FlyBase Allele Report: Dmel\mus301[094]

General Information

Symbol

Dmel\mus301⁰⁹⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0016036

Feature type

allele

Associated gene

Dmel\mus301

Associated Insertion(s)

Carried in Construct

Also Known As

spn-C⁰⁹⁴, spnC⁰⁹⁴

Key Links

Genomic Maps

JBrowse

Allele class

antimorphic allele - genetic evidence

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(McCaffrey et al., 2006)

antimorphic allele - genetic evidence

(Gonzalez-Reyes et al., 1997)

Mutagen

ethyl methanesulfonate

(Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Nucleotide substitution: G2101A.

(McCaffrey et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:8,139,937..8,139,937 [+]

Nucleotide change:

G8139937A

Reported nucleotide change:

G2101A

Amino acid change:

G637E | mus301-PA

Reported amino acid change:

G637Q

Comment:

The reported amino acid change is an apparent typographical error. Changing G to A at the reported nucleotide location results in a Gly to Glu mutation, rather than Gly to Gln. The A of the initiator ATG was used used as the +1 nucleotide.

(McCaffrey et al., 2006)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

chemical sensitive

(Abdu et al., 2003)

chemical sensitive (with Df(3L)66C-G28)

(McCaffrey et al., 2006)

chemical sensitive (with mus301⁶⁶⁰)

(McCaffrey et al., 2006)

chemical sensitive (with mus301^D1)

(McCaffrey et al., 2006)

chemical sensitive (with mus301^D2)

(McCaffrey et al., 2006)

chemical sensitive (with mus301^D4)

(McCaffrey et al., 2006)

decreased fecundity | female

Phenotype Manifest In

dorsal appendage

(Masrouha et al., 2003)

egg | dorsal

(Gonzalez-Reyes et al., 1997)

egg | maternal effect (with Df(3L)66C-G28)

(McCaffrey et al., 2006)

egg | maternal effect (with mus301^D1)

(McCaffrey et al., 2006)

egg chamber

(Gonzalez-Reyes and St. Johnston, 1994)

egg chorion | maternal effect (with mus301^D4)

(Ghabrial and Schupbach, 1999)

germline cyst

(Huynh and St. Johnston, 2000)

karyosome

(Gonzalez-Reyes et al., 1997)

karyosome (with Df(3L)66C-G28)

(McCaffrey et al., 2006)

karyosome (with mus301^D1)

(McCaffrey et al., 2006)

nurse cell

(Gonzalez-Reyes et al., 1997)

oocyte

(Gonzalez-Reyes and St. Johnston, 1998, Gonzalez-Reyes et al., 1997)

oocyte nucleus (with Df(3L)66C-G28)

(McCaffrey et al., 2006)

oocyte nucleus (with mus301^D1)

(McCaffrey et al., 2006)

synaptonemal complex

(Huynh and St. Johnston, 2000)

Detailed Description

Statement

Reference

100% of mus301⁰⁹⁴/Df(3L)66C-G28 egg chambers have a mutant karyosome and 2% have misplaced oocytes. 49% of eggs laid by mus301⁰⁹⁴/Df(3L)66C-G28 females are ventralised.

100% of mus301⁰⁹⁴/mus301^D1 egg chambers have a mutant karyosome and 2% have misplaced oocytes. 21% of eggs laid by mus301⁰⁹⁴/mus301^D1 females are ventralised.

mus301⁰⁹⁴/Df(3L)66C-G28 females show 17% X chromosome nondisjunction. mus301⁰⁹⁴/Df(3L)ZP3 females show 15% X chromosome nondisjunction.

mus301⁰⁹⁴/Df(3L)66C-G28, mus301⁰⁹⁴/mus301⁶⁶⁰, mus301⁰⁹⁴/mus301^D1, mus301⁰⁹⁴/mus301^D2 and mus301⁰⁹⁴/mus301^D4 larvae are sensitive to MMS compared to controls.

(McCaffrey et al., 2006)

mus301⁰⁹⁴ animals do not survive exposure to 0.08% methyl methanesulfonate.

(Abdu et al., 2003)

26% of eggs laid by homozygous females have wild-type dorsal appendages, 50% have fused dorsal appendages and 24% have no dorsal appendages.

(Masrouha et al., 2003)

In contrast to wild-type ovaries, where the synaptonemal complex (SC) is always restricted to the oocyte by region 2b, mus301⁰⁹⁴ mutant females show a significant delay in the process, with cysts with more than 1 cell in synapsis in region 3 of the germarium and in stage 1 and stage 2 of the vitellarium. Cysts with 4 cells in meiosis are often found in region 2b of the germarium.

(Huynh and St. Johnston, 2000)

The oocyte is not correctly positioned in 32% of cysts in homozygous females.

(Gonzalez-Reyes and St. Johnston, 1998)

Eggs exhibit a strong ventralised phenotype: eggs are longer than wild type and are completely symmetric along the DV axis. Egg chambers of females exhibit a partially penetrant disruption in the positioning of the oocyte which can be located anywhere in the egg chamber. Almost all oocytes lack a karyosome and the oocyte chromosomes are arranged instead in thread-like figures with irregular shape. Germline clones in egg chambers are composed of wild type follicle cells, mutant nurse cells, misplaced oocyte, oocytes that give rise to ventralised eggs and oocytes that lack a karyosome.

(Gonzalez-Reyes et al., 1997)

In approximately 60% of mutant egg chambers, the oocyte fails to move to the posterior and lies either at the anterior of the germline cyst or in the middle, with nurse cells on both sides. Follicle cells migrate to the middle of the bipolar egg chamber, to form a belt around the oocyte. Germline clones show the same mutant phenotype.

(Gonzalez-Reyes and St. Johnston, 1994)

Posterior localization of vas protein.

(Lasko and Ashburner, 1990)

maternal-effect lethal Egg shape affected; in extreme cases dorsal appendages are lacking and eggs have little or no dorsal-ventral polarity; some eggs have one fused dorsal appendage. Low fecundity; eggs often slightly collapsed.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

mus301⁰⁹⁴ has dorsal appendage phenotype, suppressible by mei-41^D3/mei-41^RT1

(Masrouha et al., 2003)

mus301^D4/mus301⁰⁹⁴ has egg chorion | maternal effect phenotype, suppressible | maternal effect by mei-W68^unspecified/Df(2R)LL5

(Ghabrial and Schupbach, 1999)

mus301^D4/mus301⁰⁹⁴ has egg chorion | maternal effect phenotype, suppressible | maternal effect by mei-41^D3/mei-41^D3

(Ghabrial and Schupbach, 1999)

mus301^D4/mus301⁰⁹⁴ has egg chorion | maternal effect phenotype, suppressible | maternal effect | partially by mei-41[+]/mei-41^D3

(Ghabrial and Schupbach, 1999)

NOT suppressed by

Statement

Reference

mus301⁰⁹⁴ has dorsal appendage phenotype, non-suppressible by barr^+tMa/vls^+tMa/lok^5'.tMa/Df(2L)pr2b/Df(2L)be408/lok^3'.tMa

(Masrouha et al., 2003)

Other

Statement

Reference

mus301⁰⁹⁴, spn-E¹ has oocyte phenotype

(Gonzalez-Reyes et al., 1997)

mus301⁰⁹⁴, spn-A³ has oocyte phenotype

(Gonzalez-Reyes et al., 1997)

mus301⁰⁹⁴, spn-B¹ has oocyte phenotype

(Gonzalez-Reyes et al., 1997)

Additional Comments

Genetic Interactions

Statement

Reference

mei-41^D3/mei-41^RT1 partially suppresses the dorsal appendage defects of mus301⁰⁹⁴ homozygotes; 70% of eggs derived from double mutant females have wild-type dorsal appendages, 21% have fused dorsal appendages and 9% have no dorsal appendages. The dorsal appendage defects of eggs derived from mus301⁰⁹⁴ females are not suppressed by Df(2L)be408 P{CG10728^+tMa}/Df(2L)pr2b P{barr^+tMa}.

(Masrouha et al., 2003)

The eggshell patterning defects of eggs derived from mus301^D4/mus301⁰⁹⁴ females are suppressed if the females also carry mei-W68^unspecified/Df(2R)LL5, mei-41^D3/+ or mei-41^D3/mei-41^D3.

(Ghabrial and Schupbach, 1999)

Double mutant stage 5-6 egg chambers with spn-A³, spn-B¹ or spn-E¹ exhibit a two-oocyte phenotype: the second pro-oocyte develops as an oocyte rather than a nurse cell. The double mutants delay but do not block the decision between the two pro-oocytes as one of the two cells always becomes a nurse cell eventually.

(Gonzalez-Reyes et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

mus301^D4

(Ghabrial and Schupbach, 1999)

mus301^D2

(Ghabrial and Schupbach, 1999)

mus301^D1

(Ghabrial and Schupbach, 1999)

Rescued by

mus301⁰⁹⁴ is rescued by mus301^+t6.2

(McCaffrey et al., 2006)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

mus301⁰⁹⁴

(McCaffrey et al., 2006)

spn-C⁰⁹⁴

(Abdu et al., 2003, Ghabrial and Schupbach, 1999, Gonzalez-Reyes and St. Johnston, 1998, Gonzalez-Reyes et al., 1997, Gonzalez-Reyes and St. Johnston, 1994, Lasko and Ashburner, 1990, Tearle and Nusslein-Volhard, 1987)

spn-C¹

spnC⁰⁹⁴

(Masrouha et al., 2003, Huynh and St. Johnston, 2000)

Name Synonyms

Secondary FlyBase IDs

References (10)

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