. Homozygotes are more sensitive to hydroxyurea and methyl methanesulfonate than wild-type. Approximately 4% of embryos derived from mei-41^D3/mei-41^D5 females hatch at 25^oC, while approximately 45% hatch at 20^oC.

(Sibon et al., 1999)

Mutant larvae die on exposure to MMS. Homozygous females are sterile.

(Banga et al., 1995)

Defect in a cell cycle checkpoint so that cells with damaged DNA (for example by X ray) are allowed to enter mitotic metaphase.

(Hari et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

mei-41^D5/mei-41^D3 has lethal | embryonic stage | maternal effect phenotype, enhanceable by grp[+]/grp^fsA4

(Sibon et al., 1999)

Suppressed by

Statement

Reference

mei-41^D5/mei-41^D3 has lethal | maternal effect | embryonic stage phenotype, suppressible by CycA^unspecified/CycB^unspecified

(Sibon et al., 1999)

mei-41^D3 has lethal | recessive | maternal effect | embryonic stage phenotype, suppressible by CycA^unspecified

(Sibon et al., 1999)

mei-41^D3 has lethal | recessive | maternal effect | embryonic stage phenotype, suppressible by CycB^unspecified

(Sibon et al., 1999)

Enhancer of

Statement

Reference

mei-41[+]/mei-41^D3 is an enhancer of decreased cell number | adult stage | female | temperature conditional phenotype of Crei\I-CreI^hs.PR

(Ma et al., 2016)

mei-41^D3/mei-41^D3 is an enhancer of decreased cell number | adult stage | female | temperature conditional phenotype of Crei\I-CreI^hs.PR

(Ma et al., 2016)

mei-41^D3 is an enhancer of abnormal mitotic cell cycle phenotype of tefu¹

(Oikemus et al., 2006)

Suppressor of

Statement

Reference

mei-41^D3 is a suppressor of abnormal mitotic cell cycle phenotype of Set8²⁰

(Sakaguchi and Steward, 2007)

mei-41^D3 is a suppressor of abnormal mitotic cell cycle phenotype of dup^a3

(Garner et al., 2001)

NOT Suppressor of

Statement

Reference

mei-41^D3 is a non-suppressor of abnormal cell polarity | germline clone phenotype of Aatf^nutmeg-21.3

(Jagut et al., 2013)

Other

Statement

Reference

Crei\I-CreI^hs.PR, mei-41^D3 has decreased cell number | female | adult stage phenotype

(Ma et al., 2016)

grp^fsA4, mei-41^D3 has chemical sensitive phenotype

(Sibon et al., 1999)

CycA^unspecified, CycB^unspecified, mei-41^D5/mei-41^D3 has lethal | maternal effect | embryonic stage phenotype

(Sibon et al., 1999)

Phenotype Manifest In

Suppressed by

Statement

Reference

mei-41^D3 has phenotype, suppressible by Wee1^+tPa

(Price et al., 2000)

Enhancer of

Statement

Reference

mei-41[+]/mei-41^D3 is an enhancer of female germline stem cell | adult stage | temperature conditional phenotype of Crei\I-CreI^hs.PR

(Ma et al., 2016)

mei-41^D3/mei-41^D3 is an enhancer of female germline stem cell | adult stage | temperature conditional phenotype of Crei\I-CreI^hs.PR

(Ma et al., 2016)

mei-41^D3 is an enhancer of FlyBase_internalproperty type:chromosome:chromosome phenotype of Set8²⁰

(Sakaguchi and Steward, 2007)

mei-41^D3 is an enhancer of condensed chromosome phenotype of Set8²⁰

(Sakaguchi and Steward, 2007)

mei-41[+]/mei-41^D3 is an enhancer of phenotype of Wee1^DS1

(Price et al., 2000)

mei-41^D3/mei-41^D3 is an enhancer of phenotype of Wee1^DS1

(Price et al., 2000)

Suppressor of

Statement

Reference

mei-41^D3 is a suppressor of karyosome | heat sensitive phenotype of tefu^atm-8

(Joyce et al., 2011)

mei-41^D3 is a suppressor | partially of dorsal appendage | maternal effect phenotype of rhi⁰²⁰⁸⁶/rhi^KG00910

(Klattenhoff et al., 2009)

mei-41^D3/mei-41^D3 is a suppressor | partially of dorsal appendage | maternal effect phenotype of armi^72.1/armi¹

(Klattenhoff et al., 2009)

mei-41^D3/mei-41^D3 is a suppressor | partially of dorsal appendage phenotype of armi^72.1/armi¹

(Klattenhoff et al., 2007)

mei-41^D3/mei-41^D3 is a suppressor of microtubule organizing center & oocyte phenotype of armi^72.1/armi¹

(Klattenhoff et al., 2007)

mei-41^D3/mei-41^D3 is a suppressor of dorsal appendage phenotype of aub^HN2/aub^QC42

(Klattenhoff et al., 2007)

mei-41^D3 is a suppressor of karyosome phenotype of spn-D²/spn-D¹

(Abdu et al., 2003)

mei-41^D3/mei-41^RT1 is a suppressor of dorsal appendage phenotype of mus301⁰⁹⁴

(Masrouha et al., 2003)

mei-41^D3/mei-41^RT1 is a suppressor of dorsal appendage phenotype of spn-B^unspecified

(Masrouha et al., 2003)

mei-41^D3 is a suppressor of mitotic cell cycle phenotype of dup^a3

(Garner et al., 2001)

mei-41^D3/mei-41^D3 is a suppressor | maternal effect of egg chorion | maternal effect phenotype of mus301^D4/mus301⁰⁹⁴

(Ghabrial and Schupbach, 1999)

mei-41[+]/mei-41^D3 is a suppressor | maternal effect | partially of egg chorion | maternal effect phenotype of mus301^D4/mus301⁰⁹⁴

(Ghabrial and Schupbach, 1999)

mei-41^D3/mei-41^RT1 is a suppressor of egg chorion | maternal effect phenotype of spn-B^BU

(Ghabrial and Schupbach, 1999)

mei-41[+]/mei-41^D3 is a suppressor of egg chorion | maternal effect phenotype of spn-B^BU

(Ghabrial and Schupbach, 1999)

NOT Suppressor of

Statement

Reference

mei-41^D3 is a non-suppressor of egg chamber | germline clone phenotype of Aatf^nutmeg-21.3

(Jagut et al., 2013)

mei-41^D3 is a non-suppressor of oocyte | germline clone phenotype of Aatf^nutmeg-21.3

(Jagut et al., 2013)

mei-41^D3 is a non-suppressor of centrosome | germline clone phenotype of Aatf^nutmeg-21.3

(Jagut et al., 2013)

mei-41^D3 is a non-suppressor of karyosome phenotype of Brca2^KO/Brca2^56E

(Klovstad et al., 2008)

mei-41^D3 is a non-suppressor of dorsal appendage phenotype of Brca2^KO/Brca2^56E

(Klovstad et al., 2008)

mei-41^D3 is a non-suppressor of dorsal appendage phenotype of spn-E¹

(Klattenhoff et al., 2007)

mei-41^D3 is a non-suppressor of karyosome phenotype of Src64B^Δ17

(Djagaeva et al., 2005)

mei-41^D3 is a non-suppressor of karyosome phenotype of enc^R17

(Van Buskirk et al., 2000)

mei-41^D3 is a non-suppressor of egg chorion phenotype of enc^R17

(Van Buskirk et al., 2000)

mei-41^D3/mei-41^D3 is a non-suppressor of egg chorion | maternal effect phenotype of vas^RG53/vas^PH165

(Ghabrial and Schupbach, 1999)

Other

Statement

Reference

Crei\I-CreI^hs.PR, mei-41^D3 has female germline stem cell | adult stage phenotype

(Ma et al., 2016)

Df(2R)LL5, mei-41^D3, mei-W68¹ has oocyte nucleus & meiotic cell cycle phenotype

(McKim et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

mei-41^D3 is unable to suppress the early oogenesis arrest and oocyte polarity phenotypes seen in Aatf^nutmeg-21.3 mutant germline clones.

(Jagut et al., 2013)

The karyosome morphology defects found in female tefu^atm-8 mutants at the restrictive temperature of 25[o]C are suppressed in a mei-41^D3 mutant background.

(Joyce et al., 2011)

33% of eggs derived from mei-41^D3 ; rhi^KG00910/rhi⁰²⁰⁸⁶ double mutant females have two normal dorsal appendages.

56% of eggs derived from mei-41^D3/mei-41^D3 ; armi¹/armi^72.1 double mutant females have normal dorsal appendages, but the fraction of embryos with normal dorsal appendages increases to 83% if the females are fed caffeine.

(Klattenhoff et al., 2009)

The addition of mei-41^D3 results in little or no suppression of the eggshell patterning and karyosome formation defects of Brca2^KO/Brca2^56E females.

Mutant larvae do not show efficient G2 arrest after exposure to 500Rad or 4000Rad irradiation. The mutant larvae do not show a reduction in BrdU incorporation after a 4000Rad irradiation exposure, in contrast to wild-type larvae.

(Klovstad et al., 2008)

The mei-41^D3 mutation partially suppresses the fused dorsal appendage phenotype of armi^72.1/armi¹ eggs; 56% of mei-41^D3; armi^72.1/armi¹ eggs show wild-type appendage morphology.

The microtubule-organizing center phenotype of armi^72.1/armi¹ oocytes is partially suppressed by the mei-41^D3 mutation.

The fused dorsal appendage phenotype of spn-E¹ eggs is not suppressed by mei-41^D3.

(Klattenhoff et al., 2007)

In mei-41^D3;pr-set7²⁰ double mutants, the mitotic index, significantly reduced in pr-set7²⁰ mutants, is rescued to be similar to that observed in wild-type and the homozygous mei-41^D3 mutant. The number of prophase cells is reduced compared with the number observed in pr-set7²⁰ homozygotes and becomes similar to wild-type and pr-set7²⁰ mutants. The ratio of anaphase/telophase cells with lagging chromatids is increased when the DNA damage checkpoint is abolished in the double homozygous mei-41^D3;pr-set7²⁰ mutant, indicating that the defect in chromomsome condensation is independent of checkpoint activation and that, in the absence of the checkpoint, the severity of the chromosome condensation defect is enhanced.

(Sakaguchi and Steward, 2007)

tefu¹ mei-41^D3 double mutant cells exhibit more DNA breaks and telomere fusions in metaphase and anaphase than in wild-type cells.

(Oikemus et al., 2006)

The karyosome defects seen in spn-D¹/spn-D² egg chambers are strongly suppressed by mei-41^D3.

(Abdu et al., 2003)

mei-41^D3/mei-41^RT1 partially suppresses the dorsal appendage defects of mus301⁰⁹⁴ homozygotes; 70% of eggs derived from double mutant females have wild-type dorsal appendages, 21% have fused dorsal appendages and 9% have no dorsal appendages. mei-41^D3/mei-41^RT1 partially suppresses the dorsal appendage defects of eggs derived from spn-B^unspecified females.

(Masrouha et al., 2003)

In mei-41^D3; mei-W68¹/Df(2R)LL5 oocytes most nuclei exhibit either disorganised spindles and multiple chromatin masses or premature anaphases.

In mei-41^D3; mei-W68¹/Df(2R)LL5 oocyte nuclei most nuclei are either exhibit disorganised spindles and multiple chromatin masses or premature anaphases.

(McKim et al., 2000)

20% of embryos derived from homozygous mei-41^D3 females cellularise if they are carrying one copy of wee^+tPa, and 50% cellularise if they are carrying two copies of wee^+tPa (and some develop to adulthood in this case). The incompletely penetrant syncytial arrest phenotype of embryos derived from homozygous wee^DS1 females is enhanced if the females also carry mei-41^D3, so that only 39% of the embryos cellularise if the females carry one copy of mei-41^D3 and only 29% of the embryos cellularise if the females carry two copies of mei-41^D3.

(Price et al., 2000)

The egg shell ventralisation and karyosome defects seen in hemizygous enc^R17 females are not suppressed by mei-41^D3.

(Van Buskirk et al., 2000)

The eggshell patterning defects of eggs derived from spn-B^BU/spn-B^BU females are suppressed if the females also carry mei-41^D3/+ or mei-41^D3/mei-41^RT1. The eggshell patterning defects of eggs derived from mus301^D4/mus301⁰⁹⁴ females are suppressed if the females also carry mei-41^D3/+ or mei-41^D3/mei-41^D3. The eggshell patterning defects of eggs derived from vas^RG53/vas^PH165 females are not suppressed if the females also carry mei-41^D3/mei-41^D3.

(Ghabrial and Schupbach, 1999)

mei-41^D3 grp^fsA4 double heterozygous larvae are more sensitive to methyl methanesulfonate than single heterozygous larvae. If mei-41^D3/mei-41^D5 females are also heterozygous for grp^fsA4, the hatch rates are reduced to 1% and 23% at 25^oC and 20^oC respectively. The lethality of embryos derived from mei-41^D3/mei-41^D5 females is partly rescued if the females are also heterozygous for either CycA^unspecified or CycB^unspecified, and greater rescue is seen if the females are also heterozygous for both CycA^unspecified and CycB^unspecified.

(Sibon et al., 1999)

Xenogenetic Interactions

Statement

Reference

The loss of germline stem cells (GSCs) observed upon heat-shock induced expression of Crei\I-CreI^hs.PR (to introduce DNA damage) is further exacerbated by mei-41^D3 in homo- or heterozygosity.

mei-41^D3;Crei\I-CreI^hs.PR double mutants have fewer GSCs per germarium than wild-type controls even without the induction of DNA damage by heat-shock.

(Ma et al., 2016)

Complementation and Rescue Data

Rescued by

mei-41^D3 is rescued by mei-41^+t10.5

(Banga et al., 1995)

Comments

Both MMS sensitivity and sterility phenotypes are rescued by mei-41^+t10.5.

(Banga et al., 1995)

Images (0)

Mutant

Wild-type

Stocks (1)

Kyoto

101012

C(1)DX, y¹ f¹ / w¹ mei-41^D3

Notes on Origin

Discoverer

Comments

The recovery of chromosomes undergoing P-element transposition in the presence of P\T^Δ2-3 is severely reduced in mei-41^D3 flies, suggesting that mei-41⁺ function is required for the recovery of these chromosomes. This instability is reversed if P\T^Sal is also present.

(Banga et al., 1991)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

mei-41^D3

(Colmenares et al., 2017, Ma et al., 2016, Blythe and Wieschaus, 2015, Iampietro et al., 2014, Jagut et al., 2013, Joyce et al., 2011, Joyce and McKim, 2009, Klapholz et al., 2009, Klovstad et al., 2008, Sakaguchi et al., 2008, Abdu et al., 2007, Klattenhoff et al., 2007, Pane et al., 2007, Sakaguchi and Steward, 2007, McCaffrey et al., 2006, Oikemus et al., 2006, Silva et al., 2006)

mei41^D3

(Seller and O'Farrell, 2018, Jagut et al., 2013, Klattenhoff et al., 2009, Chen et al., 2007, Cohen et al., 2003)

mei41D3

(Kanke et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (42)

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