FlyBase Allele Report: Dmel\boss[1]

General Information

Symbol

Dmel\boss¹

Species

D. melanogaster

Name

FlyBase ID

FBal0019768

Feature type

allele

Associated gene

Dmel\boss

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

loss of function allele

Mutagen

X ray

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Fortini et al., 1992, Gaul et al., 1992)

loss of function allele

(Bonfini et al., 1992, Rogge et al., 1991)

Mutagen

X ray

(Hart et al., 1990)

Progenitor genotype

Cytology

Polytene chromosomes normal

Polytene chromosomes normal.

(Hart et al., 1990)

Description

23bp deletion within the boss coding region between nucleotides 306 and 328 causes a frameshift at amino acid 45 and results in an additional 24 amino acids.

(Hart et al., 1990)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:25,964,000..25,964,022 [+]

Comment:

23 bp deletion

(Hart et al., 1990)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal stress response

(Kohyama-Koganeya et al., 2008)

decreased body size | adult stage

(Kohyama-Koganeya et al., 2008)

decreased body size | pupal stage

(Kohyama-Koganeya et al., 2008)

viable

(Hart et al., 1990)

Phenotype Manifest In

embryonic/larval oenocyte

(Kohyama-Koganeya et al., 2008)

photoreceptor cell R7

(Sunio et al., 1999, Sevrioukov et al., 1998, Kolodkin et al., 1994, Fortini et al., 1992, Mlodzik et al., 1992, Rogge et al., 1992)

Detailed Description

Statement

Reference

Hemolymph glucose, trehalose and triacylglycerol levels are elevated in mutant larvae compared to controls.

The oenocytes of mutant larvae contain lipid droplets regardless of whether the larvae are fed a standard diet or are deprived of food (wild-type oenocytes only accumulate lipid droplets when they are starved).

Mutant adults show reduced survival under starvation conditions compared to wild-type flies; after 48 hours of starvation 62% of wild-type flies remain viable, whereas only 29% of mutant flies survive. The mutant flies show a more rapid depletion of lipid stores than control flies.

(Kohyama-Koganeya et al., 2008)

Mutant flies lack the R7 photoreceptor cells.

(Sunio et al., 1999)

The mutant phenotype of this allele can be completely rescued by Dvir\boss^+t9.

(Hart et al., 1993)

R7 precursor cell develops into an equatorial cone cell (EQC). P{HZ}klg^H214 marker expression is present in R7 precursor cell and persists in the EQC.

(Mlodzik et al., 1992)

Homozygous ommatidia lack photoreceptor cell R7. Ommatidia of double mutants with Gap1^sxt-BJ61 display extra R7 cells.

(Rogge et al., 1992)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

boss¹ has photoreceptor cell R7 phenotype, suppressible by RasGAP1^B1

(Gaul et al., 1992)

boss¹ has photoreceptor cell R7 phenotype, suppressible by RasGAP1^B2

(Gaul et al., 1992)

boss¹ has photoreceptor cell R7 phenotype, suppressible by RasGAP1^B3

(Gaul et al., 1992)

boss¹ has photoreceptor cell R7 phenotype, suppressible by RasGAP1^B4

(Gaul et al., 1992)

boss¹ has photoreceptor cell R7 phenotype, suppressible by RasGAP1^B5

(Gaul et al., 1992)

Suppressor of

Statement

Reference

boss¹ is a suppressor of phenotype of Src42A^KR.hs.2sev

(Takahashi et al., 1996)

Other

Statement

Reference

RasGAP1^B2, boss¹ has photoreceptor cell R7 | ectopic phenotype

(Gaul et al., 1992)

Additional Comments

Genetic Interactions

Statement

Reference

In a sev⁶/Y, Sos^JC2/+ background, the percentage of ommatidia with R7 cells drops in heterozygotes from the 16% of sev⁶/Y, Sos^JC2/+ to 0%.

(Kolodkin et al., 1994)

Flies doubly homozygous for sev⁶ and boss¹ and also heterozygous for Sos^JC2 have no R7 photoreceptor cells.

(Bonfini et al., 1992)

Ommatidia of double mutants with Gap1^B2 show multiple R7 cells. Homozygous mutant phenotype can be suppressed by Gap1^B1, Gap1^B2, Gap1^B3, Gap1^B4 or Gap1^B5.

(Gaul et al., 1992)

Capable of dominantly reverting the Sos^JC2 suppression of the sev⁶ mutant phenotype.

(Rogge et al., 1991)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

boss¹ is rescued by boss^HRP

(Kohyama-Koganeya et al., 2008)

boss¹ is rescued by boss^HRP

(Sunio et al., 1999)

boss¹ is rescued by boss^+t7.5

(Hart et al., 1993)

Not rescued by

boss¹ is not rescued by boss^Exboss-Lac

(Sevrioukov et al., 1998)

Comments

The mutant phenotype of this allele can be completely rescued by boss^+t7.5.

(Hart et al., 1993)

The presence of the P{sevRas1.V12} construct rescues the R7 precursor from transformation into a cone cell and induces the formation of supernumerary R7 cells.

(Fortini et al., 1992)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

boss¹

(Chen and Montell, 2020, Duan et al., 2018, Kohyama-Koganeya et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (20)

Report Sections

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