FlyBase Allele Report: Dmel\grp[fsA4]

General Information

Symbol

Dmel\grp^fsA4

Species

D. melanogaster

Name

FlyBase ID

FBal0062815

Feature type

allele

Associated gene

Dmel\grp

Associated Insertion(s)

P{hsneo}grp^fsA4

Carried in Construct

Also Known As

grp^fs(A)4

Key Links

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Sibon et al., 1997)

Mutagen

P-element activity

(Sibon et al., 1997)

Progenitor genotype

Associated Insertion(s)

P{hsneo}grp^fsA4

Cytology

Description

P{hsneo} insertion is mapped to within 300bp of the P-element of grp⁰⁶⁰³⁴.

(Sibon et al., 1997)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal DNA repair

(Brodsky et al., 2000)

abnormal mitotic cell cycle

(Großhans et al., 2003)

abnormal mitotic cell cycle | embryonic stage

(Aydogan et al., 2018)

chemical sensitive

(Sakaguchi et al., 2008)

chemical sensitive | recessive

(Masrouha et al., 2003, Sibon et al., 1999)

lethal - all die during embryonic stage | maternal effect

(Sibon et al., 1997)

some die during embryonic stage | maternal effect

(Sibon et al., 1997)

Phenotype Manifest In

centrosome | embryonic stage

(Takada et al., 2003)

embryo | embryonic stage 4

(Sibon et al., 1999)

spindle | embryonic stage

(Takada et al., 2003)

Detailed Description

Statement

Reference

grp^fsA4/+ embryos exhibit a significant decrease in S-phase length while they do not exhibit any changes in centriole growth rate, growth period or size when compared to controls.

(Aydogan et al., 2018)

Homozygotes show strongly reduced survival after exposure to hydroxyurea or methylmethanesulfonate.

(Sakaguchi et al., 2008)

0% of egg chambers have a mutant karyosome in mutant females.

(McCaffrey et al., 2006)

In embryos from grp^fsA4 mothers, the chromosomes arrest at cycle 13 and lower, while chromosomes arrest at cycle 14 in wild-type embryos.

(Großhans et al., 2003)

Homozygous larvae are sensitive to methyl methanesulfonate and hydroxyurea compared to control larvae.

(Masrouha et al., 2003)

Mutant embryos exhibit spindle assembly and centrosome defects beginning at mitosis 12, with severe defects consistently observed by mitosis 13.

(Takada et al., 2003)

In the absence of irradiation, grp^fsA4 animals have a normal external appearance, normal imaginal disc morphology and normal numbers of mitotic cells in the discs. After irradiation, the number of mitotic cells in grp^fsA4 discs is greater than the number found in irradiated wild-type discs.

(Brodsky et al., 2000)

Embryos derived from homozygous females show defects in syncytial-division timing. Homozygotes are more sensitive to hydroxyurea and methyl methanesulfonate than wild-type.

(Sibon et al., 1999)

Mutant embryos fail to undergo changes in cell-cycle timing and embryo morphology associated with the midblastula transition (MBT). Interphase length does not increase significantly during divisions 11-13, mitosis 13 is immediately followed by at least two additional syncytial cycles of spindle assembly/disassembly and nuclear envelope breakdown/assembly.

(Sibon et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

grp^fsA4 has abnormal mitotic cell cycle phenotype, suppressible by ms(3)K81^unspecified

(Großhans et al., 2003)

Enhancer of

Statement

Reference

grp[+]/grp^fsA4 is an enhancer of lethal | embryonic stage | maternal effect phenotype of mei-41^D5/mei-41^D3

(Sibon et al., 1999)

Other

Statement

Reference

grp^fsA4, mei-41^D3 has chemical sensitive phenotype

(Sibon et al., 1999)

Phenotype Manifest In

Suppressed by

Statement

Reference

grp^fsA4 has centrosome | embryonic stage phenotype, suppressible by lok^p6

(Takada et al., 2003)

grp^fsA4 has spindle | embryonic stage phenotype, suppressible by lok^p6

(Takada et al., 2003)

NOT Suppressor of

Statement

Reference

grp^fsA4 is a non-suppressor of karyosome phenotype of Df(3L)66C-G28/mus301⁶⁶⁰

(McCaffrey et al., 2006)

grp^fsA4/grp^fsA4 is a non-suppressor of dorsal appendage phenotype of spn-B^unspecified

(Masrouha et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

In haploid grp^fsA4 embryos, created by fertilization of eggs by ms(3)K81^unspecified fathers, chromosomes arrest at cycle 14, as in wild-type embryos. This is in contrast to the premature arrest seen in diploid grp^fsA4 embryos.

(Großhans et al., 2003)

The dorsal appendage defects of eggs derived from spn-B^unspecified females are not suppressed by grp^fsA4/grp^fsA4.

(Masrouha et al., 2003)

In grp^fsA4, lok^p6 embryos, essentially normal centrosomes and spindles are seen through mitosis 13.

(Takada et al., 2003)

Approximately 4% of embryos derived from mei-41^D3/mei-41^D5 females hatch at 25^oC, while approximately 45% hatch at 20^oC. If the females are also heterozygous for grp^fsA4, the hatch rates are reduced to 1% and 23% at 25^oC and 20^oC respectively. mei-41^D3 grp^fsA4 double heterozygous larvae are more sensitive to methyl methanesulfonate than single heterozygous larvae.

(Sibon et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Reversion analysis recovers homozygous female fertile flies and other sterile flies.

(Sibon et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

fs(A)4

(Sibon et al., 1997)

grapes^fs(1)A4

(Großhans et al., 2003)

grp^fs(A)4

(Jaklevic et al., 2006, Takada et al., 2003, Brodsky et al., 2000, Sibon et al., 1999, Sibon et al., 1997)

grp^fs(A)

(Sibon et al., 2000)

grp^fsA4

(Aydogan et al., 2020, Aydogan et al., 2018, Takada et al., 2015, Sakaguchi et al., 2008, Sakaguchi and Steward, 2007, McCaffrey et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (14)

Report Sections

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