FlyBase Allele Report: Dmel\Cdk1[hs.PS]

General Information

Symbol

Dmel\Cdk1^hs.PS

Species

D. melanogaster

Name

heat shock construct of Stern

FlyBase ID

FBal0030733

Feature type

allele

Associated gene

Dmel\Cdk1

Associated Insertion(s)

Carried in Construct

P{hs-Cdk1.S}

Also Known As

Hs-Cdk1

Key Links

Transgenic product class

cDNA

(Stern et al., 1993)

wild_type

(Stern et al., 1993)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Stern et al., 1993)

wild_type

(Stern et al., 1993)

Mutagen

in vitro construct

(Sprenger et al., 1997, Stern et al., 1993)

Progenitor genotype

Carried in construct

P{hs-Cdk1.S}

Cytology

Description

An Hsp70 promoter drives expression of a Cdk1 cDNA.

(Stern et al., 1993)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

Cdk1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

wild-type

(Stern et al., 1993)

Phenotype Manifest In

Detailed Description

Statement

Reference

Heatshock induced expression of cdc2^hs.PS has no cell cycle phenotype.

(Parry and O'Farrell, 2001)

Cells of heatshocked embryos carrying cdc2^hs.PS and CycB^S.hs delay mitosis after irradiation as normal.

(Su et al., 2000)

Has no effect on S phase induction in embryos when expressed using heat shock.

(Sprenger et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Cdk1^hs.PS is a suppressor of visible | recessive phenotype of Myb²

(Katzen et al., 1998)

NOT Suppressor of

Statement

Reference

CycB^Δ90, CycB3^Δ, Cdk1^hs.PS is a non-suppressor of abnormal mitotic cell cycle | embryonic stage phenotype of CycA^C8LR1

(Reber et al., 2006)

Phenotype Manifest In

Enhancer of

Statement

Reference

Cdk1^hs.PS is an enhancer of cell cycle phenotype of CycA^S.hs

(Parry and O'Farrell, 2001)

Cdk1^hs.PS is an enhancer of cell cycle phenotype of CycB^S.hs

(Parry and O'Farrell, 2001)

Suppressor of

Statement

Reference

Cdk1^hs.PS is a suppressor of wing hair phenotype of Myb²

(Katzen et al., 1998)

Cdk1^hs.PS is a suppressor of eye phenotype of Zzzz\aptamer^hs.Cdk1.pep8

(Kolonin and Finley, 1998)

NOT Suppressor of

Statement

Reference

CycB^Δ90, CycB3^Δ, Cdk1^hs.PS is a non-suppressor of embryonic epidermis phenotype of CycA^C8LR1

(Reber et al., 2006)

Cdk1^hs.PS is a non-suppressor of phenotype of Eip63E^E1/Eip63E^GN50

(Stowers et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Coexpression of the transgenes, cdc2^hs.PS, CycB^Δ90, and CycB3^Δ, does not restore the entry of epidermal cells in CycA^C8LR1 embryos into mitosis 16.

(Reber et al., 2006)

Co-induction of cdc2^hs.PS and CycA^S.hs leads to a more metaphase arest than seen in CycA^S.hs embryos alone.

(Parry and O'Farrell, 2001)

Myb² mutant pupae carrying cdc2^hs.PS raised at 25^oC exhibit partial suppression of the wing phenotype, the number of hairs is increased by 30-35% and the orientation of the hairs is corrected.

(Katzen et al., 1998)

Heat induced coexpression of CycA^hs.PK and Cdk1^hs.PS fails to induce proliferation in quiescent pole cells.

(Su et al., 1998)

Xenogenetic Interactions

Statement

Reference

Has virtually no effect on the rough eye phenotype caused by overexpression of Zzzz\aptamer^{hs.cdc2c.pep4.T:Ecol\trxA}. Suppresses the rough eye phenotype caused by overexpression of Zzzz\aptamer^{hs.cdc2.pep8.T:Ecol\trxA}.

(Kolonin and Finley, 1998)

Complementation and Rescue Data

Rescues

Cdk1^hs.PS rescues Cdk1^unspecified

(Stern et al., 1993)

Comments

Rescues lethality of embryos deficient for maternal and zygotic cdc2 if heat shocked at 7 hours for 45 minutes. Persistent cyclin A expression in PNS no longer observed.

(Stern et al., 1993)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

91675

w^*; P{hs-Cdk1.S}II

91676

w^*; P{hs-Cdk1.S}68.1

Notes on Origin

Discoverer